Sucrose Esters from Physalis peruviana Calyces with Anti-Inflammatory Activity

Abstract

Physalis peruviana is a native plant from the South American Andes and is widely used in traditional Colombian medicine of as an anti-inflammatory medicinal plant, specifically the leaves, calyces, and small stems in poultice form. Previous studies performed by our group on P. peruviana calyces showed potent anti-inflammatory activity in an enriched fraction obtained from an ether total extract. The objective of the present study was to obtain and elucidate the active compounds from this fraction and evaluate their anti-inflammatory activity in vivo and in vitro. The enriched fraction of P. peruviana was purified by several chromatographic methods to obtain an inseparable mixture of two new sucrose esters named peruviose A (1) and peruviose B (2). Structures of the new compounds were elucidated using spectroscopic methods and chemical transformations. The anti-inflammatory activity of the peruvioses mixture was evaluated using ?-carrageenan-induced paw edema in rats and lipopolysaccharide-activated peritoneal macrophages. Results showed that the peruvioses did not produce side effects on the liver and kidneys and significantly attenuated the inflammation induced by ?-carrageenan in a dosage-dependent manner, probably due to an inhibition of nitric oxide and prostaglandin E2, which was demonstrated in vitro. To our knowledge, this is the first report of the presence of sucrose esters in P. peruviana that showed a potent anti-inflammatory effect. These results suggest the potential of sucrose esters from the Physalis genus as a novel natural alternative to treat inflammatory diseases.

Antibacterial activity of Cordia dentata Poir, Heliotropium indicum Linn and Momordica charantia Linn from the Northern Colombian Coast

“…The MIC and MBC of extracts and fractions were evaluated by the microdilution method with slight modifications [28][29][30], and the Clinical and Laboratory Standards Institute [31]. In brief, a series of ethanol extract and fraction concentrations were prepared with the broth and 100 L of these solutions were added to sterile 96-well plates.…”

Dry Powder Formulation From Physalis Peruviana L. Fruits Extract With Antidiabetic Activity Formulated via Co-Spray Drying

“…The hygroscopicity showed values between 8.84 % and 15.61 %. Experiments 3,4,7,8,11,12,15 and 16 were the particles with the higher proportion of colloidal silicon dioxide that showed the lowest hygroscopicity and this was the factor with the main influence in this variable (Factor F) (table 3). For high concentrations of colloidal silicon dioxide, the extract could be trapped within these solid particles and then it could be almost unavailable for water adsorption [44].…”

“…It is well known that daily consumption of raw fruits of P. peruviana helps to maintain stable glucose level in blood [3][4][5][6]. Several other uses, some of them supported by pharmacological studies, have been attributed to calyxes [7,8], leaves [3], roots [9], and even for the whole plant [2,[10][11][12][13]. Recently, we described peruvioses A-F as the main compounds present in the sticky exudate of the fruits, and the responsible for the observed in vitro α-amylase inhibition activity [14].…”

Development and optimization of microparticles containing a hypoglycemic fraction of calyces from Physalis peruviana

“…peruviana calyces are the main by-product of the harvest and commercialization of the fruit and the study of its pharmacological properties and chemical composition is a great opportunity to give added value to this important crop. The previous investigation has been reported the presence of sucrose esters (Franco et al, 2014) and hydroxycinnamic acids (Vilaplana et al, 2014) as well as a large content of rutin (Quercetin 3-O-rutinoside) as the main flavonoid .…”

“…For its fruits, also has been antidiabetic activity (Mora et al, 2010) as well as inhibition of intestinal alfa amylase enzyme (Rey et al, 2015), immunomodulatory and cytotoxic activity in HeLa and fibroblast cells (MierGiraldo et al, 2017) and anti-pterygium effect by inhibiting fibroblast growth (Pardo et al, 2008). The calyces have been shown hepatoprotective effect (Toro et al, 2013) and antiinflammatory activity related to inhibition of the release of macrophage's proinflammatory cytokines (Martínez et al, 2010) and the inhibition of nitric oxide and prostaglandin E2 (Franco et al, 2014).…”