Sucrose Enhances Anthocyanin Accumulation in Torenia by Promoting Expression of Anthocyanin Biosynthesis Genes

Naing, Aung Htay; Xu, Junping; Park, Kyeung Il; Chung, Mi Young; Kim, Chang Kil

doi:10.3390/horticulturae7080219

Cited by 8 publications

(3 citation statements)

References 29 publications

(69 reference statements)

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“… Hase et al (2010) verified that petunia seedlings treated with 3% sucrose could accumulate significant amount of anthocyanin. A similar phenomenon has been reported in torenia ( Torenia fournieri ) leaves ( Naing et al, 2021 ) and postharvest peaches ( Prunus persica ) peel ( Tian et al, 2022 ). Compared to glucose and fructose, sucrose is the most effective and specific activator of anthocyanin biosynthesis in Arabidopsis seedlings ( Solfanelli et al, 2006 ).…”

Section: Discussionsupporting

confidence: 77%

Sucrose-delaying flower color fading associated with delaying anthocyanin accumulation decrease in cut chrysanthemum

Liu,

Teng,

Xiang

et al. 2023

PeerJ

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As fresh ornamental crops, vase life and post-harvested quality of cut flowers have attracted much attention. Flower color fading is the prominent defect in red and purple cut flowers, especially in cut chrysanthemum which have a relative long vase life. Here, the effect of sucrose on change in anthocyanin contents during the vase life of ‘Dante Purple’ cut chrysanthemum was studied. Results showed that 500 mM sucrose as holding solution could significantly delay the decrease in anthocyanin content and maintain the ornamental value for as long as 38 vase days. Moreover, the sucrose also increased the flower diameter, soluble sugar contents and total antioxidant capacity, while decreasing the malondialdehyde contents. Further studies suggested that the transcript levels of anthocyanin biosynthetic genes and transcription factors, CmMYB6 and CmMYB#7, had continuously decreased during the vase life. The changes in these genes expression patterns was retarded by the sucrose treatment, except for CmMYB#7 which is a repressor of anthocyanin biosynthesis gene expression. The decline in relative expression of CmMYB#7 was accelerated by sucrose. These results have supplied clues to study the mechanism whereby sucrose serves as a signal molecule to regulate anthocyanin biosynthesis.

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Section: Discussionsupporting

confidence: 77%

Sucrose-delaying flower color fading associated with delaying anthocyanin accumulation decrease in cut chrysanthemum

Liu,

Teng,

Xiang

et al. 2023

PeerJ

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“…The synthesis of hypericin and hyperforin compounds in Hypericum perforatum plants is greatly affected by water imbalance and osmotic pressure (David et al, 2016 ; Naik et al, 2016 ; Kladar et al, 2017 ). Moreover, increased levels of sucrose in growth media have been found to induce anthocyanin biosynthesis in callus cultures of Malus domestica (Liu et al, 2017 ) and Torenia fournieri (Naing et al, 2021 ). In Brassica oleracea var.…”

Section: Introductionmentioning

confidence: 99%

Effect of sucrose as an elicitor in increasing quercetin-3-O-rhamnoside (quercitrin) content of chrysanthemum (Chrysanthemum morifolium Ramat) callus culture based on harvest time differences

Setiawati,

Arofah,

Nurzaman

et al. 2023

bta

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Chrysanthemum ( Chrysanthemum morifolium ) contains secondary metabolites, such as flavonoid compounds, especially luteolin-7-glucoside and quercetin-3-O-rhamnoside (quercitrin), in its tissues. Utilizing sucrose as an elicitor through callus culture presents an alternative method to enhance the production of secondary metabolites. This research aimed to determine the best sucrose concentration and harvest time for maximizing quercitrin content in chrysanthemum callus culture. The research employed a completely randomized design with four treatment groups: 0, 30, 45, and 60 g/l of sucrose added to MS medium containing 4 ppm 2,4-dichlorophenoxyacetic acid (2,4-D). Callus samples were harvested on the 15th and 30th days of culture. The observed parameters included callus morphology (color and texture), fresh weight, dry weight, the diameter of the callus, and quercitrin content analyzed using high-performance liquid chromatography. The results showed that all callus cultures exhibited intermediate textures and varied colors, predominantly shades of brown. The treatment involving 45 g/l of sucrose with a 30th-day harvest yielded the highest fresh weight, dry weight, and quercitrin content, namely 2.108 g, 0.051 g, and 0.437 mg/g DW, respectively. Notably, the quercitrin content exhibited a 63.67% increase compared to the control.

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“…At present, the detailed information of sugars serving as signal molecules to regulate anthocyanin biosynthesis largely has not been revealed. A few studies suggested that this process was related to the regulation of gene expression levels involved in anthocyanin biosynthesis ( Weiss, 2000 ; Rolland et al, 2002 ; Rook and Bevan, 2003 ; Mol et al, 2008 ; Naing et al, 2021 ). For example, in radish hypocotyl, the anthocyanin accumulation caused by sucrose treatment was related to higher transcripts of CHS and ANS ( Hara et al, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

Glucose Supply Induces PsMYB2-Mediated Anthocyanin Accumulation in Paeonia suffruticosa ‘Tai Yang’ Cut Flower

Zhang

Li²,

Zhang³

et al. 2022

Front. Plant Sci.

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Tree peony (Paeonia suffruticosa) is a well-known Chinese ornamental plant with showy flower color. However, the color fading problem during vase time seriously blocks its development in the cut flower market. In this study, we found that exogenous glucose supply improved the color quality of P. suffruticosa ‘Tai Yang’ cut flowers with increased total soluble sugar and anthocyanin contents of petals. Besides, the promotion effect of glucose was better than the osmotic control of 3-O-methylglucose (3OMG) treatment and the glucose analog mannose treatment. The structural genes, including PsF3H, PsF3′H, PsDFR, PsAOMT, and PsUF5GT, were remarkably upregulated under glucose treatment. Meanwhile, the regulatory genes, including PsbHLH1, PsbHLH3, PsMYB2, PsWD40-1, and PsWD40-2, also showed a strong response to glucose treatment. Among these five regulatory genes, PsMYB2 showed less response to 3OMG treatment but was highly expressed under glucose and mannose treatments, indicating that PsMYB2 may have an important role in the glucose signal pathway. Ectopic overexpression of PsMYB2 in Nicotiana tabacum resulted in a strong pigmentation in petals and stamens of tobacco flowers accompanied with multiple anthocyanin biosynthetic genes upregulated. More importantly, the overexpression of PsMYB2 enhanced the ability of glucose-induced anthocyanin accumulation in Arabidopsis thaliana seedlings since PsMYB2-overexpressing Arabidopsis showed higher expression levels of AtPAL1, AtCHS, AtF3H, AtF3′H, AtDFR, and AtLDOX than those of wild type under glucose treatment. In summary, we suggested that glucose supply promoted petal coloration of P. suffruticosa ‘Tai Yang’ cut flower through the signal pathway, and PsMYB2 was a key component in this process. Our research made a further understanding of the mechanism that glucose-induced anthocyanin biosynthesis of P. suffruticosa cut flowers during postharvest development, laying a foundation for color retention technology development of cut flowers.

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Sucrose Enhances Anthocyanin Accumulation in Torenia by Promoting Expression of Anthocyanin Biosynthesis Genes

Cited by 8 publications

References 29 publications

Sucrose-delaying flower color fading associated with delaying anthocyanin accumulation decrease in cut chrysanthemum

Sucrose-delaying flower color fading associated with delaying anthocyanin accumulation decrease in cut chrysanthemum

Effect of sucrose as an elicitor in increasing quercetin-3-O-rhamnoside (quercitrin) content of chrysanthemum (Chrysanthemum morifolium Ramat) callus culture based on harvest time differences

Glucose Supply Induces PsMYB2-Mediated Anthocyanin Accumulation in Paeonia suffruticosa ‘Tai Yang’ Cut Flower

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