Sucrose-dependent production of biomass and low-caloric steviol glycosides in adventitious root cultures of Stevia rebaudiana (Bert.)

Ahmad, Naveed; Rab, Abdul; Sajid, Muhammad; Ahmad, Nisar; Fazal, Hina; Ali, Mohammad; Egertsdotter, Ulrika

doi:10.1016/j.indcrop.2021.113382

Cited by 13 publications

(20 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The in vitro cultures are trustworthy for the production of biomass and secondary metabolites due to the homogenous growth of cells in contrast to wild plants, which are exposed to multiple and variable environmental conditions that may cause fluctuation in the synthesis of biomass and important metabolites [ 49 ]. Furthermore, the selection of a proper explant also plays a key role in culture development and the synthesis of metabolites because the polyphenolics are not restricted to specific parts in wild species but rather multiple organs of wild plants [ 1 , 2 ]. In previous studies, the exposure of Stevia in vitro cultures to 0, 20, 40, 60, and 80 mM concentrations of salt (NaCl) negatively regulated the growth of Stevia plants [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

“…In 100 mL flasks, the roots were grown, which were obtained from in vitro plantlets. Media were comprised of 40 mL MS-basal media having different concentrations of NAA (0.5–2.0 mg/L) according to the established protocols of Ahmad et al [ 1 , 53 ]. In an orbital shaker, these flasks were kept (120 rpm; Gallankamp, London, England) at 25 °C for 7 weeks until the formation of adventitious roots cultures.…”

Section: Methodsmentioning

confidence: 99%

“…It belongs to the sunflower family ( Asteraceae ). It is commonly known as sweet leaf or sugar leaf due to its sweet taste [ 1 ]. The Stevia rebaudiana is famous for its sweet taste due to the chemical compounds present in the leaves of Stevia such as Steviol glycosides, which commonly includes steviosides and rebaudioside-A contents [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…The steviol glycoside (stevioside) is 300 times sweeter than commercial sugar. It cannot enter into the bloodstream as it has no receptors and is therefore used as a sugar substitute for diabetic patients [ 1 ]. Steviol glycosides have no reported side effects such as genotoxicity and mutagenicity as compared to marketable sugar.…”

Section: Introductionmentioning

confidence: 99%

“…Stevia rebaudiana is a good source of bulk stevioside, diterpene glycosides, rebaudioside A, rebaudioside B, rebaudioside C, etc. One of the qualities of Stevia rebaudiana is that it is a source of food, as it contains carbohydrates, vitamins, nonessential amino acids, polyphenols, and flavonoids, which play an important role in human health [ 1 ]. A number of sweet herbs/shrubs are present among the genus Stevia , but the sweetest member of this family is Stevia rebaudiana ; apart from stevioside, a number of other sweetness link phytochemicals that have been isolated from their leaves are rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside E, dulcoside A, and steviobioside [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Elicitation of Submerged Adventitious Root Cultures of Stevia rebaudiana with Cuscuta reflexa for Production of Biomass and Secondary Metabolites

Ahmad

Khan

et al. 2021

Molecules

Self Cite

View full text Add to dashboard Cite

Stevia rebaudiana is an important medicinal plant that belongs to the Asteraceae family. The leaves of Stevia rebaudiana are a rich source of many health-promoting agents such as polyphenols, flavonoids, and steviol glycoside, which play a key role in controlling obesity and diabetes. New strategies such as the elicitation of culture media are needed to enhance the productivity of active components. Herein, the Cuscuta reflexa extracts were exploited as elicitors to enhance the productivity of active components. Cuscuta reflexa is one of the parasitic plants that has the ability to elongate very fast and cover the host plant. Consequently, it may be possible that the addition of Cuscuta reflexa extracts to adventitious root cultures (ADR) of Stevia rebaudiana may elongate the root more than control cultures to produce higher quantities of the desired secondary metabolites. Therefore, the main objective of the current study was to investigate the effect of Cuscuta reflexa extract as a biotic elicitor on the biomass accumulation and production of antioxidant secondary metabolite in submerged adventitious root cultures of Stevia rebaudiana. Ten different concentrations of Cuscuta reflexa were added to liquid media containing 0.5 mg/L naphthalene acetic acid (NAA). The growth kinetics of adventitious roots was investigated for a period of 49 days with an interval of 7 days. The maximum biomass accumulation (7.83 g/3 flasks) was observed on medium containing 10 mg/L extract of Cuscuta reflexa on day 49. As the concentration of extract increases in the culture media, the biomass gradually decreases after 49 days of inoculation. In this study, the higher total phenolics content (0.31 mg GAE/g-DW), total flavonoids content (0.22 mg QE/g-DW), and antioxidant activity (85.54%) were observed in 100 mg/L treated cultures. The higher concentration (100 mg/L) of Cuscuta reflexa extract considerably increased the total phenolics content (TPC), total phenolics production (TPP), total flavonoids content (TFC), total flavonoids production (TFP), total polyphenolics content (TPPC), and total polyphenolics production (TPPP). It was concluded that the extract of Cuscuta reflexa moderately improved biomass accumulation but enhanced the synthesis of phenolics, flavonoids, and antioxidant activities. Here, biomass’s independent production of secondary metabolites was observed with the addition of extract. The present study will be helpful to scale up adventitious roots culture into a bioreactor for the production of secondary metabolites rather than biomass accumulation in medicinally important Stevia rebaudiana.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%