Sucrose density gradient centrifugation for efficient separation of
                        engineered nanoparticles from a model organism, Caenorhabditis
                        elegans

Johnson, Monique E.; Bustos, Antonio R. Montoro; Petersen, Elijah J.; Hanna, Shannon K.; Murphy, Karen E.; Yu, Lee L.; Nelson, Bryant C.

doi:10.6028/nist.sp.1200-24

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…Then, 100.00 µL of chilled bacterial culture was slowly aspirated on top of each solution. The gradient solutions in microcentrifuge tubes were centrifuged at 4 • C and 170.00× g for the first 5 min, and then 1250.00× g for another 5 min as described previously [48]. After centrifugation, 100.00 µL from each layer of solutions was collected and placed in a 96-well plate.…”

Section: Density Gradient Centrifugation Separationmentioning

confidence: 99%

Role of Staphylococcus aureus’s Buoyant Density in the Development of Biofilm Associated Antibiotic Susceptibility

Kispert,

Liguori,

Velikaneye

et al. 2024

Microorganisms

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Biofilms are clusters of microorganisms that form at various interfaces, including those between air and liquid or liquid and solid. Due to their roles in enhancing wastewater treatment processes, and their unfortunate propensity to cause persistent human infections through lowering antibiotic susceptibility, understanding and managing bacterial biofilms is of paramount importance. A pivotal stage in biofilm development is the initial bacterial attachment to these interfaces. However, the determinants of bacterial cell choice in colonizing an interface first and heterogeneity in bacterial adhesion remain elusive. Our research has unveiled variations in the buoyant density of free-swimming Staphylococcus aureus cells, irrespective of their growth phase. Cells with a low cell buoyant density, characterized by fewer cell contents, exhibited lower susceptibility to antibiotic treatments (100 μg/mL vancomycin) and favored biofilm formation at air–liquid interfaces. In contrast, cells with higher cell buoyant density, which have richer cell contents, were more vulnerable to antibiotics and predominantly formed biofilms on liquid–solid interfaces when contained upright. Cells with low cell buoyant density were not able to revert to a more antibiotic sensitive and high cell buoyant density phenotype. In essence, S. aureus cells with higher cell buoyant density may be more inclined to adhere to upright substrates.

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Section: Density Gradient Centrifugation Separationmentioning

confidence: 99%

Role of Staphylococcus aureus’s Buoyant Density in the Development of Biofilm Associated Antibiotic Susceptibility

Kispert,

Liguori,

Velikaneye

et al. 2024

Microorganisms

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show abstract

“…In this direction, there is a field to be exploited because there are no standardized procedures to separate or extract CNMs from the biological matrices, especially from the nematode cuticle. However, one protocol was developed to remove non-ingested gold nanoparticles from the C. elegans cuticle and medium surrounding before bioaccumulation studies (Johnson et al, 2017b), this approach could be the basis for this additional research.…”

Section: Caenorhabditis Elegansmentioning

confidence: 99%

Toxicology of carbon nanomaterials in the Caenorhabditis elegans model: current status, characterization, and perspectives for testing harmonization

Côa,

Bortolozzo,

Ávila

et al. 2023

Front. Carbon

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Carbon nanomaterials are promising advanced materials for novel technologies. Therefore, biosafety studies are mandatory to support their safe development, uses, and disposal in sustainable innovation. Traditional toxicological assays are typically expensive, time-consuming, and have low throughput; they have been replaced by new approach methodologies (NAMs) focused on in vitro, in chemico and in silico approaches, along with alternative models. Caenorhabditis elegans has emerged as a complete model organism for predictive toxicology due to its transparent body, short reproductive and life cycles, and fully sequenced genome with high homology with the human genome. In this review, we discuss the current status, state-of-the-art characterization techniques, and scientific gaps in nanotoxicity studies involving the carbon nanomaterials and the C. elegans model considering the last two decades of research. Moreover, we show the existing supportive tools to evaluate the internalization and biodistribution of carbon nanomaterials in C. elegans and discuss their advantages and limitations. Methodological and experimental gaps must still be discussed with the scientific community; hence, we bring this discussion to light and point out future orientations and perspectives. This review will contribute for guiding the research with C. elegans and harmonization of assays/protocols linked to computational tools and nanoinformatics approaches during the development of carbon nanomaterials.

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Combining secondary ion mass spectrometry image depth profiling and single particle inductively coupled plasma mass spectrometry to investigate the uptake and biodistribution of gold nanoparticles in Caenorhabditis elegans

Johnson

Bennett

Bustos

et al. 2021

Analytica Chimica Acta

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Sucrose density gradient centrifugation for efficient separation of engineered nanoparticles from a model organism, Caenorhabditis elegans

Cited by 3 publications

References 22 publications

Role of Staphylococcus aureus’s Buoyant Density in the Development of Biofilm Associated Antibiotic Susceptibility

Role of Staphylococcus aureus’s Buoyant Density in the Development of Biofilm Associated Antibiotic Susceptibility

Toxicology of carbon nanomaterials in the Caenorhabditis elegans model: current status, characterization, and perspectives for testing harmonization

Combining secondary ion mass spectrometry image depth profiling and single particle inductively coupled plasma mass spectrometry to investigate the uptake and biodistribution of gold nanoparticles in Caenorhabditis elegans

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