Sucrose deficiency delays lycopene accumulation in tomato fruit pericarp discs

Télef, Nadège; Stammitti-Bert, Linda; Mortain‐Bertrand, Anne; Maucourt, Mickaël; Carde, Jean‐Pierre; Rolin, Dominique; Gallusci, Philippe

doi:10.1007/s11103-006-9033-y

Cited by 73 publications

(75 citation statements)

References 63 publications

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“…Sucrose was previously shown to promote a color break of citrus fruit epicarp (Iglesias et al 2001). Telef et al (2006) showed that sucrose availability favored lycopene biosynthesis in tomato fruit pericarp disks, and its effect on carotenoid accumulation was stronger than that of hexoses (glucose and fructose).…”

Section: Discussionmentioning

confidence: 99%

Phytochemical characteristics of grafted watermelon on different bottle gourds (Lagenaria siceraria) collected from Mediterranean region of Turkey

Çandır¹

2013

Turk J Agric For

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IntroductionGrafting is widely used in watermelon production to control soil-borne diseases such as Fusarium wilt (Yetisir and Sari 2003;Davis et al. 2008), to increase yield (Lee 1994;Oda 1995;Lee and Oda 2003), and to promote the mineral nutrition uptake (Pulgar et al. 2000), but the objectives of grafting have increased significantly over the years. For instance, grafting has been used to improve resistance against low (Bulder et al. 1990) and high (Rivero et al. 2003) temperatures, to improve resistance against iron chlorosis in calcareous soils (Romero et al. 1997), to improve the salinity tolerance of plants (Yetisir and Uygur 2010), to enhance nutrient absorption (Ruiz et al. 1997), and to improve water use (Cohen and Naor 2002). Bottle gourds have also been used routinely as a source of rootstock for watermelons and other cucurbit crops in some Asian and European countries (Lee and Oda 2003). Lagenaria siceraria is one of the gourd species used as rootstock for watermelon and it shows a high compatibility rate with watermelon (Lee 1994;Oda 1995;Yetisir and Sari 2003). Turkey has a rich genetic diversity of L. siceraria. In a previous study, 210 L. siceraria genotypes from ecologically different regions of Turkey were collected and evaluated for rootstock potential, and it was found that Turkish L. siceraria germplasm has powerful rootstock potential for watermelon against Fusarium wilt and is a good resource for rootstock breeding programs (Yetişir et al. 2007). We previously demonstrated that grafted watermelon plants on bottle gourd rootstocks had an increase in total yield and plant growth parameters as compared to the ungrafted control plants (Karaca et al. 2012). These rootstocks with vigorous root systems also showed superior tolerance to serious soil-borne diseases such as those caused by Fusarium (Yetışır et al. 2003). The rootstocks tested in this study also produced larger fruit with a thicker rind and darker fruit flesh color than the control plants ( Karaca et al. 2012).Watermelon quality is greatly affected by grafting (Yetisir and Sari 2003; Yetışır et al. 2003;Davis and Perkins-Veazie 2005). There are many conflicting reports on changes in fruit quality due to grafting. The differences in reported results may be due in part to different production environments, type of rootstock, interactions between specific rootstocks and scions, and harvest date (Davis et al. 2008). In some studies, grafted watermelon fruits had lower (

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Section: Discussionmentioning

confidence: 99%

Phytochemical characteristics of grafted watermelon on different bottle gourds (Lagenaria siceraria) collected from Mediterranean region of Turkey

Çandır¹

2013

Turk J Agric For

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“…In our study, considerable changes in the metabolite composition have been observed that reside from perturbed carotenoid formation. Other studies have also reported in situ manipulation of sucrose levels altering carotenoid formation (Té lef et al, 2006). Presumably this illustrates the difference between systems that have been intentionally perturbed and unmodified physiological conditions.…”

Section: Online)mentioning

confidence: 97%

Manipulation of Phytoene Levels in Tomato Fruit: Effects on Isoprenoids, Plastids, and Intermediary Metabolism

et al. 2007

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In tomato (Solanum lycopersicum), phytoene synthase-1 (PSY-1) is the key biosynthetic enzyme responsible for the synthesis of fruit carotenoids. To further our understanding of carotenoid formation in tomato fruit, we characterized the effect of constitutive expression of an additional tomato Psy-1 gene product. A quantitative data set defining levels of carotenoid/isoprenoid gene expression, enzyme activities, and metabolites was generated from fruit that showed the greatest perturbation in carotenoid content. Transcriptional upregulation, resulting in increased enzyme activities and metabolites, occurred only in the case of Psy-1, Psy-2, and lycopene cyclase B. For reactions involving 1-deoxy-D-xylulose5-phosphate synthase, geranylgeranyl diphosphate synthase, phytoene desaturase, z-carotene desaturase, carotene isomerase, and lycopene b-cyclase, there were no correlations between gene expression, enzyme activities, and metabolites. Perturbations in carotenoid composition were associated with changes in plastid type and with chromoplast-like structures arising prematurely during fruit development. The levels of >120 known metabolites were determined. Comparison with the wild type illustrated that key metabolites (sucrose, glucose/fructose, and Glu) and sectors of intermediary metabolism (e.g., trichloroacetic acid cycle intermediates and fatty acids) in the Psy-1 transgenic mature green fruit resembled changes in metabolism associated with fruit ripening. General fruit developmental and ripening properties, such as ethylene production and fruit firmness, were unaffected. Therefore, it appears that the changes to pigmentation, plastid type, and metabolism associated with Psy-1 overexpression are not connected with the ripening process.

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“…Isoprenoids were extracted and analyzed as described (Fraser et al, 2000) with slight modification (Te´lef et al, 2006). Briefly, 10 mg of lyophilized sample were mixed using a hand-held homogenizer with 1 mL of methanol and then 1 mL of Tris-HCl buffer (0.05 M, pH 7.5).…”

Section: Extraction and Lc Analysis Of Isoprenoidsmentioning

confidence: 99%

“…The pooled chloroform extracts were dried under a stream of nitrogen, immediately resuspended in 200 lL of ethyl acetate and injected into the HPLC system. Isoprenoid separation, identification and quantification were as described in Te´lef et al (2006).…”

Section: Extraction and Lc Analysis Of Isoprenoidsmentioning

confidence: 99%

Quantitative metabolic profiles of tomato flesh and seeds during fruit development: complementary analysis with ANN and PCA

et al. 2007

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Tomato, an essential crop in terms of economic importance and nutritional quality, is also used as a model species for all fleshy fruits and for genomics of Solanaceae. Tomato fruit quality at harvest is a direct function of its metabolite content, which in turn is a result of many physiological changes during fruit development. The aim of the work reported here was to develop a global approach to characterize changes in metabolic profiles in two interdependent tissues from the same tomato fruits. Absolute quantification data of compounds in flesh and seeds from 8 days to 45 days post anthesis (DPA) were obtained through untargeted (proton nuclear magnetic resonance, 1 H-NMR) and targeted metabolic profiling (liquid chromatography with diode array detection (LC-DAD) or gas chromatography with flame ionization detection (GC-FID)). These data were analyzed with chemometric approaches. Kohonen self organizing maps (SOM) analysis of these data allowed us to combine multivariate (distribution of samples on Kohonen SOMs) and univariate information (component plane representation of metabolites) in a single analysis. This strategy confirmed published data and brought new insights on tomato flesh and seed composition, thus demonstrating its potential in metabolomics. The compositional changes were related to physiological processes occurring in each tissue. They pointed to (i) some parallel changes at early stages in relation to cell division and transitory storage of carbon, (ii) metabolites participating in the fleshy trait and (iii) metabolites involved in the specific developmental patterns of the seeds.

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Sucrose deficiency delays lycopene accumulation in tomato fruit pericarp discs

Cited by 73 publications

References 63 publications

Phytochemical characteristics of grafted watermelon on different bottle gourds (Lagenaria siceraria) collected from Mediterranean region of Turkey

Phytochemical characteristics of grafted watermelon on different bottle gourds (Lagenaria siceraria) collected from Mediterranean region of Turkey

Manipulation of Phytoene Levels in Tomato Fruit: Effects on Isoprenoids, Plastids, and Intermediary Metabolism

Quantitative metabolic profiles of tomato flesh and seeds during fruit development: complementary analysis with ANN and PCA

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