Sucrose content, lignocellulose accumulation and in vitro digestibility of sugarcane internodes depicted in relation to internode maturation stage and Saccharum genotypes

Collucci, Daniel; Bueno, Raphael C.A.; Milagres, Adriane M. F.; Ferraz, André

doi:10.1016/j.indcrop.2019.111543

Cited by 9 publications

(15 citation statements)

References 27 publications

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“…In contrast with previous studies [20,33], no clear correlation was observed between glucan (cellulose + MLG) content and glucose release. This may be due to the positive correlation between cellulose and lignin content in these samples.…”

Section: Enzymatic Saccharificationcontrasting

confidence: 99%

“…Creating more sugarcane fibre that is easier to break down will be key to producing a viable biomaterials sector in the sugarcane industry [9]. Due to the economic importance of the sugarcane culm, the composition of individual internodes has been well characterised between varying levels of maturity and between genotypes [16,25,[30][31][32][33]. The composition in the other important organs of commercial sugarcane, i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Due to the economic importance of the sugarcane culm, the composition of individual internodes has been well characterised between varying levels of maturity, and between genotypes [ 16 , 25 , 30 – 33 ]. The composition in the other important organs of commercial sugarcane, i.e.…”

Section: Introductionmentioning

confidence: 99%

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Variation in sugarcane biomass composition and enzymatic saccharification of leaves, internodes and roots

Mason

Furtado

Marquardt

et al. 2020

Biotechnol Biofuels

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Background The composition of biomass determines its suitability for different applications within a biorefinery system. The proportion of the major biomass fractions (sugar, cellulose, hemicellulose and lignin) may vary in different sugarcane genotypes and growth environments and different parts of the plant. This study investigated the composition of mature and immature internodes, roots and mature leaves of sugarcane. Results Internodes were found to have a significantly larger alcohol-soluble component than leaves and roots. The primary difference between the immature and mature internodes was the ratio of soluble sugars. In mature tissues, sucrose content was significantly higher, whereas in immature internodal tissues there was lower sucrose and heightened concentrations of reducing sugars. Carbon (C) partitioning in leaf tissues was characterised by low levels of soluble components and high “other” and cell wall fractions. Root tissue had low ratios of soluble fractions relative to their cell wall contents, indicating a lack of storage of soluble carbon. There was no significant difference in the ratio of the major cell wall fractions between the major organ types. Characterisation of individual non-cellulosic monomers indicated leaf and root tissues had significantly higher arabinose and galactose fractions. Significantly larger proportions of syringyl lignin compounds and the hydroxycinnamic compound, p-coumaric acid were observed in mature internodal tissues compared to the other tissue types. Tissue-specific differences in composition were shown to greatly affect the recalcitrance of the cell wall to enzymatic saccharification. Conclusions Overall, this study displayed clear evidence of the differential partitioning of C throughout the sugarcane plant in specific organs. These organ-specific differences have major implications in their utility as a bioproduct feedstock. For example, the inclusion of trash (leaves) with the culms (internodes) may alter processing efficiency.

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Section: Enzymatic Saccharificationcontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Variation in sugarcane biomass composition and enzymatic saccharification of leaves, internodes and roots

Mason

Furtado

Marquardt

et al. 2020

Biotechnol Biofuels

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“…The aim of the study was to display essential metabolites that may be used as biomarkers and give information on the mechanism of carbon fixation associated with sugar release and accumulation as lignocellulosic biomass (Mizrachi et al, 2017;Collucci et al, 2019).…”

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confidence: 99%

Metabolic changes in the developing sugarcane culm associated with high yield and early high sugar content

et al. 2020

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Sugarcane is a major crop grown in the tropical and subtropical regions of the world, producing thelargest biomass of any other crop at over 1.9 billion tons in 2018 (FAOSTAT, 2020). Sugarcane has a significant advantage over most other potential biomass crops because of its long history of industry research and development and the existing infrastructure that is currently used for traditional sugar production (Botha & Moore, 2013). Life cycle analyses indicate that sugarcane would be highly competitive with other crops as a preferred feedstock for a biomass-based industry (Renouf et al., 2008; Tilman et al., 2006). In addition to the extensive growing and processing technologies, the sugarcane production systems are underpinned by extensive breeding, research, and development programs producing new sugarcane varieties improved for yield and to overcome problems associated with existing varieties.

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“…The contents of other non-cellulosic-related monosaccharides showed a consistent and significant decrease through time in both cultures, with little or no differences between the control and the induced cells ( Figures 2D-H). These contrasting accumulation patterns between lignin and non-cellulosic polysaccharides were also observed along stem maturation in four experimental sugarcane hybrids (Collucci et al, 2019). These observations suggest that developmental lignification increases cell wall density with a decreased proportion of polysaccharide to lignin ratio (Collucci et al, 2019).…”

Section: Differentiation Of Tes Is Accompanied By Quantitative and Qumentioning

confidence: 63%

Differentiation of Tracheary Elements in Sugarcane Suspension Cells Involves Changes in Secondary Wall Deposition and Extensive Transcriptional Reprogramming

et al. 2020

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Plant lignocellulosic biomass, mostly composed of polysaccharide-rich secondary cell walls (SCWs), provides fermentable sugars that may be used to produce biofuels and biomaterials. However, the complex chemical composition and physical structure of SCWs hinder efficient processing of plant biomass. Understanding the molecular mechanisms underlying SCW deposition is, thus, essential to optimize bioenergy feedstocks. Here, we establish a xylogenic culture as a model system to study SCW deposition in sugarcane; the first of its kind in a C4 grass species. We used auxin and brassinolide to differentiate sugarcane suspension cells into tracheary elements, which showed metaxylem-like reticulate or pitted SCW patterning. The differentiation led to increased lignin levels, mainly caused by S-lignin units, and a rise in p-coumarate, leading to increased p-coumarate:ferulate ratios. RNAseq analysis revealed massive transcriptional reprogramming during differentiation, with upregulation of genes associated with cell wall biogenesis and phenylpropanoid metabolism and downregulation of genes related to cell division and primary metabolism. To better understand the differentiation process, we constructed regulatory networks of transcription factors and SCW-related genes based on co-expression analyses. Accordingly, we found multiple regulatory modules that may underpin SCW deposition in sugarcane. Our results provide important insights and resources to identify biotechnological strategies for sugarcane biomass optimization.

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Sucrose content, lignocellulose accumulation and in vitro digestibility of sugarcane internodes depicted in relation to internode maturation stage and Saccharum genotypes

Cited by 9 publications

References 27 publications

Variation in sugarcane biomass composition and enzymatic saccharification of leaves, internodes and roots

Variation in sugarcane biomass composition and enzymatic saccharification of leaves, internodes and roots

Metabolic changes in the developing sugarcane culm associated with high yield and early high sugar content

Differentiation of Tracheary Elements in Sugarcane Suspension Cells Involves Changes in Secondary Wall Deposition and Extensive Transcriptional Reprogramming

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