Sucrose concentration and volume of liquid medium on the in vitro growth and development of blackberry cv. Tupy in temporary immersion systems

Abstract

Micropropagation of small fruits such as blackberry has been employed due to the need to obtain plants with high phytosanitary quality. Bioreactor technology has been used to improve efficiency in seedling production. Thus, the objective of this work was to evaluate the best culture medium volume and sucrose concentration for blackberry micropropagation in a temporary immersion bioreactor. In vitro blackberry shoots were segmented containing two buds and an internode (1.0 cm) and placed into MS medium supplemented with inositol (0.1 g L-1), BAP (1 mg L-1) and sucrose (10, 20, 30 or 40 g L-1) at different medium volumes (150, 175 and 200 mL). The total length, number of leaves, number of shoots, and number of hyperhydric shoots were evaluated 56 days after start of the project. For blackberry development and propagation in a bioreactor system, the best results were shown at a medium volume of 175 ml and a sucrose concentration of 20 g L-1.

Sucrose concentration alters the physiological potential of in vitro germinated gabiroba seeds

“…Studies performed by Poothong et al (2020) showed that reducing sucrose in MS medium increased the number and length of raspberry buds in culture medium without or with low sucrose concentration. The excess of sucrose in the culture medium can inhibit chlorophyll synthesis and reduce the photosynthetic capacity, which may result in lower carbon gain and dry weight (Ayub et al 2019), as observed in the present study.…”

A novel temporary immersion bioreactor system for large scale multiplication of banana (Rasthali AAB—Silk)

“…Roels et al 21 and Roels et al 22 stated that 10 ml of medium per explant influenced the shoot multiplication in plantain banana. Ayub et al 32 observed that an optimum volume of culture medium significantly increases shoots per explant in micropropagation of blackberry. Ramos-Castellá et al 33 described that 25 ml of media per explant increases the shoot multiplication in Vanila planifolia.…”

The Effects of Different Sucrose Concentrations on The Regeneration Area of Riccia Fluitans L., A Medicinal Aquatic Plant

“…The least number of shoots were detected in 10 g/L sucrose application (2.2±0.1 shoots/explant). Ayub et al [26] cultured the node and internodes of the blackberry (Rubus spp. L.) in a nutrient environment containing 1 mg/L BAP and 10-40 g/L sucrose and obtained the best regeneration values at a concentration of 20 g/L sucrose.…”