Sucrose concentration and blueberry plant density in temporary immersion systems (TIS)

Abstract

The aim was to determine the ideal concentration of sucrose along with the number of plants per container in the face of micropropagation of blueberry under TIS. In the experiment conducted with sucrose concentration (0, 10, 20, e 30 g L-1), we utilized a WPM medium, taking into account 30 explants per container. For the experiment dealing with blueberry plant density, the same medium with 20 g L-1 of sucrose in conjunction with 30, 40, 50 and 60 explants per container was used. After 90 days from the installation of the trial, the following response variables were assessed herein: total length of the plant, number of shoots, length of the largest shoot, number of hyperhydric shoots, length of hyperhydric shoots, and number of healthy leaves. Under a sucrose concentration corresponding to 20 g L-1 the best possible results were obtained. Survival rates of 22 and 80% under the influence of both 10 and 20 g L-1 treatments were reported, pointing out that plants exposed to 20 g L-1 treatment were more amenable to acclimatization. Thus, for the micropropagation of blueberry under TIS the use of 20 g L-1 sucrose and 60 plants/container comes to being highly recommended to increase yield.

GreenTray®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays

“…The extraction of shoots with forceps from in vitro culture containers, as well as from TIS bioreactors, followed by arranging them on sterile surfaces in order to divide them with the help of surgical scalpels and their subsequent introduction into new culture containers, a process known as subculturing in the conventional in vitro culture process, is time-consuming and represents a significant portion the hand labor involved in most in vitro plant culture processes. The components of the actual GreenTray ® TIS bioreactor described herein, which is a further evolution of the ones presented in the past [31,32], make it significantly improved and versatile compared to other commercial TIS bioreactors, such as RITA ® , Matis ® , Plantform TM , SETIS TM [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17], or the TIS developed by several in vitro plant culture laboratories oriented to solve particular propagation objectives for diverse plant species [18][19][20][21][22][23][24][25][26][27][28].…”

“…The best-known commercial ones are RITA ® , its bigger version Matis ® [1][2][3][4][5][6][7], Plantform TM [8][9][10][11][12][13][14], and SETIS TM [15][16][17]. In addition, there are several other TISs developed for particular purposes by in vitro plant culture laboratories [18][19][20][21][22][23][24][25][26][27][28]. With some differential characteristics among them, there are two common properties which make them similar and help to differentiate the GreenTray ® .…”

GreenTray^®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays

“…Extraction of shoots with forceps form the in vitro culture containers, as well as from TIS bioreactors, arranging them on sterile surfaces in order to divide them with the help of surgical scalpels, followed by their introduction into new culture containers, process known as performing a subculture in the conventional in vitro culture process, is time consuming and represents great part of the hand labor expense dedicated to the perform most of the plant in vitro culture processes. The components of the actual GreenTray ® TIS bioreactor described herein, which is a further evolution of the ones presented in the past [32,33], make it significantly improved and versatile compared to other commercial TIS bioreactors, such as RITA ® , Matis ® , Plantform TM , SETIS TM [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20], or the TIS developed by several plant invitro culture laboratories oriented to solve particular propagation objectives for diverse plant species [21][22][23][24][25][26][27][28][29][30][31].…”

“…The best-known commercial ones are RITA ® , its bigger version Matis ® [4][5][6][7][8][9][10], Plantform TM [11][12][13][14][15][16][17], and SETIS TM [18][19][20]. Besides, there are several other TIS developed for their particular purposes by plant invitro culture laboratories [21][22][23][24][25][26][27][28][29][30][31]. With some differential characteristics among them, there are two common properties, which make them similar, and help to differentiate the GreenTray ® .…”

In Vitro Propagation of the Blueberry ‘Blue Suede™’ (Vaccinium hybrid) in Semi-Solid Medium and Temporary Immersion Bioreactors

“…However, bioreactors based on full immersion in liquid medium can cause hyperhydricity, shear stress, and affect the growth and development of the culture negatively [26,27]. Temporary immersion bioreactors (TIBs) can be used to overcome these challenges by allowing for the immersion of the explants in liquid medium for limited intervals rather than permanent immersion [6,13,[28][29][30]. TIBs have also been successfully tested with the lowbush blueberry (Vaccinium angustifolium) cultivar Fundy, 'NB1 and 'QB1 , showing that the shoot number per explant could be three times higher than that on semi-solid medium [17].…”