Suceinyl trialanine p-nitroanilide hydrolytic activities in plasma and the aorta of rabbits experimentally immunized with soluble elastin

Gmiński, Jan; Drózdz, M

doi:10.1016/s0232-1513(11)80139-8

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…Increase of elastolysis has been reported in an animal model of atherosclerosis induced by EDP. 3,5,6,[21][22][23][24][25][26][27] We observed a significant increase in serum elastolytic activity induced by a cholesterolrich diet, lasting for the entire experiment. Lower doses of captopril and both doses of enalapril and quinapril had no significant effect on this increased elastolytic activity.…”

Section: Discussionmentioning

confidence: 64%

“…Other drugs influencing elastolytic activity in experimental atherosclerosis are as follows: calcium channel blockers (nifedipine) and non-selective calcium antagonists: trifluoperazine, fendiline and calcitonin. [27][28][29] We showed that, in cholesterol-fed rabbits and in groups receiving lower doses of tested ACE-I, the serum and aortic thiobarbituric acid reactive species (TBARS) concentrations after six months of experiment were significantly higher than control. The higher dose of captopril (25 mg/kg/day) prevented the cholesterol-induced rise in TBARS concentration.…”

Section: Discussionmentioning

confidence: 99%

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The influence of angiotensin-converting enzyme inhibitors on the aorta elastin metabolism in diet-induced hypercholesterolaemia in rabbits

Wojakowski¹,

Gmiński²,

Siemianowicz³

et al. 2001

J Renin Angiotensin Aldosterone Syst

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

The influence of angiotensin-converting enzyme inhibitors on the aorta elastin metabolism in diet-induced hypercholesterolaemia in rabbits

Wojakowski¹,

Gmiński²,

Siemianowicz³

et al. 2001

J Renin Angiotensin Aldosterone Syst

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“…This sequence binds to the elastin receptor and exerts numerous biological effects (11). An experimental study undertaken in our Department has revealed that rabbits receiving EDP injections develop atherosclerosis (12). In addition, disturbances in elastin metabolism, leading to increased serum levels of EDPs, are considered as one of the risk factors of atherosclerosis.…”

Section: Introductionmentioning

confidence: 99%

Effect of elastin-derived peptides on the production of tissue inhibitor of metalloproteinase-1, -2, and -3 and the ratios in various endothelial cell lines

Siemianowicz

Likus

Francuz

et al. 2015

Experimental and Therapeutic Medicine

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Tissue inhibitors of metalloproteinases (TIMPs) control the activity of metalloproteinases. Elastin-derived peptides (EDPs) are generated as a result of the degradation of elastin fibers. The EDPs bind to the elastin receptor and exert numerous biological effects. The aim of the present study was to compare the production of TIMP-1, TIMP-2 and TIMP-3 and their ratios in human endothelial cells (ECs) derived from three clinically important vascular localizations (coronary arteries, aorta and iliac artery), and evaluate the influence of a well-known EDP, κ-elastin. The highest concentration of TIMP-1 was identified in the aortic ECs, while the lowest concentration was observed in the ECs derived from the coronary artery. The opposite pattern was observed for TIMP-2 production. When the TIMP-3 concentration was analyzed in the three EC lines, no statistically significant differences were observed. Application of κ-elastin was found to decrease the TIMP-1 concentration in the aortic ECs, while an increase in the TIMP-1 concentration was observed in the ECs derived from the iliac artery. The most significant decrease in TIMP-2 concentration following κ-elastin administration was observed in the ECs obtained from the coronary arteries. Furthermore, the highest concentration of κ-elastin resulted in an increase in TIMP-3 production in the ECs derived from the coronary arteries. The following ratios of the TIMP concentrations were calculated: TIMP-1/TIMP-2, TIMP-1/TIMP-3 and TIMP-2/TIMP-3. Each ratio presented different values for the ECs obtained from the various localizations. In the majority of cases, the addition of κ-elastin did not significantly change these proportions. Therefore, these indicators may be characteristic features that can be used to describe ECs in various clinically important vascular localizations.

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“…Edps stimulate the synthesis and release of metalloproteases. in experimental studies, rabbits receiving injections of EDPs developed atherosclerosis (11). Studies indicate that disturbances of elastin metabolism leading to increased serum levels of Edps are one of the risk factors of atherosclerosis.…”

Section: Introductionmentioning

confidence: 99%

Influence of elastin-derived peptides on metalloprotease production in endothelial cells

Siemianowicz

Gmiński

Goss

et al. 2010

Experimental and Therapeutic Medicine

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Matrix metalloproteases (MMPs) are a family of zinc-dependent endopeptidases that degrade extracellular matrix proteins. MMP-1 and MMP-2 are produced by endothelial cells and are involved in specific vascular pathologies, including atherosclerosis and aortal aneurysm. One of the most important differences between these two metalloproteases is the possibility of hydrolysis of elastin and collagen type IV by MMP-2, but not by MMP-1. Elastin-derived peptides are generated as a result of the degradation of elastin fibers. The aim of our study was to compare the production of MMP-1 and MMP-2 in cultured human arterial endothelial cells derived from vascular pathologies localized at three different sites, the coronary artery, iliac artery and aorta, measured as their concentration in cell culture medium. The second aim was to evaluate the influence of κ-elastin (at concentrations 0.1, 0.4, 1.0, 2.5 or 5.0 μg/ml) on the production of the evaluated metalloproteases in three endothelial cell lines. The production of MMP-1 was statistically significantly greater in endothelial cells derived from the aorta compared to that in the endothelium obtained from the coronary and iliac arteries. There were no statistically significant differences in the production of MMP-2 among the endothelial cell lines tested. The addition of κ-elastin at all evaluated concentrations did not statistically significantly influence the concentration of MMP-1 in the cultured coronary artery endothelium. Furthermore, no statistically significant differences were observed in the cultured iliac artery endothelium. In the cultured endothelium derived from the aorta, κ-elastin at concentrations of 0.1 and 0.4 μg/ml significantly increased the amount of MMP-1.

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Suceinyl trialanine p-nitroanilide hydrolytic activities in plasma and the aorta of rabbits experimentally immunized with soluble elastin

Cited by 5 publications

References 13 publications

The influence of angiotensin-converting enzyme inhibitors on the aorta elastin metabolism in diet-induced hypercholesterolaemia in rabbits

The influence of angiotensin-converting enzyme inhibitors on the aorta elastin metabolism in diet-induced hypercholesterolaemia in rabbits

Effect of elastin-derived peptides on the production of tissue inhibitor of metalloproteinase-1, -2, and -3 and the ratios in various endothelial cell lines

Influence of elastin-derived peptides on metalloprotease production in endothelial cells

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