Succinyl methacrylate polymerized in porous-layered phases for open-tubular capillary electrochromatography: Comparison with silica hydride monolayered phases

Chen, Jian‐Lian; Lin, Yi‐Chen

doi:10.1016/j.chroma.2010.04.040

Cited by 20 publications

(33 citation statements)

References 44 publications

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“…4, which shows EOF at a minimum when ACN content is 40-60%. In fact, the concave shape of the curve could be found in various OT-CEC formats, specifically, in capillaries prepared by direct polymer coating, by stepwise fabrication, or by in situ polymerization [8,28,40,41]. The m eo values determined by using the neutral solute DMSO did not increase with an increasing ratio of ACN in the eluent, acting as if a reversed-phase chromatography solvent was not present at all.…”

Section: The Eof Profiles Upon the Ratio Of Acn Modifiermentioning

confidence: 96%

“…3 illustrate the dependence of electroosmotic mobilities (m eo ) on the pH values of the phosphate buffers for a bare, fused-silica capillary, a g-MAPS silanized capillary, a BMA-CNT capillary, and a BMA-MES capillary. The curve for the BMA-MES capillary, which was prepared using succinate methacrylate (MES) monomer instead of MWNTs, was adapted from a previous study [28]. Among the curves, the g-MAPS and BMA-MES capillaries showed a pattern similar to the bare capillary.…”

Section: Eof Profiles At Buffer Phmentioning

confidence: 99%

“…Our previous results showed the superiority of in situ polymerization over the stepwise fabrication starting with a silica hydride capillary and the same acrylate ligands on an OT-CEC column [28]. The in situ approach forms a porous polymeric layer with more functional ligands loaded on the column than the stepwise approach, which only affords a monolayered phase.…”

Section: Introductionmentioning

confidence: 98%

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Multi‐walled carbon nanotube composites with polyacrylate prepared for open‐tubular capillary electrochromatography

Chen

Lin

2010

Electrophoresis

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A method was developed for the chiral separation of dipeptides with two chiral centers. Although all analyzed peptides contain chromophores and they can be detected by UV-adsorption detection without any derivatization, the chiral separations were not achieved for non-derivatized peptides using the same chiral selectors and experimental conditions. In this paper, these dipeptides were precolumn derivatized by using naphthalene-2, 3-dicarboxyaldehyde as a tagging reagent. In the presence of 2-hydroxypropyl-beta-CD and sodium deoxycholate, naphthalene-2,3-dicarboxyaldehyde-tagged dipeptide enantiomers were well resolved by MEKC. Good separation of all enantiomers was obtained within 14 min. The proposed method was applied to chiral separation of dipeptide enantiomers in spiked human serum samples.

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Section: The Eof Profiles Upon the Ratio Of Acn Modifiermentioning

confidence: 96%

Section: Eof Profiles At Buffer Phmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Multi‐walled carbon nanotube composites with polyacrylate prepared for open‐tubular capillary electrochromatography

Chen

Lin

2010

Electrophoresis

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“…6 As an extension on the standard thermal polymerization approach, Xu and Sun 23 have also reported the fabrication of a tentacle-type polymermodified OT column by glycidyl methacrylate (GMA) grafted polymerization, for further modification with various functional groups. However, for all of the above work, the biggest challenge when using thermal polymerization is the formation of a uniform polymer layer of desired thickness, with initiator concentration and polymerization time considered as critical factors for the optimization and reproducibility of the immobilization reaction.…”

Section: Introductionmentioning

confidence: 99%

Controlled Ultraviolet (UV) Photoinitiated Fabrication of Monolithic Porous Layer Open Tubular (monoPLOT) Capillary Columns for Chromatographic Applications

et al. 2012

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ABSTRACT:An automated column fabrication technique that is based on a ultraviolet (UV) light-emitting diode (LED) array oven, and provides precisely controlled "in-capillary" ultraviolet (UV) initiated polymerization at 365 nm, is presented for the production of open tubular monolithic porous polymer layer capillary (monoPLOT) columns of varying length, inner diameter (ID), and porous layer thickness. The developed approach allows the preparation of columns of varying length, because of an automated capillary delivery approach, with precisely controlled and uniform layer thickness and monolith morphology, from controlled UV power and exposure time. The relationships between direct exposure times, intensity, and layer thickness were determined, as were the effects of capillary delivery rate (indirect exposure rate), and multiple exposures on the layer thickness and axial distribution. Layer thickness measurements were taken by scanning electron microscopy (SEM), with the longitudinal homogeneity of the stationary phase confirmed using scanning capacitively coupled contactless conductivity detection (sC4D). The new automated UV polymerization technique presented in this work allows the fabrication of monoPLOT columns with a very high column-to-column production reproducibility, displaying a longitudinal phase thickness variation within ±0.8% RSD (relative standard deviation).

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“…Porous layer open tubular (PLOT) columns (see Figure 1) are a type of open tubular column used in a variety of chromatography applications, and although they find some use in liquid chromatography (LC) [1][2][3][4][5][6][7][8][9], capillary electrochromatography [10][11][12][13][14], and micro solid phase extraction (µ-SPE) [15,16], their main area of application is in gas chromatography (GC) [17,18]. Typically, such columns are fabricated through the immobilisation of spherical particles onto the inner wall of the capillary.…”

Section: Introductionmentioning

confidence: 99%

In Situ Measurement of Polymer Layer Thickness in Porous Layer Open Tubular (PLOT) Columns Using Optical Absorbance in the Near-IR Range

2016

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Abstract:Highly reproducible fabrication of porous layer open tubular (PLOT) structures in fused silica capillaries is often challenging; thus, methods to measure layer thickness growth in real time represent a powerful tool for the production of such columns. The work presented herein demonstrates the application of optical absorbance in the near-infrared (near IR) range for the in-process measurement of polymer layer growth inside fused silica capillaries during the fabrication of PLOT columns. The proposed technique can be used for both on-and off-line measurements of layer thickness for thermal-and photo-initiated polymerisation methods, performed in either polytetrafluoroethylene (PTFE)-or polyimide-coated capillaries. Measurements of layer thickness were carried out at λ 700 nm, using 100 µm and 8 µm optical fibres, yielding relative standard deviation (%RSD) values of 27% and 22%, respectively.

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Succinyl methacrylate polymerized in porous-layered phases for open-tubular capillary electrochromatography: Comparison with silica hydride monolayered phases

Cited by 20 publications

References 44 publications

Multi‐walled carbon nanotube composites with polyacrylate prepared for open‐tubular capillary electrochromatography

Multi‐walled carbon nanotube composites with polyacrylate prepared for open‐tubular capillary electrochromatography

Controlled Ultraviolet (UV) Photoinitiated Fabrication of Monolithic Porous Layer Open Tubular (monoPLOT) Capillary Columns for Chromatographic Applications

In Situ Measurement of Polymer Layer Thickness in Porous Layer Open Tubular (PLOT) Columns Using Optical Absorbance in the Near-IR Range

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