Succinic acid production from sugarcane bagasse hemicellulose hydrolysate by Actinobacillus succinogenes

Abstract

Succinic acid, a four-carbon diacid, has been the focus of many research projects aimed at developing more economically viable methods of fermenting sugar-containing natural materials. Succinic acid fermentation processes also consume CO(2), thereby potentially contributing to reductions in CO(2) emissions. Succinic acid could also become a commodity used as an intermediate in the chemical synthesis and manufacture of synthetic resins and biodegradable polymers. Much attention has been given recently to the use of microorganisms to produce succinic acid as an alternative to chemical synthesis. We have attempted to maximize succinic acid production by Actinobacillus succinogenes using an experimental design methodology for optimizing the concentrations of the medium components. The first experiment consisted of a 2(4-1) fractional factorial design, and the second entailed a Central Composite Rotational Design so as to achieve optimal conditions. The optimal concentrations of nutrients predicted by the model were: NaHCO(3), 10.0 g l(-1); MgSO(4), 3.0 g l(-1); yeast extract, 2.0 g l(-1); KH(2)PO(4). 5.0 g l(-1); these were experimentally validated. Under the best conversion conditions, as determined by statistical analysis, the production of succinic acid was carried out in an instrumented bioreactor using sugarcane bagasse hemicellulose hydrolysate, yielding a concentration of 22.5 g l(-1).

Engineering furfural tolerance in Escherichia coli improves the fermentation of lignocellulosic sugars into renewable chemicals

“…Succinic acid is currently produced from petroleum derived maleic anhydride and can serve as a starting material for synthesis of many commodity chemicals used in plastics and solvents (32). Genetically engineered strains of E. coli (33) and native succinate producers such as Actinobacillus succinogenes (34)(35)(36) and Anaerobiospirillum succiniciproducens (37) have been tested for lignocellulose conversion to succinate. However, fermentation using these strains required costly additional steps (34), nutrient supplementation (33)(34)(35)(36)(37), and mitigation of toxins in hydrolysates by overliming or treating with activated charcoal (33,36).…”

“…Genetically engineered strains of E. coli (33) and native succinate producers such as Actinobacillus succinogenes (34)(35)(36) and Anaerobiospirillum succiniciproducens (37) have been tested for lignocellulose conversion to succinate. However, fermentation using these strains required costly additional steps (34), nutrient supplementation (33)(34)(35)(36)(37), and mitigation of toxins in hydrolysates by overliming or treating with activated charcoal (33,36). Reengineering derivatives of KJ122 using known combinations of furfural resistance traits resulted in strain XW136 that now ferments hemicellulose hydrolysates in mineral salts medium without costly detoxification steps (32 g/L succinic acid with a yield of 0.9 g/g sugars; Fig.…”

Sugarcane bagasse and leaves: foreseeable biomass of biofuel and bio‐products

“…using different cultivation techniques and with a variety of microorganisms. Borges and Pereira 49 employed SB hemicellulosic hydrolysate for succinic acid production by Actinobacillus succinogenes. Singh et al 48 used SB as an inert support for the growth of fungi Aspergillus niger mycelium for gluconic acid production under SSF and a semi-solid state fermentation (SmSF) system with a stabilized mutant strain ORS-4.410.…”

Metabolic capabilities of Actinobacillus succinogenes for succinic acid production

“…Moreover, many of these sources are waste made by other industries and reusing them will lead to reducing environmental pollution. Different sources has been used as renewable carbon substrate, including corn stalk and cotton stalk (Li et al, 2010), cane molasses (Liu et al, 2008), sugarcane baggase (Borges and Pereira, 2011), straw hydrolysate , corn stover (Zheng et al, 2010), sake lees (Chen et al, 2010), corn fiber (Li et al, 2011) and waste yeast hydrolysate (Chen et al, 2011). Many of these cheap renewable sources are mixtures of hexose and pentose after depolymerisation (Jeffries and Jin, 2004).…”