Succinic acid production from sucrose and molasses by metabolically engineered E. coli using a cell surface display system

Ma, Jiangfeng; Li, Feng; Liu, Rongming; Liang, Liya; Ji, Yaliang; Wei, Ce; Jiang, Min; Jia, Honghua; Ouyang, Pingkai

doi:10.1016/j.bej.2014.08.014

Cited by 25 publications

(11 citation statements)

References 34 publications

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“…5 The biorefinery concept presents the possibility of using a common feedstock for multiple products including biochemicals, bioenergy, biofuels and food products. 5,14 Two biorefinery products with promising market growth that have received research attention with the use of molasses as cheap feedstock are succinic acid (SA) [15][16][17] and fructooligosaccharides. 18,19 Bio-based succinic acid, produced from the microbial fermentation of plant-derived sugars, 20 is a platform chemical widely used for high-value niche applications in the food and beverage industries, as well as for high volume products such as plasticizers, polyurethanes, resins, coatings, and more than 30 commercially important products.…”

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confidence: 99%

Revitalizing the sugarcane industry by adding value to A‐molasses in biorefineries

Dogbe

Mandegari

Görgens

2020

Biofuels Bioprod Bioref

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The downward trend of global sugar market prices, as well as rising energy prices, threaten the survival of the South African sugar industry. Operating a single‐stage sugar crystallization process, which produces less crystalline sugar along with sucrose‐rich A‐molasses as a by‐product, to which value can be added in an integrated biorefinery, could be a viable solution for the industry. It would offer a diversification option as opposed to the current three‐stage crystallization process designed to achieve maximum sugar recovery. This study presents a rigorous Aspen Plus simulation development and economic assessment of different biorefinery scenarios producing succinic acid (SA) and short‐chain fructooligosaccharides (scFOS) from A‐molasses at target market sizes of 440 kt/year and 160 kt/year, respectively, considering the production capacity and market price interplays. In all, seven scenarios of single‐ or multi‐product(s) biorefinery annexed to an existing sugar mill were developed and assessed. Due to the integration benefits, all scenarios studied delivered an internal rate of return (IRR) between 24.1–61.1%, significantly above the required discount rate of 9.7%. In particular, co‐production of SA and scFOS syrup at production rates 40.5 kt/year and 13.5 kt/year from 70% and 30% of the A‐molasses, respectively resulted in the IRR of 56.1% and was considered the most attractive scenario based on the diverse bio‐production, economic performance and easy market entry of the products determined by low minimum selling prices. The proposed integration can lead to the sustainable operation of existing sugar mills by utilizing resources more efficiently, employment of product diversification and job creation. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd

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confidence: 99%

Revitalizing the sugarcane industry by adding value to A‐molasses in biorefineries

Dogbe

Mandegari

Görgens

2020

Biofuels Bioprod Bioref

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“…In our previous study, a succinic acid production of 129.21 g L −1 with a productivity of 2.15 g L −1 h −1 was achieved under a same fermentation condition via the overexpression of mgtA in KMG111 to increase the concentration of intracellular Mg 2+ . Ma et al [15] obtained a production of 79.0 g L −1 succinic acid by using a nonrecombinant E. coli strain (AFP11), but their overall productivity of 1.05 g L −1 h −1 is relatively lower, It was also reported that A. succinogenes could produce 39.0 g L −1 succinic acid with a productivity of 3.61 g L −1 h −1 and a yield of 0.85 g g −1 glucose [34] and C. glutamicum is able to produce 146 g L −1 succinic acid in 46 h with a productivity of 3.17 g L −1 h −1 [22]. But, the high productivity was achieved under the high cost fermentation media.…”

Section: Dual-phase Fermentation In a 3-l Bioreactormentioning

confidence: 97%

Enhanced succinic acid productivity by expression of mgtCB gene in Escherichia coli mutant

Wang

et al. 2016

Journal of Industrial Microbiology and Biotechnology

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In this study, a novel engineering Escherichia coli strain (CBMG111) with the expression of mgtCB gene was constructed for the enhanced fermentative production of succinic acid by utilizing the synergetic effect of mgtC gene to improve the growth of strains at the environment of low Mg(2+) concentration and mgtB to enhance the transport of Mg(2+) into cells. After the effect of the expression of the individual genes (mgtA, mgtB, mgtC) on the growth of E. coli was clarified, the fermentative production of succinic acid by CBMG111 was studied with the low-price mixture of Mg(OH)2 and NH3·H2O as the alkaline neutralizer and the biomass hydrolysates as the carbon sources, which demonstrated that the expression of mgtCB gene can significantly increase the productivity of succinic acid (2.97 g L(-1) h(-1)) compared with that by using the engineering strain with the overexpression of mgtA gene.

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“…The Luria-Bertani (LB) broth was used for preculture. The chemical defined fermentation medium [23] was used for anaerobic fermentation. The bacteria used in the experiments were precultured overnight at 37°C for 12 h at 200 rpm.…”

Section: Medium and Growth Conditionmentioning

confidence: 99%

A staged representation electrochemical stimulated strategy to regulate intracellular reducing power for improving succinate production by Escherichia coli AFP111

Zheng

et al. 2021

Biotechnology Journal

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Background: Escherichia coli AFP111 was previously engineered for succinate production by eliminating byproducts of synthesis pathways. Still, the succinate yield is limited due to the insufficient NADH supplement, when fed with glucose. Microbial electrolysis cell (MEC) allows microorganisms to perform unbalanced fermentation by establishing polarized cathode interaction.Methods and Results: In this study, a cathode electrode was used as an additional electron donor to improve succinate synthesis by E. coli AFP111. In MEC with -0.65 V (vs.Ag/AgCl) poised on cathode electrode, 95.72% electrons were transferred into cells via neutral red (NR), and the ratio of NADH/NAD + increased by 2.5-fold. Meanwhile, compared with the control experiment, the value of oxidation-reduction potential (ORP) changed from −240 to −265 mV in MEC, which was beneficial for NADH generation.During two-stage fermentation (no potential growth stage followed by electric stimulation) in MEC, succinate yield was increased by 29.09% (the final yield was 0.71 g g −1 ), and glucose consumption rate was enhanced by 36.22%. In addition, the carbon flux was pumped to succinate and pyruvate metabolism was enhanced. Conclusion and implications:Staged representation of electrochemical stimulated strategy is effective for succinate producing in engineered E. coli by regulating intracellular reducing power, which provides a new concept for producing reduced metabolite in unbalanced fermentation.

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Succinic acid production from sucrose and molasses by metabolically engineered E. coli using a cell surface display system

Cited by 25 publications

References 34 publications

Revitalizing the sugarcane industry by adding value to A‐molasses in biorefineries

Revitalizing the sugarcane industry by adding value to A‐molasses in biorefineries

Enhanced succinic acid productivity by expression of mgtCB gene in Escherichia coli mutant

A staged representation electrochemical stimulated strategy to regulate intracellular reducing power for improving succinate production by Escherichia coli AFP111

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