Succinate dehydrogenase. XVIII. Regulation of succinate dehydrogenase activity by reduced coenzyme Q10

Gutman, Menachem; Eb, Kearney; Tp, Singer

doi:10.1021/bi00790a011

Cited by 74 publications

(3 citation statements)

References 17 publications

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“…The TCA cycle is the major source of mitochondrial NADH. When NADH is exhausted, as expected here with reduction of oxphos ( Figure 1 D), SDH activity is reduced [ 36 ]. This is consistent with our observed reduction in SDH activity with two assays using intact mitochondrial complexes.…”

Section: Discussionsupporting

confidence: 70%

Heme Oxygenase-1 Supports Mitochondrial Energy Production and Electron Transport Chain Activity in Cultured Lung Epithelial Cells

Carr

Garcia

Scaffa

et al. 2020

IJMS

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Heme oxygenase-1 is induced by many cellular stressors and catalyzes the breakdown of heme to generate carbon monoxide and bilirubin, which confer cytoprotection. The role of HO-1 likely extends beyond the simple production of antioxidants, for example HO-1 activity has also been implicated in metabolism, but this function remains unclear. Here we used an HO-1 knockout lung cell line to further define the contribution of HO-1 to cellular metabolism. We found that knockout cells exhibit reduced growth and mitochondrial respiration, measured by oxygen consumption rate. Specifically, we found that HO-1 contributed to electron transport chain activity and utilization of certain mitochondrial fuels. Loss of HO-1 had no effect on intracellular non-heme iron concentration or on proteins whose levels and activities depend on available iron. We show that HO-1 supports essential functions of mitochondria, which highlights the protective effects of HO-1 in diverse pathologies and tissue types. Our results suggest that regulation of heme may be an equally significant role of HO-1.

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Section: Discussionsupporting

confidence: 70%

Heme Oxygenase-1 Supports Mitochondrial Energy Production and Electron Transport Chain Activity in Cultured Lung Epithelial Cells

Carr

Garcia

Scaffa

et al. 2020

IJMS

View full text Add to dashboard Cite

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“…Consequently, the enzyme is obtained largely in the deactivated form, containing tightly bound oxaloacetate in a 1:1 ratio to the enzyme [74]. Ubiquinol, a reduced form of coenzyme Q, is a physiologically relevant reductant and activator of complex II [75, 76]. The highest succinate dehydrogenase activity is observed in state 4 of respiration, when coenzyme Q is largely in the ubiquinol form and capable of reactivating complex II [58].…”

Section: Mitochondria As H2o2 Sourcementioning

confidence: 99%

H2O2 Signalling Pathway: A Possible Bridge between Insulin Receptor and Mitochondria

Помыткин¹

2012

Current Neuropharmacology

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This review is focused on the mechanistic aspects of the insulin-induced H2O2 signalling pathway in neurons and the molecules affecting it, which act as risk factors for developing central insulin resistance. Insulin-induced H2O2 promotes insulin receptor activation and the mitochondria act as the insulin-sensitive H2O2 source, providing a direct molecular link between mitochondrial dysfunction and irregular insulin receptor activation. In this view, the accumulation of dysfunctional mitochondria during chronological ageing and Alzheimer’s disease (AD) is a risk factor that may contribute to the development of dysfunctional cerebral insulin receptor signalling and insulin resistance. Due to the high significance of insulin-induced H2O2 for insulin receptor activation, oxidative stress-induced upregulation of antioxidant enzymes, e.g., in AD brains, may represent another risk factor contributing to the development of insulin resistance. As insulin-induced H2O2 signalling requires fully functional mitochondria, pharmacological strategies based on activating mitochondria biogenesis in the brain are central to the treatment of diseases associated with dysfunctional insulin receptor signalling in this organ.

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“…Succinate dehydrogenase (SDH) catalyses electron transfer from succinate to quinone during aerobic respiration (Singer et al, 1971). Carboxin is a specific inhibitor of this enzyme from several different organisms, including fungi (Broomfield and Hargreaves, 1992;Keon et al, 1994;Topp et al, 2002;Kilaru et al, 2009;Shima et al, 2009Shima et al, , 2011.…”

Section: Introductionmentioning

confidence: 99%

TrichoGate: An Improved Vector System for a Large Scale of Functional Analysis of Trichoderma Genes

et al. 2019

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Species of the genus Trichoderma are ubiquitous in the environment and are widely used in agriculture, as biopesticides, and in the industry for the production of plant cell wall-degrading enzymes. Trichoderma represents an important genus of endophytes, and several Trichoderma species have become excellent models for the study of fungal biology and plant-microbe interactions; moreover, are exceptional biotechnological factories for the production of bioactive molecules useful in agriculture and medicine. Next-generation sequencing technology coupled with systematic construction of recombinant DNA molecules provides powerful tools that contribute to the functional analysis of Trichoderma genetics, thus allowing for a better understanding of the underlying factors determining its biology. Here, we present the creation of diverse vectors containing (i) promoter-specific vectors for Trichoderma, (ii) gene deletions (using hygromycin phosphotransferase as selection marker), (iii) protein localization (mCherry and eGFP, which were codon-optimized for Trichoderma), (iv) gene complementation (neomycin phosphotransferase) and (v) overexpression of encoding gene proteins fused to fluorescent markers, by using the Golden Gate cloning technology. Furthermore, we present the design and implementation of a binary vector for Agrobacterium-mediated transformation in Trichoderma to increase the homologous recombination rate and the generation of a novel selection marker based on carboxin resistance.

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Succinate dehydrogenase. XVIII. Regulation of succinate dehydrogenase activity by reduced coenzyme Q10

Cited by 74 publications

References 17 publications

Heme Oxygenase-1 Supports Mitochondrial Energy Production and Electron Transport Chain Activity in Cultured Lung Epithelial Cells

Heme Oxygenase-1 Supports Mitochondrial Energy Production and Electron Transport Chain Activity in Cultured Lung Epithelial Cells

H2O2 Signalling Pathway: A Possible Bridge between Insulin Receptor and Mitochondria

TrichoGate: An Improved Vector System for a Large Scale of Functional Analysis of Trichoderma Genes

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