Successively amplified electrochemical immunoassay based on biocatalytic deposition of silver nanoparticles and silver enhancement

Electrochemical Dissolution of Silver Nanoparticles and Its Application in Metalloimmunoassay

“…In this approach the antibody is labeled with an enzyme, which hydrolyzes ascorbic acid 2-phosphate to ascorbic acid and this in turn reduces silver ions into metallic silver nanoparticles. The biocatalyticly deposited silver is then dissolved and quantified by ASV [9] or even further enlarged with a silver enhancement procedure [10].…”

“…10 6 from 40-nm particle) and their subsequent detection at a carbon electrode using ASV. The analytical signal can be even further enhanced by deposition of another metal on the surface of nanoparticle, after the immunoreaction has occurred.…”

A highly sensitive signal-amplified gold nanoparticle-based electrochemical immunosensor for dibutyl phthalate detection

“…Likewise, some metal nanoparticles, which possess electronic and catalytic properties to a certain extent and are able to act as labels, have been previously used for signal amplification (Katz et al 2004). These methods have been enhanced by autocatalytic metal deposition to enlarge the sizes of nanoparticles, resulting in a marked improvement in the detection sensitivity (Chen et al 2007;Wang et al 2002).…”

Silver Nanoparticles as a Glucose Oxidase Immobilization Matrix for Amperometric Glucose Biosensor Construction

“…This has led to the construction of electrochemical biosensors with enhanced analytical performance using silver nanoparticles. 8,9 In this work, a glucose biosensor based on thiol-modified silver nanoparticles (AgNPs) was fabricated by immobilization of glucose oxidase onto AgNPs (GOD-AgNPs). The influence of the microenvironment of the GOD-AgNPs on enzyme activity was studied.…”