Successive Construction of Cellulase Hyperproducers of Trichoderma Using Hyperpolyploids

Toyama, Hideo; Toyama, Nobuo

doi:10.1385/abab:84-86:1-9:419

Cited by 10 publications

(2 citation statements)

References 10 publications

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“…Colchicine has been shown to induce polyploidy in plants by inhibiting mitosis (2,3). Colchicine has also been shown to induce polyploidy in fungi and Basidiomycetes (4,5). When fungal conidia are incubated in a liquid medium containing colchicine, the diameter of nuclei in conidia gradually increase and generate polyploid nuclei.…”

Section: Introductionmentioning

confidence: 99%

Filter paper degrading ability of a Trichoderma strain with multinucleate conidia

Toyama

Yano

Hotta

et al. 2007

Appl Biochem Biotechnol

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The multinucleate conidia were produced from the green mature conidia of Trichoderma reesei Rut C-30 strain by colchicine treatment. The strain with higher Filter paper degrading ability was selected among those conidia using a double layer selection medium. The selected strain, JS-2 was able to collapse the filter paper within 15 min but the original strain took 25 min to collapse it completely. Moreover, the amount of reducing sugar in the L-type glass tube of the strain, JS-2 was greater than that of the original strain. The Avicel, CMC-Na, and Salicin hydrolyzing activity of the strain, JS-2, increased 2.1 times, 1.2 times, and 3.6 times higher than that of the original strain.

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Section: Introductionmentioning

confidence: 99%

Filter paper degrading ability of a Trichoderma strain with multinucleate conidia

Toyama

Yano

Hotta

et al. 2007

Appl Biochem Biotechnol

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“…For this purpose, a system must be developed to rapidly isolate cellulase hyperproducers of this fungus. We earlier formed autopolyploids of this fungus using polyploidizer with swollen conidium [5]. Moreover, we could develop a double-layer selection medium that can rapidly isolate the strains with higher degrading ability of crystalline cellulose [6].…”

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Rapid Isolation of the Trichoderma Strain with Higher Degrading Ability of a Filter Paper and Superior Proliferation Characteristics Using Avicel Plates and the Double-Layer Selection Medium

Toyama

Nakano

Satake

et al. 2007

Appl Biochem Biotechnol

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The cost of cellulase is still a problem for bioethanol production. As the cellulase of Trichoderma reesei is applicable for producing ethanol from cellulosic materials, the cellulase productivity of this fungus should be increased. Therefore, we attempted to develop a system to isolate the strain with higher degrading ability of a filter paper and superior proliferation characteristics among the conidia treated with the mitotic arrester, colchicine. When green mature conidia of T. reesei RUT C-30 were swollen, autopolyploidized, and incubated in the double-layer selection medium containing Avicel, colonies appeared on the surface earlier than the original strain. When such colonies and the original colony were incubated on the Avicel plates, strain B5, one of the colonies derived from the colchicine-treated conidia, showed superior proliferation characteristics. Moreover, when strain B5 and the original strain were compared in the filter paper degrading ability and the cellulose hydrolyzing activity, strain B5 was also superior to the original strain. It was suspected that superior proliferation characteristics of strain B5 reflects higher filter paper degrading ability. Thus, we concluded that the Trichoderma strain with higher degrading ability of a filter paper and superior proliferation characteristics can be isolated using Avicel plates and the double-layer selection medium.

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Active Nuclear Shuffling System Using a Swollen Conidium of Trichoderma reesei

Toyama¹,

Yano²,

Gisushi³

et al. 2003

Biotechnology for Fuels and Chemicals

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Successive Construction of Cellulase Hyperproducers of Trichoderma Using Hyperpolyploids

Cited by 10 publications

References 10 publications

Filter paper degrading ability of a Trichoderma strain with multinucleate conidia

Filter paper degrading ability of a Trichoderma strain with multinucleate conidia

Rapid Isolation of the Trichoderma Strain with Higher Degrading Ability of a Filter Paper and Superior Proliferation Characteristics Using Avicel Plates and the Double-Layer Selection Medium

Active Nuclear Shuffling System Using a Swollen Conidium of Trichoderma reesei

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