Succession in arbuscular mycorrhizal fungi can be attributed to a chronosequence of Cunninghamia lanceolata

Abstract

Arbuscular mycorrhizal (AM) fungi play an important role in plant-fungi communities. It remains a central question of how the AM fungal community changes as plants grow. To establish an understanding of AM fungal community dynamics associated with Chinese fir, Chinese fir with five different growth stages were studied and 60 root samples were collected at the Jiangle National Forestry Farm, Fujian Province. A total of 76 AM fungal operational taxonomic units (OTUs) were identified by high-throughput sequencing on an Illumina Miseq platform. The genera covered by OTUs were Glomus, Archaeospora, Acaulospora, Gigaspora and Diversispora. Glomus dominated the community in the whole stage. The number and composition of OTUs varied along with the host plant growth. The number of OTUs showed an inverted V-shaped change with the host plant age, and the maximum occurred in 23-year. Overall, the basic species diversity and richness in this study were stable. Non-metric multi-dimensional scaling (NMDS) analysis based on bray-curtis distance revealed that there were remarkable differentiations between the 9-year and other stages. Besides, AM fungal community in 32-year had a significant difference with that of 23-year, while no significant difference with that of 45-year, suggesting that 32-year may be a steady stage for AM fungi associated with Chinese fir. The cutting age in 32-year may be the most favorable for microbial community. The pH, total N, total P, total K, available N, available P, available K, organic matter and Mg varied as the Chinese fir grows. According to Mantel test and redundancy analysis, available N, available P, K and Mg could exert significant influence on AM fungal communities, and these variables explained 31% of variance in the composition of AM fungal communities.

Alterations in Arbuscular Mycorrhizal Community Along a Chronosequence of Teak (Tectona grandis) Plantations in Tropical Forests of China

“…Similar results for the dominance of Glomus were observed in teak plantations in Thailand using different methods (Chaiyasen et al, 2014(Chaiyasen et al, , 2017. The Glomus was the most abundant genus reported in forest ecosystems (Chen et al, 2007;Lu et al, 2019;Jing et al, 2020;Ji et al, 2021), reclamation land (Ezeokoli et al, 2020), desert vegetative sites (Vasar et al, 2021), agroecosystems (Wang et al, 2020) and saline ecosystems (Sheng et al, 2019) indicating its wide adaptation to diverse ecosystems. Additionally, studies found that the AMV4.5NF/AMDGR primers favored the amplification of Glomeraceae sequences (Luo et al, 2020), which may also have resulted in the dominance of Glomeraceae and Glomus at the family and genus levels, respectively.…”

Revisión: Distribución y efecto de los hongos micorrízicos en el agroecosistema de café

“…Este trabajo muestra algunos de esos datos condensados. La alta diversidad de HMA en el sistema cafetalero puede depender del hospedero (Camargo-Ricalde, Montaño-Arias, De la Rosa-Mera, & Montaño-Arias, 2012), aunque los factores bióticos y abióticos del sitio podrían determinar la sucesión de las especies fúngicas (Lu et al, 2019).…”

Analysis of endophyte diversity of two Gentiana plants species and the association with secondary metabolite

“…Rarefaction analysis based on Mothur v.1.21.1 was conducted to reveal the diversity indices, including goods_coverage, Chao 1 and Shannon [ 19 ]. Non-Metric Multi-Dimensional Scaling (NMDS) analysis were used to analyze the community differences between different samples based on Bray-Curtis [ 20 ]. Metabolic and ecologically relevant functions were annotated by PICRUSt for the 16S rDNA OTU and FUNGuild v1.0 for the ITS OUT [ 21 ].…”