Successful use of oviduct epithelial cell coculture for in vitro production of viable red deer (Cervus elaphus) embryos

Locatelli, Yann; Cognie, Y.; Vallet, J.C.; Baril, Gérard; Verdier, M.; Poulin, Noëlle; Legendre, X.; Mermillod, Pascal

doi:10.1016/j.theriogenology.2005.04.002

Cited by 28 publications

(21 citation statements)

References 34 publications

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“…In addition, after co-incubation of equine gametes with oviduct cells, the IVF rate was not different between porcine and equine oviduct cells. The beneficial effect of homologous oviduct cells on in vitro production of embryos is also demonstrated in human [5,6], bovine [7-9], porcine [10,11], deer [12,13] and dromedary [14] species. Several studies show a positive effect of heterologous oviduct cells on gametes, fertilization and embryo development.…”

Section: Discussionmentioning

confidence: 99%

“…The equine or porcine oviduct epithelial cells were prepared according to Locatelli et al [13]. The oviducts were dissected free from surrounding tissues and washed.…”

Section: Methodsmentioning

confidence: 99%

“…The secretion of oviduct secretory cells increases during the follicular phase under oestrogen and LH stimulation [3,4]. Some studies show that oviduct epithelial cells (OEC) co-culture promotes in vitro production of embryos in human [5,6], bovine[7-9], porcine [10,11], deer [12,13] and dromedary [14]. Moreover, the oviduct proteins have been shown to interact with gametes and to improve efficiency of in vitro fertilization (IVF) in porcine [10,15], bovine [9] and human [16].…”

Section: Introductionmentioning

confidence: 99%

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The secretions of oviduct epithelial cells increase the equine in vitrofertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved?

Mugnier

Kervella

Douet

et al. 2009

Reprod Biol Endocrinol

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BackgroundOviduct epithelial cells (OEC) co-culture promotes in vitro fertilization (IVF) in human, bovine and porcine species, but no data are available from equine species. Yet, despite numerous attempts, equine IVF rates remain low. Our first aim was to verify a beneficial effect of the OEC on equine IVF. In mammals, oviductal proteins have been shown to interact with gametes and play a role in fertilization. Thus, our second aim was to identify the proteins involved in fertilization in the horse.Methods & resultsIn the first experiment, we co-incubated fresh equine spermatozoa treated with calcium ionophore and in vitro matured equine oocytes with or without porcine OEC. We showed that the presence of OEC increases the IVF rates. In the subsequent experiments, we co-incubated equine gametes with OEC and we showed that the IVF rates were not significantly different between 1) gametes co-incubated with equine vs porcine OEC, 2) intact cumulus-oocyte complexes vs denuded oocytes, 3) OEC previously stimulated with human Chorionic Gonadotropin, Luteinizing Hormone and/or oestradiol vs non stimulated OEC, 4) in vivo vs in vitro matured oocytes.In order to identify the proteins responsible for the positive effect of OEC, we first searched for the presence of the genes encoding oviductin, osteopontin and atrial natriuretic peptide A (ANP A) in the equine genome. We showed that the genes coding for osteopontin and ANP A are present. But the one for oviductin either has become a pseudogene during evolution of horse genome or has been not well annotated in horse genome sequence. We then showed that osteopontin and ANP A proteins are present in the equine oviduct using a surface plasmon resonance biosensor, and we analyzed their expression during oestrus cycle by Western blot. Finally, we co-incubated equine gametes with or without purified osteopontin or synthesized ANP A. No significant effect of osteopontin or ANP A was observed, though osteopontin slightly increased the IVF rates.ConclusionOur study shows a beneficial effect of homologous and heterologous oviduct cells on equine IVF rates, though the rates remain low. Furthers studies are necessary to identify the proteins involved. We showed that the surface plasmon resonance technique is efficient and powerful to analyze molecular interactions during fertilization.

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Section: Discussionmentioning

confidence: 99%

“…The equine or porcine oviduct epithelial cells were prepared according to Locatelli et al [13]. The oviducts were dissected free from surrounding tissues and washed.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The secretions of oviduct epithelial cells increase the equine in vitrofertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved?

Mugnier

Kervella

Douet

et al. 2009

Reprod Biol Endocrinol

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“…Its role is of particular importance during the process of fertilization. Oviduct epithelial cells co-culture promotes in vitro production of embryos in human (Bongso et al 1991, Kervancioglu et al 1997, bovine (Chian & Sirard 1995, Way et al 1997, Martus et al 1998, porcine (Romar et al 2001, McCauley et al 2003, deer (Locatelli et al 2005), and dromedary (Khatir et al 2004) species. Moreover, some oviductal proteins have been shown to interact with gametes and to improve efficiency of IVF in porcine (McCauley et al 2003, bovine (Martus et al 1998), human (O' Day-Bowman et al 1996), and equine (Mugnier et al 2009) species.…”

Section: Introductionmentioning

confidence: 99%

Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species

Ambruosi¹,

Accogli²,

Douet³

et al. 2013

Reproduction

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Oviductal environment affects preparation of gametes for fertilization, fertilization itself, and subsequent embryonic development. The aim of this study was to evaluate the effect of oviductal fluid and the possible involvement of deleted in malignant brain tumor 1 (DMBT1) on IVF in porcine and equine species that represent divergent IVF models. We first performed IVF after pre-incubation of oocytes with or without oviductal fluid supplemented or not with antibodies directed against DMBT1. We showed that oviductal fluid induces an increase in the monospermic fertilization rate and that this effect is canceled by the addition of antibodies, in both porcine and equine species. Moreover, pre-incubation of oocytes with recombinant DMBT1 induces an increase in the monospermic fertilization rate in the pig, confirming an involvement of DMBT1 in the fertilization process. The presence of DMBT1 in the oviduct at different stages of the estrus cycle was shown by western blot and confirmed by immunohistochemical analysis of ampulla and isthmus regions. The presence of DMBT1 in cumulus-oocyte complexes was shown by western blot analysis, and the localization of DMBT1 in the zona pellucida and cytoplasm of equine and porcine oocytes was observed using immunofluorescence analysis and confocal microscopy. Moreover, we showed an interaction between DMBT1 and porcine spermatozoa using surface plasmon resonance studies. Finally, a bioinformatic and phylogenetic analysis allowed us to identify the DMBT1 protein as well as a DMBT1-like protein in several mammals. Our results strongly suggest an important role of DMBT1 in the process of fertilization.

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“…Além disso, enzimas antioxidantes, tais como a glutationa-peroxidase, superóxido-dismutase-Cu-Zn e catalase foram descritas em secreções de ovidutos de vacas 15 .…”

Section: Referencial Teóricounclassified

Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados

et al. 2015

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Cómo citar este artículo: Valleriote P, Paes de Carvalho C, Dubeibe DF, Mogollón-Waltero ÉM, Burla-Dias, ÂJ. Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados. Spei Domus. 2015;11(22) Resumo. Introdução: nos bovinos, o oviduto é fundamental nos processos de maturação, fertilização e aquisição de competências para o adequado desenvolvimento do embrião até blastocisto. Visto que as células do revestimento interno do oviduto proporcionam substâncias que afetam positivamente os espermatozoides, à medida que atravessam o trato genital da fêmea, os componentes da superfície são modificados ou removidos pelas secreções ali presentes; ditas modificações podem incluir depleção do colesterol na superfície espermática, alteração nos glicosaminoglicanos e mudanças nos íons. Metodologia: Objetivou-se avaliar a adição de meio de cultura de células de oviduto bovino sobre parâmetros espermáticos como motilidade, vigor e integridade de membrana citoplasmática. Os espermatozoides foram incubados com 164 μl de Talp-sp-Uso no grupo controle (T Ct ); 161,5 μl de Talp-sp-Uso, acrescido de 2,5 μl de heparina (20µg/ml) no tratamento 1 (T Hep ) e 128 μl de Talp-sp uso, acrescido de 36 μl de meio de cultivo com secreções de células do oviduto bovino no Tratamento 2 (T Ov ). Foram avaliados no tempo zero (para mostrar que os meios dos tratamentos não prejudicaram os espermatozoides) e após três horas de incubação. Resultados: não foram encontradas diferenças significativas na motilidade progressiva, vigor e integridade de membrana celular entre tratamentos. Entretanto, espermatozoides cultivados com secreções de células do oviduto apresentaram maior percentagem de motilidade total às três horas de cultivo, em comparação a espermatozoides cultivados na presença de heparina. Conclusão: as secreções de células do oviduto não afetam a motilidade progressiva nem o vigor ou a integridade da membrana citoplasmática dos espermatozoides, porém prolongam a motilidade dessas células.Palavras-chave: capacitação espermática, células do oviduto, meio condicionado.

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Successful use of oviduct epithelial cell coculture for in vitro production of viable red deer (Cervus elaphus) embryos

Cited by 28 publications

References 34 publications

The secretions of oviduct epithelial cells increase the equine in vitrofertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved?

The secretions of oviduct epithelial cells increase the equine in vitrofertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved?

Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species

Influência das secreções de células do oviduto cultivadas in vitro sobre a viabilidade de espermatozoides bovinos congelados-descongelados

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