Successful ultrarapid cryopreservation of wild Iberian ibex (Capra pyrenaica) spermatozoa

Pradieé, J.; Esteso, M.C.; Toledano-Dı́az, A.; Castaño, C.; Carrizosa, J.; Urrutia, B.; Santiago-Moreno, J.

doi:10.1016/j.theriogenology.2015.07.036

Cited by 38 publications

(28 citation statements)

References 64 publications

(2 reference statements)

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“…One way of avoiding damage caused by crystal formation is semen vitrification [5], which has been studied in different animal species and in humans as an alternative to conventional freezing [1,[5][6][7][8][9][10]. Vitrification has been used widely for embryo storage [3] and has been a scope of research in the last decade for sperm cryopreservation [11].…”

Section: Introductionmentioning

confidence: 99%

Vitrification Using Soy Lecithin and Sucrose: A New Way to Store the Sperm for the Preservation of Canine Reproductive Function

Pipan

Casal

Šterbenc

et al. 2020

Animals

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Simple Summary: Soy lecithin and sucrose were used at different concentrations to develop and compare different vitrification methods for the cryopreservation of canine semen. All of the sperm quality characteristics were effectively preserved after devitrification when vitrification extenders containing soy lecithin at 1% and 0.25 M sucrose were used. The results suggest that vitrification is effective, fast, and simple for cryopreservation of canine semen. Furthermore, the use of soy lecithin in lieu of animal proteins (e.g., egg yolk) facilitates semen shipping to countries with strict import requirements.Abstract: A challenge in freezing semen for short and long-term availability is avoiding damage to intact spermatozoa caused by the freezing process. Vitrification protocols provide better results through less manipulation of semen and shorter freezing time compared to slow freezing techniques. Our research was aimed at improving vitrification methods for canine semen. Semen quality was determined in 20 ejaculates after collection. Each ejaculate was divided into eight aliquots, each with a different extender. The control extender contained TRIS, citric acid, fructose, and antibiotics. Soy lecithin and sucrose were added to the control extender at different concentrations to make up the test extenders and final concentration of 50 × 10 6 spermatozoa/mL. From each group, a 33 µL (1.65 × 10 6 spermatozoa) suspension of spermatozoa was dropped directly into liquid nitrogen and devitrified at least one week later and evaluated as before. Soy lecithin at 1% and 0.25 M sucrose added to the base vitrification media effectively preserved all sperm qualities. Our results demonstrate the effectiveness of our methods. Vitrification media containing sucrose and soy lecithin cause a minimal decline in quality of canine semen after devitrification. Furthermore, extenders used in our research did not contain egg yolk, which was replaced by soy lecithin, thus allowing for ease of shipping to other countries with strict requirements.

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Section: Introductionmentioning

confidence: 99%

Vitrification Using Soy Lecithin and Sucrose: A New Way to Store the Sperm for the Preservation of Canine Reproductive Function

Pipan

Casal

Šterbenc

et al. 2020

Animals

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“…These results are in agreement with previous reports in which protein and sucrose addition to a base extender had a cryoprotective effect on motility after vitrification (Isachenko et al., ). The same combination of non‐permeable cryoprotectants has been successfully used for the sperm vitrification of small volumes (from 1 to 30 μl) in different animal species (Isachenko et al., ; Merino et al., ; Pradiee et al., ; Sánchez et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Sperm vitrification has been recently used as an alternative to conventional freezing of human (Isachenko, Isachenko, Sanchez, Katkov, & Kreienberg, ), dog (Sánchez et al., ), fish (Figueroa et al., ; Merino et al., ) and wild goat (Pradiee et al., ) spermatozoa. This technique consists of plunging small volumes of semen samples directly into liquid nitrogen, thus the sample is cooled at ultra‐fast speed rates in the absence of permeable cryoprotectants (Isachenko, Isachenko, et al., ).…”

Section: Introductionmentioning

confidence: 99%

Effect of different extenders for donkey sperm vitrification in straws

Díaz-Jiménez

Pereira

Ortiz

et al. 2017

Reprod Domestic Animals

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Contents Aseptic vitrification of semen samples packed in straws has been successfully developed in human but not in donkeys. The aim of this study was to compare the effect of two extenders for donkey sperm vitrification using straws. Ejaculates from four Andalusian donkeys were collected, and samples were extended in INRA‐96 (I) or Gent (G) supplemented with sucrose 0.25 M and 1% bovine serum albumin (BSA). Extended samples were cooled for one hour at 5°C. For vitrification, samples were filled in covered 0.25 ml straws and then plunged directly into liquid nitrogen. For warming, straws were immersed in INRA‐96 at 43°C. Results showed no significant differences between I and G treatments for TM (34.2% ± 8.7 vs. 30.7% ± 9.6) and PM (26.8% ± 7.3 vs. 24.6% ± 7.9), respectively. In conclusion, donkey sperm could be vitrified in straws either with INRA‐96 or with Gent in combination with sucrose and BSA.

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“…Other significant advances in sperm technologies include cryopreservation by vitrification (22, 23), refreezing of spermatozoa (24–27), and production of spermatozoa from frozen testicular tissue (28, 29). Development in spermatogonia stem cell (SSC) culture opens a new era in the understanding of testis function and male fertility (2, 30–32).…”

Section: Male Gamete Preservation and Artificial Inseminationmentioning

confidence: 99%

Grand Challenge Animal Reproduction-Theriogenology: From the Bench to Application to Animal Production and Reproductive Medicine

Tibary

2017

Front. Vet. Sci.

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Successful ultrarapid cryopreservation of wild Iberian ibex (Capra pyrenaica) spermatozoa

Cited by 38 publications

References 64 publications

Vitrification Using Soy Lecithin and Sucrose: A New Way to Store the Sperm for the Preservation of Canine Reproductive Function

Vitrification Using Soy Lecithin and Sucrose: A New Way to Store the Sperm for the Preservation of Canine Reproductive Function

Effect of different extenders for donkey sperm vitrification in straws

Grand Challenge Animal Reproduction-Theriogenology: From the Bench to Application to Animal Production and Reproductive Medicine

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