Successful Treatment of South American Rattlesnake (Crotalus durissus terrificus) Envenomation with Crotalidae Polyvalent Immune Fab (CroFab™)

Lynch, Michael J.; Ritter, Seth C.; Cannon, Robert D.

doi:10.1007/s13181-010-0100-z

Cited by 8 publications

(3 citation statements)

References 7 publications

(11 reference statements)

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“…Antigen-specific polyclonal antibodies are generated for a wide array of purposes which range from fundamental laboratory studies and protocols to passive immunotherapy for life threatening conditions including snake envenomation [1] and drug toxicity [2]. Sheep are particularly attractive for the generation of passive polyclonal immunotherapeutics as ovine antibody fragments have demonstrated reduced immunogenicity and more consistent biological function than those derived from other animals [3].…”

Section: Introductionmentioning

confidence: 99%

An Empirical Approach towards the Efficient and Optimal Production of Influenza-Neutralizing Ovine Polyclonal Antibodies Demonstrates That the Novel Adjuvant CoVaccine HT™ Is Functionally Superior to Freund's Adjuvant

et al. 2013

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Passive immunotherapies utilising polyclonal antibodies could have a valuable role in preventing and treating infectious diseases such as influenza, particularly in pandemic situations but also in immunocompromised populations such as the elderly, the chronically immunosuppressed, pregnant women, infants and those with chronic diseases. The aim of this study was to optimise current methods used to generate ovine polyclonal antibodies. Polyclonal antibodies to baculovirus-expressed recombinant influenza haemagglutinin from A/Puerto Rico/8/1934 H1N1 (PR8) were elicited in sheep using various immunisation regimens designed to investigate the priming immunisation route, adjuvant formulation, sheep age, and antigen dose, and to empirically ascertain which combination maximised antibody output. The novel adjuvant CoVaccine HT™ was compared to Freund’s adjuvant which is currently the adjuvant of choice for commercial production of ovine polyclonal Fab therapies. CoVaccine HT™ induced significantly higher titres of functional ovine anti-haemagglutinin IgG than Freund’s adjuvant but with fewer side effects, including reduced site reactions. Polyclonal hyperimmune sheep sera effectively neutralised influenza virus in vitro and, when given before or after influenza virus challenge, prevented the death of infected mice. Neither the age of the sheep nor the route of antigen administration appeared to influence antibody titre. Moreover, reducing the administrated dose of haemagglutinin antigen minimally affected antibody titre. Together, these results suggest a cost effective way of producing high and sustained yields of functional ovine polyclonal antibodies specifically for the prevention and treatment of globally significant diseases.

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Section: Introductionmentioning

confidence: 99%

An Empirical Approach towards the Efficient and Optimal Production of Influenza-Neutralizing Ovine Polyclonal Antibodies Demonstrates That the Novel Adjuvant CoVaccine HT™ Is Functionally Superior to Freund's Adjuvant

et al. 2013

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“…Though ovine-origin products carry a risk of contamination with transmissible prion proteins, sourcing of ovine serum from countries free from known prion disease such as Australia and New Zealand can remove this risk, and thus commercially available ovine pAb products are commonly sourced from regions such as these. Ovine pAb fragments have been used with success in the clinic, and are currently available as critical care therapies for life-threatening digoxin toxicity (DigiFab™, DIGIBIND™) 26 and crotalid snake envenomation (CroFab™) 27 .…”

mentioning

confidence: 99%

Preserved antiviral adaptive immunity following polyclonal antibody immunotherapy for severe murine influenza infection

Stevens

Hatjopolous

Fraser

et al. 2016

Sci Rep

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Passive immunotherapy may have particular benefits for the treatment of severe influenza infection in at-risk populations, however little is known of the impact of passive immunotherapy on the formation of memory responses to the virus. Ideally, passive immunotherapy should attenuate the severity of infection while still allowing the formation of adaptive responses to confer protection from future exposure. In this study, we sought to determine if administration of influenza-specific ovine polyclonal antibodies could inhibit adaptive immune responses in a murine model of lethal influenza infection. Ovine polyclonal antibodies generated against recombinant PR8 (H1N1) hemagglutinin exhibited potent prophylactic capacity and reduced lethality in an established influenza infection, particularly when administered intranasally. Surviving mice were also protected against reinfection and generated normal antibody and cytotoxic T lymphocyte responses to the virus. The longevity of ovine polyclonal antibodies was explored with a half-life of over two weeks following a single antibody administration. These findings support the development of an ovine passive polyclonal antibody therapy for treatment of severe influenza infection which does not affect the formation of subsequent acquired immunity to the virus.

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“…Crotalus durissus is the main rattlesnake specie distributed in South America and is responsible for several snakebite accidents with considerable mortality rate. This is due to an intense systemic action characterized by prominent effects as neurotoxicity, coagulation disturbs and myalgic symptoms, leading to end-organs failure and death [1–3]. Crotalus durissus is also classified into subspecies, with variability in venom compositions that may result in differences of intensity and characteristics related to the clinical manifestations [2, 4].…”

Section: From Toxicology To Medicinal Immunopharmacologymentioning

confidence: 99%

Immunotherapeutic potential of Crotoxin: anti-inflammatory and immunosuppressive properties

Sartim

Menaldo

Sampaio

2018

J Venom Anim Toxins Incl Trop Dis

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For the past 80 years, Crotoxin has become one of the most investigated isolated toxins from snake venoms, partially due to its major role as the main toxic component in the venom of the South American rattlesnake Crotalus durissus terrificus. However, in the past decades, progressive studies have led researchers to shift their focus on Crotoxin, opening novel perspectives and applications as a therapeutic approach. Although this toxin acts on a wide variety of biological events, the modulation of immune responses is considered as one of its most relevant behaviors. Therefore, the present review describes the scientific investigations on the capacity of Crotoxin to modulate anti-inflammatory and immunosuppressive responses, and its application as a medicinal immunopharmacological approach. In addition, this review will also discuss its mechanisms, involving cellular and molecular pathways, capable of improving pathological alterations related to immune-associated disorders.

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Successful Treatment of South American Rattlesnake (Crotalus durissus terrificus) Envenomation with Crotalidae Polyvalent Immune Fab (CroFab™)

Cited by 8 publications

References 7 publications

An Empirical Approach towards the Efficient and Optimal Production of Influenza-Neutralizing Ovine Polyclonal Antibodies Demonstrates That the Novel Adjuvant CoVaccine HT™ Is Functionally Superior to Freund's Adjuvant

An Empirical Approach towards the Efficient and Optimal Production of Influenza-Neutralizing Ovine Polyclonal Antibodies Demonstrates That the Novel Adjuvant CoVaccine HT™ Is Functionally Superior to Freund's Adjuvant

Preserved antiviral adaptive immunity following polyclonal antibody immunotherapy for severe murine influenza infection

Immunotherapeutic potential of Crotoxin: anti-inflammatory and immunosuppressive properties

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