Successful treatment of photo-damaged skin of nano-scale atRA particles using a novel transdermal delivery

Yamaguchi, Yoko; Nagasawa, Teruaki; Nakamura, Natsumi; Takenaga, Mitsuko; Mizoguchi, Masahiro; Kawai, Shinichi; Mizushima, Yutaka; Igarashi, Rie

doi:10.1016/j.jconrel.2004.11.036

Cited by 68 publications

(46 citation statements)

References 16 publications

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“…14,15) The present findings suggest that cationic liposomes also have the potential for efficient delivery of retinoic acid and other lipophilic drugs into the skin, although the depth of delivery of the cationic liposomes in the skin remains to be clarified. Since it has been suggested that the size of the liposomes is also important for the interaction of liposomes with the skin, 16) the effects of the particle size of the liposomes on the delivery should also be considered.…”

Section: Resultsmentioning

confidence: 76%

Enhanced Delivery of Retinoic Acid to Skin by Cationic Liposomes

Kitagawa

Kasamaki

2006

CHEMICAL & PHARMACEUTICAL BULLETIN

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Various kinds of double-chained cationic surfactants have been shown to form bilayer vesicles (cationic liposomes) and have been applied to gene delivery.1,2) Cationic vesicles designed for that purpose are generally intrinsically unstable. 3,4) Although application of cationic liposomes has also been anticipated for the delivery of drugs, peptides, vitamins and lipids and other biologically active compounds to biological cells and tissues, liposomes used for that purpose need to be intrinsically stable in order to retain their contents. 5) In a previous study we revealed that phosphatidylcholines enriched with unsaturated acyl chains stabilized the liposomes of dimethyldialkylammoniums such as dimethyldipalmitylammonium at physiological temperature.6) In contrast, 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) formed stable vesicles at physiological temperature with phosphatidylcholines containing either saturated acyl chains such as dimyristoylphosphatidylcholine (DMPC) or unsaturated acyl chains such as dilinoleoylphosphatidylcholine (DLPC). 7) We also revealed that these cationic liposomes interacted with erythrocytes as cationic vesicles and induced the formation of tightly aggregated structures of several erythrocytes which might also induce fusion of the erythrocytes. 6,7)The lipid lamella of the stratum corneum of the skin contains a high ratio of negatively charged lipids, 8) which are expected to interact with cationic liposomes. Transfer of some of the bilayer components of the liposomes to the skin is then possibly induced. Therefore, in this work we prepared cationic liposomes containing retinoic acid (all-trans-retinoic acid) as a bilayer component, and examined the incorporation of retinoic acid into skin by the cationic liposomes. Although topical retinoic acid has been clinically used with success for the treatment of several cutaneous diseases such as psoriasis or ichthyosis and mild acne, 9) its efficient delivery into skin has not been established yet. We compared the incorporation efficiency by cationic liposomes with those by electrically neutral liposomes and elastic liposomes as well as that by an organic solvent solution. ExperimentalMaterials Chloride salt of DOTAP was purchased from Avanti Polar Lipids (Alabaster, AL, U.S.A.). Bromide salt of dimethyldipalmitylammonium was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Dimyristoryphosphatidylcholine (DMPC, L-a -phosphatidylcholine dimyristoyl) and Dipalmitoylphosphatidylcholine (DPPC, L-a -phosphatidylcholine dipalmitoyl) were from Sigma Chemical Co. (St. Louis, MO, U.S.A.). All-trans-retinoic acid, egg yolk phosphatidylcholine (egg yolk PC), heptaethylene glycol mono-n-dodecyl ether and all other reagents were from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).Preparation of Sonicated Liposomes Consisting of Phosphatidylcholines, Double-Chained Cationic Surfactants and Retinoic Acid Liposomes consisting of phosphatidylcholines, DOTAP (or dimethyldipalmitylammonium) and retinoic acid were prepared as described ...

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Section: Resultsmentioning

confidence: 76%

Enhanced Delivery of Retinoic Acid to Skin by Cationic Liposomes

Kitagawa

Kasamaki

2006

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…O ácido retinóico é biologicamente o mais ativo dos retinóides e desempenha importante papel na regulação da expressão gênica na diferenciação celular e proliferação de células epiteliais (SHAPIRO; SALIOU, 2001). Embora o tratamento com este fármaco apresente vários benefícios, estes são freqüentemente acompanhados de excesso de irritação na pele (OLSEN et al, 1992;YAMAGUCHI et al, 2005). As respostas clínicas predominantemente incluem dermatite severa em vários estágios da aplicação (ao redor de 3 ou 4 dias).…”

Section: Estudo In Vitro Do Perfil De Liberaçãounclassified

“…As respostas clínicas predominantemente incluem dermatite severa em vários estágios da aplicação (ao redor de 3 ou 4 dias). Em geral, esta inflamação pode ocorrer por pelo menos dois mecanismos (YAMAGUCHI et al, 2005;NJAR et al, 2006):…”

Section: Estudo In Vitro Do Perfil De Liberaçãounclassified

Desenvolvimento, caracterização e avaliação de sistemas microestruturados para veiculação de ácido retinóico na pele

Lira¹

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“…This has obvious potential applications in skin care products, and in this study we targeted our research towards achieving such applications. We first sought to determine whether the LCG could be effective at concentrations low enough to be practical using the thickening of mouse epidermis as an assay for accelerated epidermal turnover, as described previously [2,5,6]. Following that, we directly quantified the ability of the LCG to erase age spots from human facial skin by analyzing before and after photographs and quantifiably measuring changes in skin pigmentation.…”

Section: Phase Transitionsmentioning

confidence: 99%

Liquid Crystal Gel Reduces Age Spots by Promoting Skin Turnover

Musashi¹,

Coler‐Reilly

Nagasawa³

et al. 2014

Cosmetics

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Studies have shown that liquid crystals structurally resembling the intercellular lipids in the stratum corneum can beneficially affect the skin when applied topically by stimulating the skin's natural regenerative functions and accelerating epidermal turnover. In the present study, the effects of applying low concentrations of a liquid crystal gel of our own creation were evaluated using epidermal thickening in mouse skin as an assay for effective stimulation of epidermal turnover. A liquid crystal gel was also applied topically to human facial skin, and analysis was conducted using before-and-after photographs of age spots, measurements of L* values that reflect degree of skin pigmentation, single-layer samples of the stratum corneum obtained via tape-stripping, and measurements of trans-epidermal water loss that reflect the status of the skin's barrier function. The results suggested that cost-effective creams containing as low as 5% liquid crystal gel might be effective and safely sold as skin care products targeting age spots and other problems relating to uneven skin pigmentation.

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Successful treatment of photo-damaged skin of nano-scale atRA particles using a novel transdermal delivery

Cited by 68 publications

References 16 publications

Enhanced Delivery of Retinoic Acid to Skin by Cationic Liposomes

Enhanced Delivery of Retinoic Acid to Skin by Cationic Liposomes

Desenvolvimento, caracterização e avaliação de sistemas microestruturados para veiculação de ácido retinóico na pele

Liquid Crystal Gel Reduces Age Spots by Promoting Skin Turnover

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