Successful treatment of disseminated nocardiosis diagnosed by metagenomic next-generation sequencing: A case report and review of literature

Li, Ting; Chen, Yixin; Lin, Jiajia; Lin, Weixian; Zhang, Weizhen; Dong, Hangming; Cai, Shaoxi; Meng, Ying Shirley

doi:10.12998/wjcc.v10.i28.10120

Cited by 5 publications

(3 citation statements)

References 36 publications

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“…Positive results were obtained from organs such as kidneys, lungs, bladder, tonsils, ileocecal valve, brain, and liver. The presence of Nocardia in different organs is consistent with disseminated nocardiosis (Saubolle and Sussland, 2003;Li et al, 2022). Cerebral nocardiosis is a rare entity in humans, but has been reported (Sayer et al, 2022;Sher et al, 2022).…”

Section: Discussionmentioning

confidence: 56%

Molekularni probir Nocardia spp. u divljih sisavaca - presječna studija

Matos,

João,

Matos

et al. 2023

Vet. stn. (Online)

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Nocardiosis is an emerging zoonosis caused by Nocardia spp. bacteria. The disease is linked to infections in immunocompromised individuals, and is potentially lethal when it turns systemic and is left untreated. The development of reliable and valid diagnostic methods is crucial to the identification of these pathogenic agents, particularly in wild mammals, which are potential reservoirs. The aim of this study was to determine the molecular prevalence of Nocardia spp. in wild mammals in Portugal. A sample of 114 wild mammals belonging to the orders Canidae, Cervidae, Erinaceidae, Herpestidae, Leporidae, Mustelidae, Viverridae, and Suidae were studied. Tissue samples (n=206) were collected from different organs. The molecular prevalence of Nocardia in wild mammals was 55.3% (95% CI: 45.7-64.6%). Nocardia spp. were detected in nine of ten species studied: 83.3% (95% CI: 62.2-100%) in Egyptian mongoose (Herpestes ichneumon), 63.2% (95% CI: 41.5-89.9%) in red fox (Vulpes vulpes), 45.9% (95% CI: 29.8-62.0%) in red deer (Cervus elaphus), 44.4% (95% CI: 28.2-60.6%) in wild boar (Sus scrofa), 3/3 beech martens (Martes foina), 2/2 Eurasian otters (Lutra lutra), 1/1 European badger (Meles meles), 1/1 genet (Genetta genetta), and 1/2 hedgehog (Erinaceus europeaeus). Of the 206 tissue samples studied by molecular techniques, Nocardia spp. were detected in 37.4% (95% CI: 36.7-38.1%), with a predominance in mesenteric lymph nodes (13.1%; 95% CI: 12.4-13.9%) and kidneys (9.2%; 95% CI: 8.5-9.9%). These results provide new insight into the prevalence of Nocardia in wild mammals and highlight the need for surveillance of wildlife as a potential reservoir of these emergent pathogens.

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Section: Discussionmentioning

confidence: 56%

Molekularni probir Nocardia spp. u divljih sisavaca - presječna studija

Matos,

João,

Matos

et al. 2023

Vet. stn. (Online)

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“… [19] 12 2022 49/M Lung Cough, hemoptysis 2 months TMP-SMZ, linezolid/ceftriaxone/minocycline Li et al. [20] CNS, central nervous system; F, female; M, male; TMP-SMX, trimethoprim-sulfamethoxazole. …”

Section: Discussionmentioning

confidence: 99%

Persistent Nocardia beijingensis infection in a patient with postoperative abscess and misuse of antibiotics in China

Qi,

Fan,

et al. 2023

Infectious Medicine

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“…With these technical advances have come increasing case reports and series identifying unusual or unexpected pathogens [22][23][24][25][26][27][28], comparative evaluations [29][30][31] and literature reviews [32 & ,33] demonstrating the value of RMg in a realworld adult and paediatric setting. Many were retrospective or observational, but some informed treatment in real time [34] and the protocol for a randomised multicentre clinical trial has been published [35 & ].…”

Section: The Respiratory Metagenomics Propositionmentioning

confidence: 99%

Respiratory metagenomics: route to routine service

Edgeworth

2023

Current Opinion in Infectious Diseases

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Purpose of reviewThe coronavirus disease 2019 pandemic demonstrated broad utility of pathogen sequencing with rapid methodological progress alongside global distribution of sequencing infrastructure. This review considers implications for now moving clinical metagenomics into routine service, with respiratory metagenomics as the exemplar use-case.Recent findingsRespiratory metagenomic workflows have completed proof-of-concept, providing organism identification and many genotypic antimicrobial resistance determinants from clinical samples in <6 h. This enables rapid escalation or de-escalation of empiric therapy for patient benefit and reducing selection of antimicrobial resistance, with genomic-typing available in the same time-frame. Attention is now focussed on demonstrating clinical, health-economic, accreditation, and regulatory requirements. More fundamentally, pathogen sequencing challenges the traditional culture-orientated time frame of microbiology laboratories, which through automation and centralisation risks becoming increasingly separated from the clinical setting. It presents an alternative future where infection experts are brought together around a single genetic output in an acute timeframe, aligning the microbiology target operating model with the wider human genomic and digital strategy.SummaryPathogen sequencing is a transformational proposition for microbiology laboratories and their infectious diseases, infection control, and public health partners. Healthcare systems that link output from routine clinical metagenomic sequencing, with pandemic and antimicrobial resistance surveillance, will create valuable tools for protecting their population against future infectious diseases threats.

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Successful treatment of disseminated nocardiosis diagnosed by metagenomic next-generation sequencing: A case report and review of literature

Cited by 5 publications

References 36 publications

Molekularni probir Nocardia spp. u divljih sisavaca - presječna studija

Molekularni probir Nocardia spp. u divljih sisavaca - presječna studija

Persistent Nocardia beijingensis infection in a patient with postoperative abscess and misuse of antibiotics in China

Respiratory metagenomics: route to routine service

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