Successful Sub-zero Non-freezing Preservation of Rat Lungs at −2°C Utilizing a New Supercooling Technology

Okamoto, Toshihiro; Nakamura, Takayuki; Zhang, Jitian; Aoyama, Akihiro; Chen, Fengshi; Fujinaga, Takuji; Tanaka, Shōji; Hamakawa, Hiroshi; Sakai, Hiroaki; Manabe, Toshiaki; Wada, Hiromi; Date, Hiroshi; Bando, Toru

doi:10.1016/j.healun.2008.07.008

Cited by 27 publications

(11 citation statements)

References 35 publications

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“…In previous reports, several experiments examined whether or not a supercooled condition is useful for preserving rat organs [1]–[3]. Takahashi et al [1] reported that when livers were preserved under a supercooled condition of −2°C with 5MPa pressure added, the shape of the tissue was maintained and the success rate of liver transplantation improved.…”

Section: Discussionmentioning

confidence: 99%

“…However, when the temperature of water is gradually lowered using a program freezer, etc., a “supercooling phenomenon” occurs in which the water does not change phase from liquid to solid even when the temperature falls below 0°C. Furthermore, it has been clarified that this supercooling phenomenon is stably caused by artificially applying pressure and voltage to water [1]–[3]. We recently invented a program freezer that causes supercooling conditions using a magnetic field.…”

Section: Introductionmentioning

confidence: 99%

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Effect of a Magnetic Field on Drosophila under Supercooled Conditions

et al. 2012

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Under subzero degree conditions, free water contained in biological cells tends to freeze and then most living things die due to low temperatures. We examined the effect of a variable magnetic field on Drosophila under supercooled conditions (a state in which freezing is not caused even below the freezing point). Under such supercooled conditions with the magnetic field at 0°C for 72 hours, −4°C for 24 hours and −8°C for 1 hour, the Drosophila all survived, while all conversely died under the supercooled conditions without the magnetic field. This result indicates a possibility that the magnetic field can reduce cell damage caused due to low temperatures in living things.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of a Magnetic Field on Drosophila under Supercooled Conditions

et al. 2012

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“…According to Okamoto et al, 21 when 3000 V is applied in preservation of a rat lung at a temperature of −2°C, reperfusion injury clearly declines compared to preservation at 4°C. Naito et al 22 reported that all Drosophilia survived under supercooled conditions with a magnetic field at 0°C for 72 hours, −4°C for 24 hours, and −8°C for 1 hour; in contrast, all died without the magnetic field.…”

Section: Discussionmentioning

confidence: 99%

Subzero 12-hour Nonfreezing Cryopreservation of Porcine Heart in a Variable Magnetic Field

Seguchi

Watanabe

Kato

et al. 2015

Transplantation Direct

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“…Slowing down metabolism and reducing high ATP consumption appears to be a key mechanism of cold storage and appears to be improved during supercooling. Preservation of organ energy status during supercooling has been confirmed in lungs (57) and hearts (58,59), where short-term supercooling preservation has been successfully performed, but transplantation has not been attempted.…”

Section: Supercoolingmentioning

confidence: 99%

Subzero organ preservation

Bruinsma

Uygun

2017

Current Opinion in Organ Transplantation

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Purpose of review Herein, we review the field of subzero organ preservation with a focus on recent developments in hepatic supercooling. Recent findings Organ preservation is making a rapid shift from the decade-old standard of storage on ice towards techniques that improve organ availability as well as preservation time. Long-term organ preservation would have tremendous benefits to the organ transplantation field, including better organ allocation, donor-recipient matching, as well as reduced preservation injury and subsequent improvement of donor organ use. The formation of ice has proven an important limiting factor and novel techniques attempt to control or prevent freezing using cryoprotective agents, and highly-controlled cooling regimens. Various techniques have been employed over the previous decades including true organ freezing, vitrification and subzero non-freezing, or supercooling. For most techniques, successful transplantation following long-term subzero preservation has remained elusive. Supercooling however recently delivered the first promising results, yielding survival after up to 4 days of supercooled preservation at −6 °C. Summary As the field of organ preservation undergoes significant development, the field of subzero preservation also receives renewed interest. While many obstacles remain to be overcome to make subzero preservation feasible, novel techniques are beginning to show their potential in achieving long-term preservation.

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Successful Sub-zero Non-freezing Preservation of Rat Lungs at −2°C Utilizing a New Supercooling Technology

Cited by 27 publications

References 35 publications

Effect of a Magnetic Field on Drosophila under Supercooled Conditions

Effect of a Magnetic Field on Drosophila under Supercooled Conditions

Subzero 12-hour Nonfreezing Cryopreservation of Porcine Heart in a Variable Magnetic Field

Subzero organ preservation

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