Successful Rescue of Wild Trametes versicolor Strains Using Sawdust and Rice Husk-based Substrate

Nguyen, Bich Thuy; Le, Ve Van; Nguyen, Hoai-Son; Nguyen, Luyen Thi; Tran, Anh Dong; Ngo, Nghien Xuan

doi:10.3923/pjbs.2021.374.382

Cited by 2 publications

(11 citation statements)

References 24 publications

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“…The nutrition requirements for the mycelium growth of Lingzhi mushrooms primarily include carbon and nitrogen resources (Zhou et al, 2012 ). Carbon sources, which act as structural and storage compounds in the cell, should be optimized first among the constituents of nutritional compositions (Nguyen et al, 2021 ). G. lucidum can use a wide range of carbon sources, including dextrin, galactose, mannose, maltose, sucrose, and xylose (Jayasinghe et al, 2008 ).…”

Section: Resultsmentioning

confidence: 99%

“…In this study, sawdust was used as a basal substrate. Since sawdust has a low nutrient content and may be insufficient for the development of fruiting bodies, it was enriched with wheat bran, which acts as a nitrogen reservoir for mushrooms (Nguyen et al, 2021 ). However, high supplementation with wheat bran can increase the risk of contamination, inhibit the mycelial growth, and reduce the yield of mushrooms (Nguyen et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

“…GA21) was deposited at the Mushroom Research and Development Center, Vietnam. Pure mycelial cultures were isolated from healthy fruiting bodies using a previously described tissue culture technique (Nguyen et al, 2021 ). The inner tissue (approximately 3 cm) of the fruiting body was inoculated in a PDA medium in a Petri dish at 25°C for 7 days in a dark room.…”

Section: Methodsmentioning

confidence: 99%

“…The cultivation of wild strains before their extinction is considered a realistic approach to overcome this challenge. Previous studies have reported the successful cultivation of several wild mushroom strains, such as Lepista sordida (Thongbai et al, 2017 ), Trametes versicolor (Nguyen et al, 2021 ), and Pycnoporus sanguineus (Dulay and Damaso, 2020 ). However, to the best of our knowledge, no study has evaluated the potential cultivation of wild G. sinense strains.…”

Section: Introductionmentioning

confidence: 99%

“…Culture conditions are the primary factors affecting the mycelial growth and fruiting body formation in mushrooms (Nguyen et al, 2021 ). Among Ganoderma species, the majority of the studies focused on identifying the culture conditions for the mycelial growth of Ganoderma lucidum (Jayasinghe et al, 2008 ) and Ganoderma applanatum (Jo et al, 2009 ), but not for other species.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of mycelial growth and cultivation of wild Ganoderma sinense

Nguyen¹,

Le²,

Nguyen³

et al. 2023

bta

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Ganoderma sinense, a well-known medicinal macrofungus of Basidiomycetes, is widely used in traditional medicine for promoting health and longevity in East Asia. The fruiting bodies of G. sinense contain polysaccharides, ergosterol, and coumarin, which have antitumor, antioxidant, and anticytopenia activities. Mushroom cultivation requires suitable conditions for the formation of fruiting bodies and yield. However, little is known about the optimal culture conditions for mycelial growth and cultivation of G. sinense. In this study, the successful cultivation of a G. sinense strain collected from the wild was reported. The optimal culture conditions were identified by examining one factor at a time. The results of this study revealed that the nutritional requirements for the optimal mycelial growth of G. sinense were fructose (15 g/l) as the carbon source and yeast extract (1 g/l) as the nitrogen source. The optimal pH and temperature for G. sinense were 7 and 25-30EC, respectively. The mycelia grew fastest in treatment II (69% rice grains + 30% sawdust + 1% calcium carbonate). G. sinense produced fruiting bodies under all tested conditions and showed the highest biological efficiency (2.95%) in treatment B (96% sawdust, 1% wheat bran, 1% lime). In summary, under optimal culture conditions, G. sinense strain GA21 showed satisfactory yield and a high potential for commercial cultivation.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optimization of mycelial growth and cultivation of wild Ganoderma sinense

Nguyen¹,

Le²,

Nguyen³

et al. 2023

bta

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Cultural and Morphological Features of Trametes versicolor (Polyporaceae) Growth on Wood Hydrolyzes Containing Media

Zubyk,

Klechak

2023

Innov Biosyst Bioeng

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Background. Trametes versicolor is a representative of basidiomycota, whose biologically active compounds are used in medicine and industry, so the search for active producers capable of growing on industrial waste and the study of new ways of intensification of growth and synthesis of metabolites are relevant task. Objective. Investigation of the effect of Fagales sawdust extracts on growth, morphological characteristics, and induction of synthesis of oxidizing enzymes by macromycetes of the species T. versicolor in surface culture. Methods. The objects of research were 5 strains of T. versicolor, which were cultivated on the synthetic agarized Norcrans media on Petri dishes. The influence of the sawdust extracts of 3 tree species, which acted as a basis for research media, was studied. The following cultural indicators were investigated: colony size, radial growth rate, and growth rate. The research also covered the study of macromycetes' morphological characteristics and the ability of Fagales sawdust extracts to induce the synthesis of oxidative enzymes. Results. It is established that growth on media with the addition of birch extract is more intense: the radial growth rate is 11.2–13.6 mm/day, while for beech and oak, the growth rate ranges from 11.6–13.1 and 11.5–12.6 mm/day respectively. The highest growth value on all media is recorded for T. versicolor 353. The value of the growth rate on the medium with birch sawdust extracts varied in the range 15.8–92.1, for medium with beech extracts – 19.4–46.1, with oak extracts – 15.5–46,7, and the highest growth value was recorded during the cultivation of T. versicolor 5299. The beech and oak sawdust extracts intensified the synthesis of laccase, peroxidase, and tyrosinase, which was strongly expressed in T. versicolor 353, so it was chosen for further experiments with submerged cultivation. Conclusions. Birch, beech, and oak sawdust extracts can be used to increase the accumulation of T. versicolor biomass while beech and oak sawdust extracts are preferable for the intensification of the synthesis of oxidase-type enzymes.

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Successful Rescue of Wild Trametes versicolor Strains Using Sawdust and Rice Husk-based Substrate

Cited by 2 publications

References 24 publications

Optimization of mycelial growth and cultivation of wild Ganoderma sinense

Optimization of mycelial growth and cultivation of wild Ganoderma sinense

Cultural and Morphological Features of Trametes versicolor (Polyporaceae) Growth on Wood Hydrolyzes Containing Media

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