Successful Repair of a Critical-Sized Bone Defect in the Rat Femur with a Newly Developed External Fixator

Zhao, Zhenyu; Yang, Dae Suk; Ma, Xu; Zhao, Hongmei; Nie, Chunlei; Si, Zhenxing

doi:10.1620/tjem.219.115

Cited by 27 publications

(15 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to characterize the induced membrane and assess the cellular and molecular mechanisms involved in large bone defect reconstruction, an experimental model in the rat that mimics the Masquelet technique was developed. Technically, resections smaller than 6 mm should not be considered for tissue engineering techniques designed to achieve bone union because of the spontaneous healing capacities observed in the rat (Drosse et al ., ; Zhao et al ., ). In the present study, bone consolidation of an 8 mm defect was obtained within 18 weeks by using the Masquelet technique, while the absence of spontaneous consolidation of this resection has already been demonstrated (Oakes et al ., ).…”

Section: Discussionmentioning

confidence: 54%

Osteoclasts and their precursors are present in the induced-membrane during bone reconstruction using the Masquelet technique

Gouron

Petit

Boudot

et al. 2014

J Tissue Eng Regen Med

View full text Add to dashboard Cite

In 2000, Masquelet reported a long bone reconstruction technique using an induced membrane formed around a polymethylmethacrylate (PMMA) spacer placed in the defect with appropriate stabilization followed by secondary bone graft after PMMA removal. This reconstruction procedure allows rapid and safe bone reformation for septic, traumatic, neoplastic or congenital bone defects. A rat model of the Masquelet technique was developed to further characterize the biological activities of this induced membrane. Our model allows healing of a critical-sized femoral defect (8 mm) by means of this procedure over a period of 18 weeks. Comparison of induced membranes obtained 3, 4, 5 and 6 weeks after PMMA insertion indicated that this tissue changes over time. Several mineralization spots and bone cells were observed in contact with the PMMA, when assessed by Alizarin Red, Von Kossa, Alkaline phosphatase and Tartrate-resistant acid phosphatase staining of the membranes. CTR (calcitonin receptor)- and RANK (Receptor Activator of Nuclear factor Kappa B)- positive mononuclear cells were detected in the induced membrane, confirming the presence of osteoclasts in this tissue. These cells were observed in a thin, highly cellular layer in the induced membrane in contact with the PMMA. Together, these findings suggest that the membrane is able to promote osteointegration of autologous corticocancellous bone grafts during the Masquelet technique by creating local conditions that may be favourable to graft bone remodelling and osteointegration. Copyright © 2014 John Wiley & Sons, Ltd.

show abstract

Section: Discussionmentioning

confidence: 54%

Osteoclasts and their precursors are present in the induced-membrane during bone reconstruction using the Masquelet technique

Gouron

Petit

Boudot

et al. 2014

J Tissue Eng Regen Med

View full text Add to dashboard Cite

show abstract

“…Therefore, a purely synthetic graft is an attractive alternative, but like an allograft, it would need to be supplemented by an osteoinductive agent. Animal models utilizing plates, external fixators, or nails can be used to study segmental bone defects . A bone critical size defect was originally defined by Schmitz and Hollinger in 1986 as “the smallest size intra osseous wound in a particular bone and species of animal that will not heal spontaneously during the lifetime of the animal.” A 6 mm femoral defect in the rat has been shown to represent a critical size defect .…”

mentioning

confidence: 99%

The masquelet induced membrane technique with BMP and a synthetic scaffold can heal a rat femoral critical size defect

Bosemark

Perdikouri

Pelkonen

et al. 2015

Journal Orthopaedic Research

View full text Add to dashboard Cite

Long bone defects can be managed by the induced membrane technique together with autologous bone graft. However, graft harvest is associated with donor site morbidity. This study investigates if a tricalcium phosphate hydroxyapatite scaffold can be used alone or in combination with bone active drugs to improve healing. Sprague Dawley rats (n ¼ 40) were randomized into four groups. (A) scaffold, (B) BMP-7, (C) BMP-7 þ scaffold, and (D) BMP-7 þ scaffold þ systemic bisphosphonate at 2 weeks. Locked femoral nailing was followed by 6 mm segment removal and implantation of an epoxy spacer. At 4 weeks, the spacers were removed and the defects grafted. Eleven weeks later, the bones were explanted for evaluation with radiography, manual assessment, micro-CT, histology, and Fourier Transform Infrared spectroscopy (FTIR). Isolated scaffolds (A) did not heal any defects, whereas the other treatments led to healing in 7/10 (B), 10/10 (C), and 9/10 (D) rats. Group D had greater volume of highly mineralized bone (p < 0.01) and higher bone volume fraction (p < 0.01) compared to all other groups. A synthetic scaffold þ BMP-7 combined with a bisphosphonate improved the callus properties in a rat femoral critical size defect, compared to both BMP-7 and scaffold alone or the two combined.

show abstract

“…A rat femoral model was used in this research since this model was reported to be capable of supporting weight‐bearing femoral defects 31 and have been used frequently in orthopedic tissue engineering studies 32 . This model has been reported for easy handling 33 and given the bone matrix remodeling rate in rats, the studies were relatively shorter compared to large animal models while providing highly valuable data in terms of therapeutic safety and feasibility during 2 months of the study 33,34 .…”

Section: Methodsmentioning

confidence: 99%

Regeneration enhanced in critical‐sized bone defects using bone‐specific extracellular matrix protein

et al. 2020

View full text Add to dashboard Cite

Extracellular matrix (ECM) products have the potential to improve cellular attachment and promote tissue‐specific development by mimicking the native cellular niche. In this study, the therapeutic efficacy of an ECM substratum produced by bone marrow stem cells (BM‐MSCs) to promote bone regeneration in vitro and in vivo were evaluated. Fluorescence‐activated cell sorting analysis and phenotypic expression were employed to characterize the in vitro BM‐MSC response to bone marrow specific ECM (BM‐ECM). BM‐ECM encouraged cell proliferation and stemness maintenance. The efficacy of BM‐ECM as an adjuvant in promoting bone regeneration was evaluated in an orthotopic, segmental critical‐sized bone defect in the rat femur over 8 weeks. The groups evaluated were either untreated (negative control); packed with calcium phosphate granules or granules+BM‐ECM free protein and stabilized by collagenous membrane. Bone regeneration in vivo was analyzed using microcomputed tomography and histology. in vivo results demonstrated improvements in mineralization, osteogenesis, and tissue infiltration (114 ± 15% increase) in the BM‐ECM complex group from 4 to 8 weeks compared to mineral granules only (45 ± 21% increase). Histological observations suggested direct apposition of early bone after 4 weeks and mineral consolidation after 8 weeks implantation for the group supplemented with BM‐ECM. Significant osteoid formation and greater functional bone formation (polar moment of inertia was 71 ± 0.2 mm4 with BM‐ECM supplementation compared to 48 ± 0.2 mm4 in untreated defects) validated in vivo indicated support of osteoconductivity and increased defect site cellularity. In conclusion, these results suggest that BM‐ECM free protein is potentially a therapeutic supplement for stemness maintenance and sustaining osteogenesis.

show abstract

Successful Repair of a Critical-Sized Bone Defect in the Rat Femur with a Newly Developed External Fixator

Cited by 27 publications

References 25 publications

Osteoclasts and their precursors are present in the induced-membrane during bone reconstruction using the Masquelet technique

Osteoclasts and their precursors are present in the induced-membrane during bone reconstruction using the Masquelet technique

The masquelet induced membrane technique with BMP and a synthetic scaffold can heal a rat femoral critical size defect

Regeneration enhanced in critical‐sized bone defects using bone‐specific extracellular matrix protein

Contact Info

Product

Resources

About