Successful reinitiation and restoration of spermatogenesis in hypophysectomized rats with pregnant mare's serum after a long-term regression period

Berswordt-Wallrabe, R. von; Neumann, F.

doi:10.1007/bf02144417

Cited by 15 publications

(5 citation statements)

References 4 publications

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“…This is quite in contrast to previous findings in the rat, in which neither hypophysectomy and FSH replacement (Clermont & Harvey 1967;von Berswoldt-Wallrabe 8c Neumann 1968) nor FSH antiserum administration (Dym et al 1979;Lee et al 1978) resulted in any effect on the testis. In rats it is possible to maintain spermatogenesis following hypophysectomy by testosterone administration (Ahmad et al 1973), whereas in hypogonadotrophic men, once spermatogenesis has been initiated with hCG and FSH, it cannot be maintained by testosterone alone, indicating the necessity for another factor (Mancini et al 1968).…”

Section: Discussioncontrasting

confidence: 84%

The role of follicle stimulating hormone in testicular function of the mature rhesus monkey

Wickings

Usadel

Dathe

et al. 1980

Acta Endocrinologica

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In the rat, whilst FSH has been shown to be necessary for the initiation of spermatogenesis, this hormone does not appear to be involved in the maintenance of spermatogenesis in the mature animals. Since the role of FSH has not been conclusively established in primate species, including humans, a study based on the neutralization of FSH by passive immunization with specific FSH antibodies was initiated in adult male rhesus monkeys.Prior to the actual study an antiserum pool was generated from 4 monkeys actively immunized with highly purified ovine FSH. In this FSH antiserum there was no evidence for the existence of LH antibodies, and the capacity of the antiserum to neutralize rhesus FSH was demonstrated by bioassay. Because of marked circannual variations in testicular functions passive immunization with FSH-antiserum was performed during the period of full testicular development in 4 other monkeys. FSH antiserum was injected for 106 days (i.e. a period covering the full duration of the total spermatogenetic process twice). Four further monkeys serving as controls were injected with normal rhesus serum for the same period. Testicular volume and histology, anti-FSH-titer, seminal parameters, serum testosterone, and LH were monitored at regular intervals. In the passively immunized animals the testicular volume decreased by 50%, sperm counts decreased below control counts, histology revealed reduction of the tubular diameter by 40%, derangement of spermatogenesis and, on instances, tubuli with Sertoli cells only. Leydig cells appeared histologically normal, although testosterone levels in the passively immunized monkeys tended to be lower than in control animals. Values were not significantly different at the end of the observation period.From these results it can be concluded that in contrast to the rat FSH plays a specific role in the maintenance of spermatogenesis in the mature rhesus monkey.

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Section: Discussioncontrasting

confidence: 84%

The role of follicle stimulating hormone in testicular function of the mature rhesus monkey

Wickings

Usadel

Dathe

et al. 1980

Acta Endocrinologica

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“…After 45 days of eCG administration, matured spermatozoa appeared in the epididymides. These results agreed with previous observations in hypophysectomized animals [14]. The duration for these processes and the composition of the germ cells in hpg mice were quite similar to those in normal mice [15], although the response to hormone therapy of the hpg mice was much weaker than that of hypophysectomized mice [5,16,17].…”

Section: Discussionsupporting

confidence: 91%

“…Many previous stuides [14,[18][19][20] have reported that eCG was effective for completing spermatogenesis, but single administration of GnRH did not induce whole spermatogenesis [5]. A daily injection of GnRH may have less stimulation to produce androgen, because weight of seminal vesicles after 60 days of GnRH administration in hpg mice was lower than that of mice given eCG for 60 days.…”

Section: Discussionmentioning

confidence: 99%

Effect of eCG Administration on the Reproductive Function in Hypogonadal (hpg) Male Mice.

Hashizume

Tsujii

Takagi

et al. 1996

Journal of Reproduction and Development

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“…Both qualitative and quantitative studies on the hormonal control of spermatogenesis have been made, and this may explain some of the conflicting conclusions which have been reached since the observations that FSH and LH control spermatogenesis (Greep et al 1936;Greep and Fevold 1937). Woods and Simpson (1961) and Lostroh (1963) found that neither FSH nor LH alone was able to restore spermatogenesis in the hypophysectomized rat, but FSH and LH together have been reported as necessary for restoration of spermatogenesis (Woods and Simpson 1961;Lostroh 1963;Von Berswordt-Wallrabe and Neumann 1968). Clermont and Harvey (1967) found that no hormonal combinations were able quantitatively to restore spermatogenesis in the rat.…”

Section: Discussionmentioning

confidence: 99%

Studies in Testicular Function. I. Quantitative Effects of FSH, LH, Testosterone and Dihydro-testosterone on Restoration and Maintenance of Spermatogenesis in the Hypophysectomized Rat

Js¹,

Aw²

1974

Aust. Jnl. Of Bio. Sci.

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Male Wistar rats were hypophysectomized and the effects on spermatogenesis were quantified by stereological techniques. Restoration of normal spermatogenesis was attempted 32 days after hypo-physectomy by treatment with FSH (designated F), LH (L) or testosterone (T) singly or in combina-tion for a further 32 days. In another experiment, maintenance of spermatogenesis was attempted using FSH (F), testosterone (T) or dihydrotestoterone (D). Treatment began two days after hypo-physectomy and was continued for a further 32 days.

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Successful reinitiation and restoration of spermatogenesis in hypophysectomized rats with pregnant mare's serum after a long-term regression period

Cited by 15 publications

References 4 publications

The role of follicle stimulating hormone in testicular function of the mature rhesus monkey

The role of follicle stimulating hormone in testicular function of the mature rhesus monkey

Effect of eCG Administration on the Reproductive Function in Hypogonadal (hpg) Male Mice.

Studies in Testicular Function. I. Quantitative Effects of FSH, LH, Testosterone and Dihydro-testosterone on Restoration and Maintenance of Spermatogenesis in the Hypophysectomized Rat

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