Successful preparation and characterization of biotechnological grade agarose from indigenous Gelidium amansii of Taiwan

Wang, Tzu-Pin; Chang, Lin‐Li; Chang, Sheng Nan; Wang, Eng Chi; Hwang, Long Chih; Chen, Yen Hsu; Wang, Yun-Ming

doi:10.1016/j.procbio.2011.12.015

Cited by 28 publications

(17 citation statements)

References 21 publications

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“…For electrophoresis of nucleic acids, agarose is usually purified from agar by removing agaropectin via washing with EDTA, isopropanol, Al(OH) 3 , or DMSO [23,24,47,48], which involves complicated processes and costs. An important property of agarose is its low amount of sulfate in comparison with agar [23]. As mentioned above, sulfate contents in the P. yezoensis agar are very low (Fig.…”

Section: Suitability Of P Yezoensis Agar For Electrophoresis Of Dnamentioning

confidence: 99%

“…The capacity of agar to gel, with concomitant high water absorption, contributed to the epoch-making advance of using agar to solidify media in plates for bacterial culture [2][3]. Although not all red seaweeds contain agar, Gracilariaceae including Gracilaria, Curdiea, and Hydropuntia [4][5][6][7][8][9][10][11][12][13][14][15][16][17], as well as Gelidium and Pterocladia of the Gelideales [6,[18][19][20][21][22][23][24] are major agar sources [25]. Agar from these seaweeds represents complex polysaccharide structures consisting of agarose and agaropectin [1].…”

Section: Introductionmentioning

confidence: 99%

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Optimization of yield and quality of agar polysaccharide isolated from the marine red macroalga Pyropia yezoensis

et al. 2017

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The marine red seaweed Pyropia yezoensis is grown on a large scale in Japan using mariculture for production of nori sheets. Here, we optimized the isolation of agar from P. yezoensis and evaluated its yield, physical properties, and product applications. An alkali pre-treatment of seaweeds before agar extraction increased the weight-average molecular weight of the agar and promoted the conversion of L-galactose sulfate to 3,6-anhydrogalactose, which in turn reduced the sulfate contents, thus altering the gel strength of the agar. This ability to adjust agar quality by altering the alkali concentration during material pre-treatment allowed production of agar with properties similar to those of agar from the red seaweed Gelidium sp., which is widely used for industrial agar applications. We demonstrate the suitability of P. yezoensis agar in production of solid plates for bacterial growth. In addition, the P. yezoensis agar was particularly useful as a gel material, with the capacity for excellent size separation of DNA by electrophoresis even without agarose purification, because of quite low contents of sulfate. These findings support the applicability and usefulness of P. yezoensis for agar production. The established large-scale cultivation methods for P. yezoensis can generate sufficient biomass to produce agar to support medical and biological studies.

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Section: Suitability Of P Yezoensis Agar For Electrophoresis Of Dnamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of yield and quality of agar polysaccharide isolated from the marine red macroalga Pyropia yezoensis

et al. 2017

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“…4). 53,54 Carrageenan is a generic name for a family of polysaccharides that includes six basic forms: iota-, kappa-, lambda-, in 0.9 N sulfuric acid and hydrolysate was filtered and used again for a second hydrolysis. In the second hydrolysis, new seaweed was hydrolyzed in the filtrate of the hydrolysate prepared by the first hydrolysis.…”

Section: Production Of Sugars and Ethanol From Red Seaweedsmentioning

confidence: 99%

“…The structure of the repeating disaccharide units in agarose and agaropectin 54. The repeating disaccharide unit in agarose consists of β-d-galactose and 3,6-anhydro-α-l-galactose (…”

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confidence: 99%

Strategies for the production of high concentrations of bioethanol from seaweeds

2013

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Bioethanol has attracted attention as an alternative to petroleum-derived fuel. Seaweeds have been proposed as some of the most promising raw materials for bioethanol production because they have several advantages over lignocellulosic biomass. However, because seaweeds contain low contents of glucans, i.e., polysaccharides composed of glucose, the conversion of only the glucans from seaweed is not sufficient to produce high concentrations of ethanol. Therefore, it is also necessary to produce ethanol from other specific carbohydrate components of seaweeds, including sulfated polysaccharides, mannitol, alginate, agar and carrageenan. This review summarizes the current state of research on the production of ethanol from seaweed carbohydrates for which the conversion of carbohydrates to sugars is a key step and makes comparisons with the production of ethanol from lignocellulosic biomass. This review provides valuable information necessary for the production of high concentrations of ethanol from seaweeds.

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“…The structural differences between agarose and agaropectin are indicated by substitutions with various functional groups which polymerize into a long chain as Fig. 1 [5].This situation has interrupted the molecular forces of hydrogen bonds, hence weakening the semi crystalline structure of materials. Besides, the motional freedom of polysaccharides chains in amorphous regions also increases due to poor inter and intra molecular reaction of hydrogen bonds.…”

Section: Introductionmentioning

confidence: 99%

Fourier Transform Infrared Spectroscopy of Porous Zinc with Different Concentration of Potassium Hydroxidein Agar Binder

Zamri¹,

Masri²

2018

IJCRSET

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Fourier Transform Infrared (FTIR) is a method to identify the chemical bonding exists in materials. FTIR on porous Zn have been conducted with different concentration of potassium hydroxide solution. Bacto-agar and commercial agar were applied as binder in porous Zn. Both agars show the typical spectra of agar-like galactans under FTIR. The peaks in the range of 400-600 cm-1 were attributed to the Zn molecules. Applying different concentration of KOH on porous Zn gave different intensities to the spectra of FTIR for both agars. As KOH concentration increased, the amount of OHincreased resulting the increasing of peak belong to OHand Zn bonding. Besides, addition of Zn and KOH in agar might not affect the bare Zn.

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Successful preparation and characterization of biotechnological grade agarose from indigenous Gelidium amansii of Taiwan

Cited by 28 publications

References 21 publications

Optimization of yield and quality of agar polysaccharide isolated from the marine red macroalga Pyropia yezoensis

Optimization of yield and quality of agar polysaccharide isolated from the marine red macroalga Pyropia yezoensis

Strategies for the production of high concentrations of bioethanol from seaweeds

Fourier Transform Infrared Spectroscopy of Porous Zinc with Different Concentration of Potassium Hydroxidein Agar Binder

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