Successful pregnancy following blastocyst cryopreservation using super-cooling ultra-rapid vitrification

Huang, Chun‐Chia; Lee, Tsung-Hsien; Chen, Shee‐Uan; Chen, Hsiu‐Hui; Cheng, Tzu‐Chun; Liu, Chung‐Hsien; Yang, Yu Shih; Lee, Maw‐Sheng

doi:10.1093/humrep/deh540

Cited by 97 publications

(52 citation statements)

References 30 publications

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“…An even higher post-thawing survival rate could be obtained by artificially collapsing blastocoels before vitrifying (Hiraoka et al, 2004). The consistently high postthawing survival rate and the similarity between vitrified embryos and nonvitrified embryos, in terms of their respective implantation rates and pregnancy rates, have been reported repeatedly (Mukaida et al, 2003;Hiraoka et al, 2004;Huang et al, 2005;Isachenko et al, 2007;Kartberg et al, 2008;Raju et al, 2009;Wennerholm et al, 2009).…”

Section: Human-assisted Reproductive Technologiesmentioning

confidence: 91%

Cryopreservation of mammalian embryos: Advancement of putting life on hold

Tsang

Chow

2010

Birth Defects Research Pt C

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Rodent transgenesis and human-assisted reproductive programs involve multistep handling of preimplantation embryos. The efficacy of production and quality of results from conventionally scheduled programs are limited by temporal constraints other than the quality and quantities of embryos per se. The emergence of vitrification, a water ice-free cryopreservation technique, as a reliable way to arrest further growth of preimplantation embryos, provides an option to eliminate the time constraint. In this article, current and potential applications of cryopreservation to facilitate laboratory animal experiments, colony management, and human-assisted reproductive programs are reviewed. Carrier devices developed for vitrification in the last two decades are compared with an emphasis on their physical properties that infer cooling rate of samples and sterility assurance. Biological impacts of improved cryopreservation on preimplantation embryos are also discussed.

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Section: Human-assisted Reproductive Technologiesmentioning

confidence: 91%

Cryopreservation of mammalian embryos: Advancement of putting life on hold

Tsang

Chow

2010

Birth Defects Research Pt C

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“…Until now, vitrification has been widely used for the cryopreservation of human oocytes [18,24,25], in vitro matured oocytes [26,27] pronuclear stage , cleavage stage [5,7,14,18,[29][30][31][32][33][34], or blastocyst-stage [8,18,[35][36][37][38][39][40][41][42]. However there are few publications that show clinical data on the basis of vitrification versus slow freezing, especially for the cleavage stage [5,8].…”

Section: Introductionmentioning

confidence: 99%

Vitrification versus slow freezing gives excellent survival, post warming embryo morphology and pregnancy outcomes for human cleaved embryos

Valojerdi

Eftekhari‐Yazdi

Karimian

et al. 2009

J Assist Reprod Genet

141

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Purpose The objective of this retrospective study was to evaluate the efficacy of vitrification and slow freezing for the cryopreservation of human cleavage stage embryos in terms of post-warming survival rate, post-warming embryo morphology and clinical outcomes. Methods The embryos of 305 patients at cleavage stages were cryopreserved either with vitrification (153 patients) or slow-freezing (152 patients) methods. After warming; the survival rate, post-warmed embryo morphology, clinical pregnancy and implantation rates were evaluated and compared between the two groups. Result(s) In the vitrification group versus slow freezing group, the survival rate (96.9% vs. 82.8%) and the postwarmed excellent morphology with all blastomeres intact (91.8% vs. 56.2%) were higher with an odds ratio of 6.607 (95% confidence interval; 4.184-10.434) and 8.769 (95% confidence interval; 6.460-11.904), respectively. In this group, the clinical pregnancy rate (40.5% vs. 21.4%) and the implantation rate (16.6% vs. 6.8%) were also higher with an odds ratio of 2.427 (95%confidence interval; 1.461-4.033) and 2.726 (95% confidence interval; 1.837-4.046), respectively. Conclusion(s) Vitrification in contrast to slow freezing is an efficient method for cryopreservation of human cleavage stage embryos. Vitrification provides a higher survival rate, minimal deleterious effects on post-warming embryo morphology and it can improve clinical outcomes.

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“…Stehlik et al [25] reported that the pregnancy rate was 16% and survival rate 83% in slow cooling protocol in comparison to vitrified blastocysts which gave 100% survival and 50% pregnancy rate. The pregnancy rate following vitrification observed by Huang et al [26] showed 53.8% and 77% survival rate. The largest study published till now comparing slow cooling protocol versus vitrification was performed on over than 6000 blastocysts by Kuwayama et al [27].…”

Section: Discussionmentioning

confidence: 92%

Vitrification vs. slow cooling protocol using embryos cryopreserved in the 5th or 6th day after oocyte retrieval and IVF outcomes.

Kuć

Kuczyńska²,

Stankiewicz³

et al. 2010

Folia Histochem Cytobiol.

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Modifying cryopreservation protocols may be seen as a way to simplify cryobanking procedure and increase satisfying outcomes. The aim of the study was to evaluate the influence of slow cooling protocol and vitrification on IVF outcomes using embryos preserved in the 5 th or 6 th day after oocyte retrieval. The study compared 2 groups of human embryos underwent slow cooling protocol (n=189) and vitrification (n=58). All embryos were cryopreserved in the 5 th or 6 th day after oocyte retrieval. Pre-and postfreezing embryo evaluation was performed in 2 or 3 steps scale, respectively. The study evaluates the effectiveness of two freezing methods and influence of the freezing day, pre-and postfreezing embryo grading on clinical pregnancy rate. Study showed higher pregnancy rate after vitrification (50.4%) than slow cooling protocol (25.9%). Significantly higher pregnancy rate was observed, when embryo preserved in the 5 th day after oocyte retrieval (50.3%) than in the 6 th day (22.7%). Postfreezing embryos evaluation showed that high quality blastocysts gave nearly four times better pregnancy outcomes than the ones evaluated as poor quality, and three times better than the ones evaluated as moderate. Prospective trials are needed to evaluate pregnancy and neonatal outcomes after vitrification. The number of controlled studies concerning vitrification has not been large enough, yet.

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Successful pregnancy following blastocyst cryopreservation using super-cooling ultra-rapid vitrification

Abstract: The ultra-rapid vitrification of blastocysts with a successful SR and PR could be used to replace classical slow cooling.

Cited by 97 publications

References 30 publications

Cryopreservation of mammalian embryos: Advancement of putting life on hold

Cryopreservation of mammalian embryos: Advancement of putting life on hold

Vitrification versus slow freezing gives excellent survival, post warming embryo morphology and pregnancy outcomes for human cleaved embryos

Vitrification vs. slow cooling protocol using embryos cryopreserved in the 5th or 6th day after oocyte retrieval and IVF outcomes.

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