Successful Pregnancy After the Vitrification of Zygotes Using Commercial Vitrification Solutions and Conventional Straws to Protect Against Infections in Liquid Nitrogen

Kumasako, Yoko; Kumon, Mami; Utsunomiya, Takafumi; Araki, Yasuhisa

doi:10.1007/s10815-005-0818-8

Cited by 10 publications

(5 citation statements)

References 14 publications

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“…The freezing and thawing procedure of the 2PN stage embryos was performed with the vitrification method; the vitrification and thawing kits (Kitazato BioPharma, Shizuoka, Japan) were used as previously described elsewhere (11). Briefly, the embryos were vitrified in a solution containing 15% ethylene glycol, 15% dimethyl sulfoxide, and 0.5 M sucrose using a 0.25-mL plastic straw.…”

Section: Methodsmentioning

confidence: 99%

The efficacy of the transfer of twice frozen-thawed embryos with the vitrification method

Kumasako¹,

Otsu²,

Utsunomiya³

et al. 2009

Fertility and Sterility

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Section: Methodsmentioning

confidence: 99%

The efficacy of the transfer of twice frozen-thawed embryos with the vitrification method

Kumasako¹,

Otsu²,

Utsunomiya³

et al. 2009

Fertility and Sterility

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“…Vitrification at the zygote stage is somewhat different. Unique cryopreservation techniques have been established by altering the concentration of CPA, cooling rate, and/or carrier devices (Kumasako et al, 2005;Park et al, 2000). Zygote vitrification is no longer experimental (Jelinkova et al, 2002) and has become a routine method in assisted reproduction treatment (Al-Hasani et al, 2007), with survival rates at least as high as with slow freezing.…”

Section: Clinical Outcomes Related To Zygote Morphology Before and After Cryopreservation With Slow Freezing And Vitrification (Thomas Ebmentioning

confidence: 99%

The Alpha consensus meeting on cryopreservation key performance indicators and benchmarks: proceedings of an expert meeting

Medicine¹

2012

Reproductive BioMedicine Online

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This proceedings report presents the outcomes from an international workshop designed to establish consensus on: definitions for key performance indicators (KPIs) for oocyte and embryo cryopreservation, using either slow freezing or vitrification; minimum performance level values for each KPI, representing basic competency; and aspirational benchmark values for each KPI, representing best practice goals. This report includes general presentations about current practice and factors for consideration in the development of KPIs. A total of 14 KPIs were recommended and benchmarks for each are presented. No recommendations were made regarding specific cryopreservation techniques or devices, or whether vitrification is 'better' than slow freezing, or vice versa, for any particular stage or application, as this was considered to be outside the scope of this workshop.

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“…Since then, vitrification has been widely used for the cryopreservation of human oocytes [4,5], in addition to pronuclear stage [6,7], cleavage stage [4,[8][9][10][11], and blastocyst-stage [4,[12][13][14] embryos. Human embryo vitrification has been attempted with a variety of vessels such as electron microscope grids [15,16], open pulled and hemi-straws [17], the Flexipet [18], the Cryotop [4] and the CryoLoop [19,20].…”

Section: Introductionmentioning

confidence: 99%

Vitrification of mouse embryos at 2-cell, 4-cell and 8-cell stages by cryotop method

Zhang

Cui

Ling

et al. 2009

J Assist Reprod Genet

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Purpose The objective of this study was to investigate the effects of vitrification on the preimplantation developmental competence of mouse 2-cell, 4-cell and 8-cell stage embryos. Methods Mouse 2-cell, 4-cell and 8-cell stage embryos were cryopreserved using the cryotop vitrification method and subsequently warmed on a later date. The embryos were then assessed by their morphology, blastocyst formation and hatching rates. Additionally, trophectoderm (TE) and inner cell mass (ICM) cell numbers were compared in hatched blastocysts from the control and experimental groups. Results Vitrified embryos at the 2-cell, 4-cell and 8-cell stages appeared morphologically normal after warming. The overall survival rate of vitrified embryos at various stages after warming was 96.7% and there were no significant differences among 2-cell stage (96.0%), 4-cell stage (96.8%) and 8-cell stage (97.1%) embryos (P>0.05). The blastocyst formation rate (69.4%) and hatching rate (52.6%) of vitrified 2-cell embryos were significantly lower than that from the control group and vitrified 8-cell embryos (P<0.05). In the vitrified 4-cell embryo group, the blastocyst formation rate (90.3%) was similar to the 8-cell group (91.2%), but the hatching rate (60.0%) was significantly lower than that of the non-vitrified control ( 84.1%) and vitrified 8-cell embryo (78.4%) groups (P<0.05). When further development to the fully hatched blastocyst stage was compared, hatched blastocysts derived from vitrified 2-cell, 4-cell and 8-cell embryos had significantly lower cell counts both in the ICM and TE, as compared to fresh blastocysts (P<0.05). Among the vitrified 2-cell, 4-cell and 8-cell embryo groups, there were no significant differences in the cell counts of ICM and TE (P>0.05). Conclusions Although cryotop vitrification was suitable for the cryopreservation of mouse embryos from the 2-cell stage, 4-cell stage and 8-cell stage without significant loss of survival, vitrification had an adverse effect on the development of 2-cell embryos. Mouse embryos at the 8-cell stage had the best tolerance for vitrification and would yield the highest level of post-vitrification developmental competence among early cleavage stage embryos. Nevertheless, it is unclear how these findings can be extrapolated to human embryos.

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Successful Pregnancy After the Vitrification of Zygotes Using Commercial Vitrification Solutions and Conventional Straws to Protect Against Infections in Liquid Nitrogen

Cited by 10 publications

References 14 publications

The efficacy of the transfer of twice frozen-thawed embryos with the vitrification method

The efficacy of the transfer of twice frozen-thawed embryos with the vitrification method

The Alpha consensus meeting on cryopreservation key performance indicators and benchmarks: proceedings of an expert meeting

Vitrification of mouse embryos at 2-cell, 4-cell and 8-cell stages by cryotop method

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