Successful pancreas preservation before islet isolation by the simplified two-layer cold storage method

Hiraoka, K.; Trexler, Alison M.; Eckman, E.; Stage, A.; Nevile, S.; Sageshima, Junichiro; Shibata, Satoshi; Sutherland, David E.R.; Hering, B. J.

doi:10.1016/s0041-1345(00)02281-8

Cited by 26 publications

(14 citation statements)

References 8 publications

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“…The high level of ␤-cell loss subsequent to isolation makes this number of islets difficult to obtain from one single pancreas, making until recently the use of "double-donor" transplants to provide for an adequate therapeutic islet mass a prerequisite to successful grafting (50). Better pancreas preservation before islet transplantation remains therefore one of the potential improvements to overcome islet shortage and to improve PNF (46,51).…”

Section: Discussionmentioning

confidence: 99%

Intracellular Stress Signaling Pathways Activated During Human Islet Preparation and Following Acute Cytokine Exposure

Abdelli¹,

Ansite

Roduit³

et al. 2004

Diabetes

169

146

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Pancreatic islet transplantation may successfully restore normoglycemia in type 1 diabetic patients. However, successful grafting requires transplantation of a sufficient number of islets, usually requiring two or more donors. During the isolation process and following clinical transplantation, islets are subjected to severe adverse conditions that impair survival and ultimately contribute to graft failure. Here, we have mapped the major intracellular stress-signaling pathways that may mediate human islet loss during isolation and following cytokine attack. We found that the isolation procedure potently recruits two pathways consisting of ͦmitogen-activated protein kinase kinase (MKK)7 3 Jun NH 2 -terminal kinase (JNK)/p38 3 c-fosͦ and the ͦnuclear factor-B (NF-B) 3 iNOSͦ module. Cytokines activate the ͦNF-B 3 iNOSͦ and ͦMKK4/MKK3/6 3 JNK/p38ͦ pathways without recruitment of c-fos. Culturing the islets for 48 h after isolation allows for the activated pathways to return to background levels, with expression of MKK7 becoming undetectable. These data indicate that isolation and cytokines recruit different death pathways. Therefore, strategies might be rationally developed to avoid possible synergistic activation of these pathways in mediating islet loss during isolation and following grafting.

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Section: Discussionmentioning

confidence: 99%

Intracellular Stress Signaling Pathways Activated During Human Islet Preparation and Following Acute Cytokine Exposure

Abdelli¹,

Ansite

Roduit³

et al. 2004

Diabetes

169

146

View full text Add to dashboard Cite

show abstract

“…Kidneys were stored in 20 ml of UW solution at 0-4 ° C for 24 h in the UW group and with a simplified TLM as described by Hiraoka et al [11] in the TLM group. PFC (perfluorodecalin, F2 Chemicals Limited, UK) was saturated with 100% oxygen 20 min before preservation.…”

Section: Preservation Methodsmentioning

confidence: 99%

“…Consequently, the TLM was reported to achieve better organ function in experimental pancreas [5,6] , heart [7] and intestinal transplantation [8] and to rescue the graft in a model of prolonged warm ischemia time [9] . Higher pancreatic islet yield and better function has been found by several research groups in comparison with conventional UW pancreas storage in experimental [10][11][12] , as well as in clinical studies [13,14] , though this effect has not been confirmed in a larger series [15][16][17] . The TLM for pancreas transplantation has therefore become a standard procedure in several centers supposedly enabling to prolong the cold ischemia time before islet isolation [18][19][20][21] .…”

Section: Introductionmentioning

confidence: 95%

Perfluorocarbon Improves Post-Transplant Survival and Early Kidney Function following Prolonged Cold Ischemia

Marada

Zacharovová²,

Saudek³

2010

Eur Surg Res

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Background: The two-layer organ preservation method (TLM) based on oxygenated perfluorocarbon overlaid with University of Wisconsin (UW) solution has been successfully used in clinical islet and experimental heart and intestine transplantation. We tested whether this technique would prevent tissue damage and improve kidney function in a model of syngeneic kidney transplantation with prolonged ischemia time. Methods: Kidneys were stored for 24 h either in UW solution (n = 16), with TLM (n = 16) or transplanted immediately (control group, n = 12). In half of the animals, survival was observed and in the other animals grafts were procured for semiquantitative histological scoring and TUNEL apoptosis assessment 24 h after transplantation. Results: One-month survival rates in the UW, TLM and control groups were 12.5, 62.5 and 100%, respectively (UW vs. TLM, p < 0.01). Median creatinine levels 24 h after transplantation were 381, 299 and 121 µM, respectively (UW vs. TLM, p < 0.02). Histological scoring showed more severe tissue damage in the UW group than in the TLM group (p < 0.05). Apoptosis was more frequent in the UW group than in the TLM group (p < 0.05). Conclusion: We demonstrated for the first time that conservation with TLM significantly improves the outcome of kidney transplantation in a rat model and should therefore be further studied in larger animals.

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“…While in the smaller trials pancreases had been subjected to continuous oxygenation for a short period of approximately 3 hours subsequent to cold storage (Tsujimura et al, 2002a, Tsujimura et al, 2004a, Salehi et al, 2006, the large scale trials utilized oxygen-precharged PFD for the entire period of cold ischemia. However, experiments in rats clearly suggested that oxygen-precharged PFD is equivalent to continuously oxygenated PFD with regard to pancreas ATP concentration and isolated islet yield after 24 hours of cold storage (Hiraoka et al, 2001). These findings were confirmed in human pancreases since no difference was observed regarding islet isolation outcome between the static and original TLM (Matsumoto et al, 2002b).…”

Section: Pancreas Oxygenation For Subsequent Islet Transplantationmentioning

confidence: 99%

Chemical Oxygenation of Pancreatic Tissue Prior to Islet Isolation and Transplantation

Brandhorst¹,

Johnson²,

Brandhorst³

2012

Organ Donation and Transplantation - Public Policy and Clinical Perspectives

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Successful pancreas preservation before islet isolation by the simplified two-layer cold storage method

Cited by 26 publications

References 8 publications

Intracellular Stress Signaling Pathways Activated During Human Islet Preparation and Following Acute Cytokine Exposure

Intracellular Stress Signaling Pathways Activated During Human Islet Preparation and Following Acute Cytokine Exposure

Perfluorocarbon Improves Post-Transplant Survival and Early Kidney Function following Prolonged Cold Ischemia

Chemical Oxygenation of Pancreatic Tissue Prior to Islet Isolation and Transplantation

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