Successful orthotopic uterine allotransplantation in a rabbit model using aorta and cava anastomoses: a short-term viability study

Liu, Yu; Zhang, Ying; Ding, Yan; Li, Junwei; Tang, Qin; Zhang, Xuyin; Hua, Keqin

doi:10.1007/s00404-019-05381-9

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…The surgical procedures were quite similar with our previously described 24 . However, the complicated posterior division of internal iliac vessels should be carefully ligated and transected after transecting the vaginal at 5–6 mm below the cervix.…”

Section: Methodsmentioning

confidence: 87%

Morphologic assessment of hypertonic citrate adenine, histidine–tryptophan–ketoglutarate, and university of Wisconsin solutions for hypothermic uterus preservation in rats

Ding

Zhang

et al. 2021

J of Obstet and Gynaecol

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Aim Optimizing perfusate for static cold storage is one of the key ways of reducing organ dysfunction and rejection in organ transplantation. Here, we tested the effectiveness of the three different solutions for hypothermic uterus preservation. Methods Twenty rats were divided into four groups, five in each group. Uterine grafts were retrieved and perfused in situ. The uteri were preserved at 4°C in normal saline as control group (group NS), hypertonic citrate adenine (group HCA), histidine–tryptophan–ketoglutarate (group HTK), or university of Wisconsin solutions (group UW) for 0, 12, 24, and 48 h, respectively. HE, electron microscopy, TUNEL staining, and Cleaved Caspase3 immunohistochemical staining were assessed at each time point. Results There was no significant difference in the uterine retrieval time, perfusion time, and the amount of perfusion solution in NS, HCA, HTK, and UW groups (p > 0.05). HCA and HTK can well preserve the pathological morphology of rat uterine tissues for up to 24 h, and the apoptosis rates of the two groups are 7.2% and 7.1%, respectively, with no statistical difference (p > 0.05). Still, the protective effect of HTK on the ultrastructure of cells was much better than HCA. There was a significant difference in the apoptosis rate of UW (6.5%), HTK (8.8%), and HCA (9.4%) at 48 h, with mitochondrial and endoplasmic reticulum structure well preserved only in UW. Conclusion At 4°C, normal saline is not suitable to preserve rat uterus for more than 12 h. The morphologic results would favor the use of HTK rather than HCA for short‐term hypothermic uterus preservation (≤24 h). UW is better than HTK and HCA for 48 h hypothermic uterus preservation.

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Section: Methodsmentioning

confidence: 87%

Morphologic assessment of hypertonic citrate adenine, histidine–tryptophan–ketoglutarate, and university of Wisconsin solutions for hypothermic uterus preservation in rats

Ding

Zhang

et al. 2021

J of Obstet and Gynaecol

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“…30,31 The modern era of human UTx studies was preceded by a thorough translational research program on laboratory animals. 2,30,[32][33][34][35] The animal UTx trials used mice, [36][37][38] rats, [39][40][41][42][43][44] rabbits, [45][46][47] pigs, [48][49][50][51][52] sheeps, 53-62 baboons [63][64][65][66][67] and cynomolgus monkeys. [68][69][70][71][72][73][74] Most of the UTx experiments on nonhuman primates were performed with autologous grafts.…”

Section: Experience Gained From Utx In Animal Experimental Studiesmentioning

confidence: 99%

Human uterine vasculature with respect to uterus transplantation: A comprehensive review

Křístek

Johannesson

Novotný

et al. 2020

J of Obstet and Gynaecol

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Due to the novelty of uterus transplantation, data on preferable inflow and outflow of the graft are limited. This paper reviews the technique, type of vessels and the outcome. A systematic search of the PubMed database was conducted. We extracted and analyzed data on the arteries and veins utilized, types of anastomosis, types of donors, complications and the outcome. Thirty eight sources reported 51 human uterine transplantations, 10 graft thromboses and 25 live births. Inflow was established with two uterine arteries (UA) with/without the anterior division of the internal iliac artery in 62% (n = 31) of cases, two UA arteries with a segment/patch of the internal iliac artery in 34% (n = 17) of cases or two UA with a conduit in 4% of cases (n = 2). Both cases with a conduit developed thrombosis (n = 2). Arterial thrombosis/ischemia developed in 8 of the 51 cases. In 50% of cases with arterial thrombosis, atherosclerosis was identified as a possible cause. Outflow was established by two internal iliac veins with patches/segments in 27.5% of cases (n = 14) followed by two utero-ovarian veins in 25.5% (n = 13). Venous thrombosis occurred in 3 of the 51 cases. Uterine arteries with/without anterior division of the internal iliac artery were the most frequent arteries used for inflow and produced the highest patency rate. The presence of atherosclerosis and complex arterial reconstruction was associated with a high rate of arterial thrombosis. None of the veins utilized in the procedures appeared to be superior. There are insufficient data to draw a definite conclusion.

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Application of modified sodium alginate hydrogel for interventional embolization of hemorrhagic diseases

Wei,

Deng,

et al. 2024

Front. Mater.

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Traditional particulate embolic agents are small in diameter, but can easily embolize the ends of blood vessels, resulting in ischemia and necrosis of normal tissues and organs. The metal spring embolic agent has a larger diameter, but it cannot be degraded and can easily cause permanent damage to blood vessels. Ideally, a bleeding embolism should achieve rapid hemostasis without causing long-term necrosis of organs and tissues. In this study, a modified sodium alginate hydrogel (MSAH) was prepared by mixing an oxidized sodium alginate (OSA) aqueous solution with a carboxymethyl chitosan (CMC) aqueous solution at a ratio of 1:6 in a 38°C bath for 8 min. The feasibility of this modified hydrogel was then tested in an internal iliac artery hemorrhage model using New Zealand rabbits. The MSAH had good adhesion. The hydrogel was injected through a single curved 4F catheter without obvious effects on uterine smooth muscle cell proliferation and apoptosis. The blood flow of the internal iliac artery was restored by long-term degradation of the sodium alginate hydrogel, and no ischemia and necrosis were observed by histopathology. The MSAH prepared using a mixture of OSA and CMC had good adhesion, biocompatibility, and injectability and could be used for target-vessel embolization in an internal iliac artery hemorrhage model. The MSAH can achieve main artery embolization without affecting the peripheral artery blood supply, resulting in both short-term rapid hemostasis and long-term degradation, with no target organ necrosis.

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Successful orthotopic uterine allotransplantation in a rabbit model using aorta and cava anastomoses: a short-term viability study

Cited by 3 publications

References 31 publications

Morphologic assessment of hypertonic citrate adenine, histidine–tryptophan–ketoglutarate, and university of Wisconsin solutions for hypothermic uterus preservation in rats

Morphologic assessment of hypertonic citrate adenine, histidine–tryptophan–ketoglutarate, and university of Wisconsin solutions for hypothermic uterus preservation in rats

Human uterine vasculature with respect to uterus transplantation: A comprehensive review

Application of modified sodium alginate hydrogel for interventional embolization of hemorrhagic diseases

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