Successful long-term preservation of abscisic-acid-treated and desiccated carrot somatic embryos

Shiota, Hajime; Tachibana, Kunihide; Watabe, Ken-ichi; Kamada, Hiroshi

doi:10.1007/s002990050654

Cited by 17 publications

(10 citation statements)

References 29 publications

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“…Many approaches, e.g., slow cooling, vitrification, encapsulation-dehydration (ED), and encapsulation-vitrification (EV) method, were developed, and used with varying degrees of success to preserve plants. The organs/tissues that were successfully cold stored or cryopreserved mainly included shoot tips (Pennycooke et al 2000;Lambardi et al 2000;Xu et al 2002), callus and somatic embryo (Ford et al 2000;Shiota et al 1999). However, only a few studies have evaluated preservation for HRs (Benson and Hamill 1991;Teoh 1996;Yoshimatsu et al 1996;Phunchindawn et al 1997;Hirata et al 2002), and results varied by plant species and methods used.…”

Section: Introductionmentioning

confidence: 98%

Cold storage and cryopreservation of hairy root cultures of medicinal plant Eruca sativa Mill., Astragalus membranaceus and Gentiana macrophylla Pall.

Xue

Luo

et al. 2007

Plant Cell Tiss Organ Cult

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To explore the possibility of an effectively long-term preservation of the germplasm of the HR lines of medicinal plant Astragalus membranaceus, Gentiana macrophylla Pall., and Eruca sativa Mill., both cold storage and cryopreservation approaches were attempted and compared. After 5-month cold storage on half strength Murashige and Skoog (1962) (1/2 MS) agar medium (AM), up to 82.9, 75.7, and 100% of the A. membranaceus, G. macrophylla and E. sativa hairy roots (HRs) recovered growth, respectively. The survival rates of A. membranaceus and G. macrophylla HRs significantly decreased, whereas that of E. sativa HR was unchanged with the addition of increased levels of exogenous abscisic acid (ABA) during cold storage. Using the encapsulation-vitrification (EV) method for cryopreservation, the G. macrophylla HRs died, whereas up to 6 and 73% of the A. membranaceus and E. sativa HRs survived, respectively. The HR lines evaluated with both methods showed no significant differences in morphology and growth rate compared with controls that were not subjected to preservation methods. These results suggest that cold storage is a more suitable alternative for the HR lines of the three studied plant species and that specificity of plant species have profound effects on the effectiveness of preservation.

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Section: Introductionmentioning

confidence: 98%

Cold storage and cryopreservation of hairy root cultures of medicinal plant Eruca sativa Mill., Astragalus membranaceus and Gentiana macrophylla Pall.

Xue

Luo

et al. 2007

Plant Cell Tiss Organ Cult

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“…Sucrose, due to its solute properties, also promotes glass phase formation in the cytoplasm (Koster, 1991). ABA plays an important role in desiccation tolerance of several plant species (Senaratna et al, 1989;Shiota et al, 1999). ABA is associated with synthesis of proteins (Seijo, 2000) and compatible solute components, which are important in plant stress tolerance (Fang et al, 2004).…”

Section: Ln:liquid Nitrogenmentioning

confidence: 99%

Effect of Abscisic Acid on Survival and Recovery of Cryopreserved Plumule Explants of Coconut (<i>Cocos nucifera</i>)

Bandupriya

Fernando

Verdeil

et al. 2009

COCOS

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ABSRACTCryopreservation has great potential for long-term storage of coconut genetic resources. The recent development of the encapsulation-dehydration cryogenic technique dramatically facilitated progress with cryopreservation. The present study tested the effect of abscisic acid in the encapsulationdehydration method for cryopreservation of coconut plumules. Plumules were excised from mature zygotic embryos of Coconut var. Sri Lanka Tall and encapsulated in calcium alginate. The alginate beads were used to test effect of different cryoprotection pretreatments (mixtures of 0.75 M sucrose and abscisic acid (ABA) at concentrations of 0, 10, 20 and 40 µM), and exposure to silica gel for 16 hours for further dehydration, prior to freezing in liquid nitrogen. Effect of the treatments without freezing was also tested. The water loss from alginate beads following sucrose incubation and dehydration was monitored, and the survival and growth of plumules assessed at different stages.Addition of ABA to the sucrose pretreatment medium increased the survival rate of unfrozen plumules from 60% to over 80%. Pretreatment with 40 µM ABA gave the highest survival rate (84%) of frozen plumules. The recovery rate of plumules (frozen and unfrozen) pretreated without ABA was very low (6-8%). The addition of ABA increased the recovery rate significantly; frozen plumules that had been treated with 40 µM ABA recorded a 39% recovery rate. The improved recovery could be ascribed to increased water loss (69%) in plumules treated with 40 µM ABA.

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“…Increased ABA stimulates the expression of a number of stress-related genes such as those of late embryogenesis abundant (LEA) proteins, which are thought to protect cells from the damage caused by the dehydration stress (Chandler and Robertson, 1994). It has been demonstrated that various tissue-cultured cells of vascular plants, including those of angiosperms, gymnosperms, and ferns, develop desiccation and freezing tolerance upon exogenous ABA treatment, indicating that ABA plays a key role in stress tolerance at cellular levels (Reynolds and Bewley, 1993;Attree et al, 1995;Shiota et al, 1999;Gusta et al, 2005).…”

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confidence: 99%

Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

et al. 2010

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Abscisic acid (ABA) is postulated to be a ubiquitous hormone that plays a central role in seed development and responses to environmental stresses of vascular plants. However, in liverworts (Marchantiophyta), which represent the oldest extant lineage of land plants, the role of ABA has been least emphasized; thus, very little information is available on the molecular mechanisms underlying ABA responses. In this study, we isolated and characterized MpABI1, an ortholog of ABSCISIC ACID INSENSITIVE1 (ABI1), from the liverwort Marchantia polymorpha. The MpABI1 cDNA encoded a 568-amino acid protein consisting of the carboxy-terminal protein phosphatase 2C (PP2C) domain and a novel amino-terminal regulatory domain. The MpABI1 transcript was detected in the gametophyte, and its expression level was increased by exogenous ABA treatment in the gemma, whose growth was strongly inhibited by ABA. Experiments using green fluorescent protein fusion constructs indicated that MpABI1 was mainly localized in the nucleus and that its nuclear localization was directed by the aminoterminal domain. Transient overexpression of MpABI1 in M. polymorpha and Physcomitrella patens cells resulted in suppression of ABA-induced expression of the wheat Em promoter fused to the b-glucuronidase gene. Transgenic P. patens expressing MpABI1 and its mutant construct, MpABI1-d2, lacking the amino-terminal domain, had reduced freezing and osmotic stress tolerance, and associated with reduced accumulation of ABA-induced late embryogenesis abundant-like boiling-soluble proteins. Furthermore, ABA-induced morphological changes leading to brood cells were not prominent in these transgenic plants. These results suggest that MpABI1 is a negative regulator of ABA signaling, providing unequivocal molecular evidence of PP2C-mediated ABA response mechanisms functioning in liverworts.Land plants are repeatedly challenged by environmental stresses such as desiccation, salinity, and freezing, which can cause irreversible damage to intracellular structures and membranes by severe dehydration. Abscisic acid (ABA), which is known to be a phytohormone responsible for physiological control of seed maturation and germination, bud dormancy, and stomata closure, has been postulated to be involved in the development of cellular dehydration tolerance under stress conditions. In higher plants, water-stress conditions trigger a transient or sustained increase in endogenous ABA content in tissues. Increased ABA stimulates the expression of a number of stress-related genes such as those of late embryogenesis abundant (LEA) proteins, which are thought to protect cells from the damage caused by the dehydration stress (Chandler and Robertson, 1994). It has been demonstrated that various tissue-cultured cells of vascular plants, including those of angiosperms, gymnosperms, and ferns, develop desiccation and freezing tolerance upon exogenous ABA treatment, indicating that ABA plays a key role in stress tolerance at cellular

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Successful long-term preservation of abscisic-acid-treated and desiccated carrot somatic embryos

Cited by 17 publications

References 29 publications

Cold storage and cryopreservation of hairy root cultures of medicinal plant Eruca sativa Mill., Astragalus membranaceus and Gentiana macrophylla Pall.

Cold storage and cryopreservation of hairy root cultures of medicinal plant Eruca sativa Mill., Astragalus membranaceus and Gentiana macrophylla Pall.

Effect of Abscisic Acid on Survival and Recovery of Cryopreserved Plumule Explants of Coconut (<i>Cocos nucifera</i>)

Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

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