Successful in vitro production of embryos in the red deer ( ) and the sika deer ( )

Comizzoli, Pierre; Mermillod, Pascal; Cognie, Y.; Chai, Norin; Legendre, X.; Mauget, R.

doi:10.1016/s0093-691x(01)00433-2

Cited by 64 publications

(57 citation statements)

References 23 publications

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“…This would facilitate the creation of germplasm banks of not only endangered species (Ptak et al 2002, Kozdrowski et al 2011) but also those that could be at risk in the future (Holt and Pickard 1999). In addition, epididymal spermatozoa collected postmortem could be used for artificial insemination (Garde et al 2006, Morrow et al 2009), in vitro fertilization (Garcia-Alvarez et al 2009) and in vitro embryo production (Comizzoli et al 2001, Martins et al 2007). …”

Section: Discussionmentioning

confidence: 99%

Morphological defects of epididymal spermatozoa in male roe deer (Capreolus capreolus) during the reproductive season

Kozioł¹,

Koziorowski²

2015

Polish Journal of Veterinary Sciences

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The aim of this study was to analyse the morphology of the epididymal spermatozoa of male roe deer obtained postmortem at the beginning (May), peak (July/August) and the end (September) of the reproductive season. Spermatozoal abnormalities were divided into major (associated with impaired fertility) and minor (not associated with impaired fertility) defects. The highest percentage of abnormal spermatozoa was observed in May (17.78 ± 1.88%), with a much higher proportion of major (12.35 ± 1.11%) than minor defects (5.43 ± 1.59%) being observed. The percentage of abnormal spermatozoa was lowest during the peak of the reproductive season (4.97 ± 1.13%), with the proportion of major (2.68 ± 0.78%) and minor defects (2.28 ± 0.45%) being comparable during this period. The percentage of abnormal spermatozoa increased again in September (11.05 ± 1.60%), with the major defects (6.15 ± 1.04%) slightly surpassing the minor defects (4.90 ± 0.77%); however, total abnormalities still remained lower than those found in May. These differences were statistically significant, with the exception of the difference in minor defects between the pre-rut and post-rut periods. These results indicate that the best period to collect epididymal spermatozoa from roe deer postmortem is the peak of the reproductive season (July/August); however, they can also be recovered at the end of the reproductive season (September), as the percentage of major defects is relatively low at this time. This study provides the basis for further research to determine optimal methods for the storage and cryopreservation of epididymal spermatozoa in this species.

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Section: Discussionmentioning

confidence: 99%

Morphological defects of epididymal spermatozoa in male roe deer (Capreolus capreolus) during the reproductive season

Kozioł¹,

Koziorowski²

2015

Polish Journal of Veterinary Sciences

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“…The culture medium was not changed during the 7 days. Uncleaved embryos were removed from culture and fixed at 32 hpi, and the nuclear status was evaluated [43,44]. Retrospective analysis of oocyte maturation or successful fertilization was determined through visualization of the nucleus or imaging of pronuclei poststaining.…”

Section: Oocyte Assessments Fertilization and Embryo Developmentmentioning

confidence: 99%

Increasing Age Influences Uterine Integrity, But Not Ovarian Function or Oocyte Quality, in the Cheetah (Acinonyx jubatus)1

Crosier

Comizzoli

Baker

et al. 2011

Biology of Reproduction

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Although the cheetah (Acinonyx jubatus) routinely lives for more than 12 yr in ex situ collections, females older than 8 yr reproduce infrequently. We tested the hypothesis that reproduction is compromised in older female cheetahs due to a combination of disrupted gonadal, oocyte, and uterine function/integrity. Specifically, we assessed 1) ovarian response to gonadotropins; 2) oocyte meiotic, fertilization, and developmental competence; and 3) uterine morphology in three age classes of cheetahs (young, 2-5 yr, n = 17; prime, 6-8 yr, n = 8; older, 9-15 yr, n = 9). Ovarian activity was stimulated with a combination of equine chorionic gonadotropin and human chorionic gonadotropin (hCG), and fecal samples were collected for 45 days before gonadotropin treatment and for 30 days after oocyte recovery by laparoscopy. Twenty-six to thirty hours post-hCG, uterine morphology was examined by ultrasound, ovarian follicular size determined by laparoscopy, and aspirated oocytes assessed for nuclear status or inseminated in vitro. Although no influence of age on fecal hormone concentrations or gross uterine morphology was found (P > 0.05), older females produced fewer (P < 0.05) total antral follicles and oocytes compared to younger counterparts. Regardless of donor age, oocytes had equivalent (P > 0.05) nuclear status and ability to reach metaphase II and fertilize in vitro. A histological assessment of voucher specimens revealed an age-related influence on uterine tissue integrity, with more than 87% and more than 56% of older females experiencing endometrial hyperplasia and severe pathologies, respectively. Our collective findings reveal that lower reproductive success in older cheetahs appears to be minimally influenced by ovarian and gamete aging and subsequent dysfunction. Rather, ovaries from older females are responsive to gonadotropins, produce normative estradiol/progestogen concentrations, and develop follicles containing oocytes with the capacity to mature and be fertilized. A more likely cause of reduced fertility may be the high prevalence of uterine endometrial hyperplasia and related pathologies. The discovery that a significant proportion of oocytes from older females have developmental capacity in vitro suggests that in vitro fertilization and embryo transfer may be useful for "rescuing" the genome of older, nonreproductive cheetahs.

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“…However, sika deer before application to related endangered subspecies [14,45]. Maturation rates were about 75-80%, and fertilization rates were 60% (with ejaculated or epididymal sperm).…”

Section: Induction Of Ovulation Superovulation Embryo Collection Anmentioning

confidence: 99%

Reproductive biotechnologies for endangered mammalian species

2000

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-Assisted reproductive techniques (gamete cryopreservation, artificial insemination, embryo transfer, and in vitro fertilization) allow to propagate small fragmented populations of wild endangered species or domestic breeds. There are the best way for producing several offspring from selected genitors in order to avoid inbreeding depression. However, few mammalian species have been well studied for their reproductive biology whereas huge differences have been observed between these species. Furthermore, materials, methods and experimental designs have to be adapted for each case and each limiting factor (wildness, poor quantity of biological material, disparate locations). Genome resource banking is currently arising and the most applied reproductive biotechnology remains artificial insemination. Assisted reproductive techniques currently developed in domestic species (intracytoplasmic sperm injection, nuclear transfer) may offer new opportunities for the propagation of endangered species. endangered species / artificial insemination / cryopreservation / in vitro fertilization / embryo Résumé -Biotechnologies de la reproduction appliquées aux mammifères en voie de disparition. Les techniques de reproduction assistée (cryoconservation des gamètes, insémination artificielle, transfert embryonnaire, fécondation in vitro) permettent d'accroître des populations, parfois dispersées géographiquement, d'espèces sauvages ou domestiques en voie de disparition. Ces méthodes sont le meilleur moyen pour produire plusieurs descendants à partir de géniteurs sélectionnés de façon à éviter la consanguinité. La biologie de la reproduction est connue pour peu d'espèces de mammifères alors que de grandes différences ont pourtant été mises en évidence entre ces espèces. En outre, les matériels, les méthodes, ainsi que les schémas expérimentaux doivent être adaptés à chaque cas et pour chaque facteur limitant (animaux sauvages, peu de matériel biologique, populations dispersées). Des banques génétiques sont actuellement en voie de constitution mais la technique la plus utilisée reste l'insémination artificielle. Cependant, des biotechnologies de la reproduction en cours de mise au point chez les animaux domestiques (injection intra-cytoplasmique de spermatozoïde, transfert nucléaire) pourraient offrir de nouvelles possibilités pour l'accroissement de populations menacées. espèces menacées / insémination artificielle / congélation / fécondation in vitro / embryon Reprod. Nutr. Dev. 40 (2000)

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Successful in vitro production of embryos in the red deer ( ) and the sika deer ( )

Cited by 64 publications

References 23 publications

Morphological defects of epididymal spermatozoa in male roe deer (Capreolus capreolus) during the reproductive season

Morphological defects of epididymal spermatozoa in male roe deer (Capreolus capreolus) during the reproductive season

Increasing Age Influences Uterine Integrity, But Not Ovarian Function or Oocyte Quality, in the Cheetah (Acinonyx jubatus)1

Reproductive biotechnologies for endangered mammalian species

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