Successful Establishment of in Vitro Cultures of Prunus Cerasifera Hybrids by Embryo Culture of Immature Fruits

Arbeloa, A.; Daorden, M. E.; García, Eva de; Marín, Juan Antonio Antolinos

doi:10.17660/actahortic.2003.616.56

Cited by 9 publications

(8 citation statements)

References 1 publication

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“…A significant percentage of embryos were smaller than 8 mm at harvest, what is considered as abortive seeds (Arbeloa et al, 2000). In optimal in vitro germination conditions, germination rate was 95.6% for embryos larger than 8 mm (non-abortive seeds) and 60% for the embryos smaller than 8 mm (abortive seeds) (Daorden at al., 2002;Arbeloa et al, 2003;Daorden et al 2004). Moreover, whereas in vitro multiplication of Myrobalan is easy and rapid (Hammerschlag, 1982) apricot is considered a recalcitrant species (Marino et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

“…Germination and survival rates were recorded for each treatment. Shoots were later rooted in vitro in half MS medium with 5M IBA (Arbeloa et al, 2003). Micropropagated plants were acclimatized (Marín, 2003) and finally transplanted to orchard conditions.…”

Section: Embryo Rescue and In Vitro Culturementioning

confidence: 99%

“…Embryo abortion and fruit abscission are frequently the result of postzygotic incompatibility barriers (Stebbins, 1958) that make seeds unviable. However, the development of embryo rescue techniques applied after fertilization has facilitated hybrid obtention (Ramming, 1990), allowing embryo development and in vitro germination of unviable seeds, becoming a useful tool in Prunus breeding programs (Arbeloa et al, 2000(Arbeloa et al, , 2003.…”

Section: Introductionmentioning

confidence: 99%

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Significant effect of accidental pollinations on the progeny of low setting Prunus interspecific crosses

et al. 2006

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SummaryThe incidence of fortuitous pollination on interspecific hybridizations of the plum rootstock Myrobalan with the apricot cultivars 'Moniquí' and 'Moniquí Borde' was assessed in this work. Progeny was originated through hand pollination of emasculated flowers of three Myrobalan clones, without bagging, in 1998 and 1999. Fruit set was low and variable among years (1.8-8.0%), but higher than the level of accidental pollination measured with emasculated and non-pollinated flowers (1.2%). Molecular characterization of the progeny was performed with three SSR markers showing that only 28% of the seedlings, obtained by in vitro germination and culture of immature embryos, were hybrids. This represents a lower percentage than expected, and is explained here by the low viability of hybrid embryos and seedlings. The use of molecular markers is discussed in terms of a convenient method for an early identification of putative hybrids in breeding programs with low setting crosses, where the proportion of non-hybrids is magnified.

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Section: Discussionmentioning

confidence: 99%

Section: Embryo Rescue and In Vitro Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Significant effect of accidental pollinations on the progeny of low setting Prunus interspecific crosses

et al. 2006

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“…Overcoming post-zygotic barriers and saving at least some hybrid embryos is possible through the use of the embryo-rescue technique (Golis et al 2002;Kukharchyk, Kastrickaya 2006;Liu et al 2007). It enables the development and growth of immature embryos in vitro, which makes it a very useful tool in the distant hybridization of plants of the genus Prunus (Arbeola et al 2003).…”

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confidence: 99%

Studies on the interspecific crossing compatibility among three Prunus species and their hybrids

Szymajda¹,

Napiórkowska²,

Korbin³

et al. 2015

Hort. Sci. (Prague)

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Szymajda M., Napiórkowska B., Korbin M., Żurawicz E. (2015): Studies on the interspecific crossing compatibility among three Prunus species and their hybrids. Hort. Sci. (Prague), 42: 70-82.In 2011-2013, a study on the crossing compatibility and the setting of fruit in distant hybridization within the genus Prunus, among the species P. armeniaca L. (apricot), P. salicina Lindl. (Japanese plum), P. cerasifera Ehrh. (myrobalan plum) and the hybrids P. salicina × P. cerasifera was conducted at the Institute of Horticulture in Skierniewice, Poland. The percentage of fruit set depended on the direction of pollination of the crossed species. Most fruits were obtained by crossing P. salicina × P. armeniaca and P. salicina × P. cerasifera. The largest number of fruitlets in relation to the number of pollinated flowers was obtained when two genotypes of P. salicina -Czernuszka and D 17-73, were the maternal parents. The results show that the crossing compatibility and effectiveness in the cross-breeding of the studied species of Prunus are influenced to a greater extent by the genotype of the maternal form of P. salicina than by the genotype of the paternal form of P. armeniaca and P. cerasifera.

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“…However, the plant regeneration rate was still not high enough and should be increased with more measures. In several species of Prunus, immature embryos were usually kept at a low temperature (0-4°C) more than 2 months to avoid the development of rosette type plants (Arbeloa et al, 2009;Burgos and Ledbetter, 1993;Kukharchyk and Kastrickaya, 2006). We noticed the formation of rosette-type structure without shoot tips from embryos which accounted for about 30% of the total cultured embryo in 2012.…”

Section: Discussionmentioning

confidence: 97%

Hybridization Using a New Male-sterile Germplasm as the Female Parent in Chinese Jujube

Wang¹,

Cui²,

Dai³

et al. 2015

HST

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This study identified a new male-sterile germplasm of Chinese jujube, named male-sterile No. 2 (JMS2), and achieved controlled hybridization using that germplasm as the female parent. The anthers of JMS2 before flower bud opening became shrunken, dingy yellow and much smaller than normal ones, and they changed to brown after anthesis. No pollen was observed in anthers of JMS2 and its male-sterile trait remained stable over different years. A total of 1,642 fruits were obtained from ten intra-and interspecific cross combinations via controlled hybridization from 2008 to 2012 using JMS2 as the female parent. Of those, 27.3% produced seeds, on average (0-72.6%). The rate of fruit with seed (RFS) was significantly different between cross combinations or years. Compared to other cross combinations, the RFS in the combination of JMS2 × 'Xingguang' (a Chinese jujube cultivar with high resistance to jujube witches' broom disease) and JMS2 × 'Xing16' (a sour jujube genotype) remained high in different years and reached means of 48.7 and 58.1%, respectively. Finally, 150 plantlets were regenerated from immature embryos, and 51 of them were randomly selected and identified to be authentic hybrids using amplified fragment length polymorphism (AFLP) markers. This is the first report of hybrids obtained from a cross between Chinese jujube and sour jujube.

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Successful Establishment of in Vitro Cultures of Prunus Cerasifera Hybrids by Embryo Culture of Immature Fruits

Cited by 9 publications

References 1 publication

Significant effect of accidental pollinations on the progeny of low setting Prunus interspecific crosses

Significant effect of accidental pollinations on the progeny of low setting Prunus interspecific crosses

Studies on the interspecific crossing compatibility among three Prunus species and their hybrids

Hybridization Using a New Male-sterile Germplasm as the Female Parent in Chinese Jujube

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