Successful Engraftment of Autologous and Allogeneic Islets into the Porcine Thymus

Pc, Watt; Mullen, Yoko; Nomura, Yuji; Watanabe, Yasumasa; Fc, Brunicardi; Anderson, Corey Devin; Mj, Zinner; Ep, Passaro

doi:10.1006/jsre.1994.1057

Cited by 28 publications

(8 citation statements)

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“…Previous studies by other groups have demonstrated that islet-cell transplants are protected from rejection when injected into native thymic tissue, suggesting that the thymus constitutes an immunologically privileged site (22). The present study extends those findings to demonstrate that thymic tissue may furthermore permit the successful engraftment of islet cells at sites distal to the thymus.…”

Section: Engrafted Islets In Tiks Maintained Normoglycemia Following supporting

confidence: 71%

Vascularized islet-cell transplantation in miniature swine. I. Preparation of vascularized islet kidneys

et al. 2002

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The implantation of islets beneath the autologous renal capsule permitted the establishment of a vascular supply and thereby supported normal islet-cell growth and function. The presence of thymic tissue beneath the autologous renal capsule facilitated the engraftment of minor-mismatched islets, and such grafts achieved results similar to autologous islet transplants. Therefore, the ability to create vascularized islet grafts may provide a strategy for successful islet transplantation across allogeneic and potentially across xenogeneic barriers.

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Section: Engrafted Islets In Tiks Maintained Normoglycemia Following supporting

confidence: 71%

Vascularized islet-cell transplantation in miniature swine. I. Preparation of vascularized islet kidneys

et al. 2002

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“…Indeed, an autoimmune model of type 1 diabetes in rats showed long-term survival of intrathymic transplanted islets [104]. Despite this promising immunological theory, the thymic site requires a large number of islets to reverse hyperglycemia [105–107]. …”

Section: Hepatic and Alternative Transplant Sitesmentioning

confidence: 99%

Revascularization of Transplanted Pancreatic Islets and Role of the Transplantation Site

Pepper

Gala-López

Ziff

et al. 2013

Clinical and Developmental Immunology

136

101

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Since the initial reporting of the successful reversal of hyperglycemia through the transplantation of pancreatic islets, significant research efforts have been conducted in elucidating the process of revascularization and the influence of engraftment site on graft function and survival. During the isolation process the intrinsic islet vascular networks are destroyed, leading to impaired revascularization after transplant. As a result, in some cases a significant quantity of the beta cell mass transplanted dies acutely following the infusion into the portal vein, the most clinically used site of engraftment. Subsequently, despite the majority of patients achieving insulin independence after transplant, a proportion of them recommence small, supplemental exogenous insulin over time. Herein, this review considers the process of islet revascularization after transplant, its limiting factors, and potential strategies to improve this critical step. Furthermore, we provide a characterization of alternative transplant sites, analyzing the historical evolution and their role towards advancing transplant outcomes in both the experimental and clinical settings.

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“…Our results indicate that BMSCs can transdifferentiate into IPCs in vitro without fusion, and can function both in vivo and in vitro. Many sites have been evaluated for engraftment of pancreatic islet transplantation; however, most of the experimental sites have no clinical applicability (Lee et al, 1992;Watt et al, 1994;Ferguson and Scothorne, 1977;Rayat et al, 1997;Inoue et al, 2003). The liver has been considered as the most adequate site.…”

Section: Discussionmentioning

confidence: 99%

Insulin‐Producing Cells Derived from Rat Bone Marrow and Their Autologous Transplantation in the Duodenal Wall for Treating Diabetes

Zhang

Wang

Liu

et al. 2009

The Anatomical Record

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Islet cell transplantation is one of the most promising therapies for diabetes mellitus (DM). However, the limited availability of purified islets for transplantation and the risk of immunological rejection severely limit its use. In vitro transdifferentiation of autologous bone marrow-derived mesenchymal stem cells (BMSCs) into insulin-producing cells (IPCs) could provide an abundant source of cells for this procedure and avoid immunological rejection. Here, we isolated and characterized BMSCs and induced their in vitro differentiation into IPCs. Reverse-transcription polymerase chain reaction analysis revealed that these IPCs could express Ins1, Ins2, glucagon, glucose transporter 2, and pancreatic duodenal homeobox-1. Insulin production by the IPCs was confirmed by immunocytochemistry and Western blot analysis. On this basis, donor rats supplying BMSCs were made diabetic by a single intraperitoneal injection of streptozotocin. The IPCs were then autologously transplanted into the duodenal submucosa of diabetic rats. Grafted cells could be visualized in sections after 2, 4, and 8 weeks by immunohistochemical staining for insulin. Furthermore, in the IPC-implanted group, hyperglycemia was normalized, compared with a persistent increase in glucose levels in the diabetic group and intraperitoneal glucose tolerance test-induced responses were observed in the IPC-implanted group. These results on autologous transplantation of IPCs derived from BMSCs into the duodenal wall could offer a novel potential therapeutical protocol for DM. Anat Rec, 292:728-735, 2009. V V C 2009 Wiley-Liss, Inc.

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Successful Engraftment of Autologous and Allogeneic Islets into the Porcine Thymus

Cited by 28 publications

References 0 publications

Vascularized islet-cell transplantation in miniature swine. I. Preparation of vascularized islet kidneys

Vascularized islet-cell transplantation in miniature swine. I. Preparation of vascularized islet kidneys

Revascularization of Transplanted Pancreatic Islets and Role of the Transplantation Site

Insulin‐Producing Cells Derived from Rat Bone Marrow and Their Autologous Transplantation in the Duodenal Wall for Treating Diabetes

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