Successful culmination of pregnancy and live birth following the transfer of frozen-thawed buffalo embryos

Kasiraj, R.; Misra, A. K.; Rao, M. M.; Jaiswal, R.S.; Rangareddi, N.S.

doi:10.1016/0093-691x(93)90016-x

Cited by 17 publications

(6 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Oocyte Embryo Cow 1992 [3] 1973 [4] Rabbit 1989 [5,6] 1974 [7] Sheep -1976 [8] Goat -1976 [9] Horse 2002 [10] 1982 [11] Pig 2014 [12] 1989 [13] Buffalo -1993 [14] can be avoided by exposing cells to cryoprotectants [15]. Permeating cryoprotective agent (CPA) decreases ice formation by replacing the intracellular liquid [16].…”

Section: Speciesmentioning

confidence: 99%

Cryopreservation of Oocytes and Embryos: Current Status and Opportunities

Dhali¹,

Kolte²,

Mishra³

et al. 2019

Infertility, Assisted Reproductive Technologies and Hormone Assays

View full text Add to dashboard Cite

The biochemical and metabolic activities of living cells are virtually stopped at ultralow temperature and they enter into a suspended state of animation. However, as such, exposure of living cells to ultralow temperature is associated with complex changes that reduce their survivability following freeze-thawing. Cryopreservation is the method for preserving living cells at ultralow temperature at genetically and physiologically stabilized state. Cryopreservation of oocytes and embryos is an integral part of the assisted reproductive technologies with many potential applications. Cryobanking of oocytes and embryos derived from genetically superior animals is promising for enhancing the outcome of planned breeding programs and conserving biodiversity of endangered animal species. Cryobanking can also ensure steady supply of oocytes and embryos for many downstream applications of assisted reproduction such as in vitro embryo production, embryo transfer, production of stem cells, and genetic engineering. Tremendous advancements have been made in this field over the past 5 decades and several methods have been demonstrated for cryopreserving oocytes and embryos in different species. This chapter focuses on the fundamental aspects of oocyte and embryo cryopreservation.

show abstract

Section: Speciesmentioning

confidence: 99%

Cryopreservation of Oocytes and Embryos: Current Status and Opportunities

Dhali¹,

Kolte²,

Mishra³

et al. 2019

Infertility, Assisted Reproductive Technologies and Hormone Assays

View full text Add to dashboard Cite

show abstract

“…The multiple ovulation and embryo transfer (MOET) in buffaloes resulted in very low recovery of embryos from the nonsurgical collection. The average number of embryos collected from a donor was 1.0 (Drost et al, 1988 ; Vlakhov et al, 1986 ), 2.0 (Cruz et al, 1991 ) 2.7 (Misra, 1993 ), 4.5 (Kasiraj et al, 1993 ), 1.6 (Kandil et al, 2012 ), 2.7 (Qin et al, 2012 ), and 5.8 (Singh et al, 2015 ). These outputs cover the trials made in Bulgaria, India, Philippines, Egypt, and China (Hufana‐Duran & Duran, 2015 ).…”

Section: In Vitro Embryo Productionmentioning

confidence: 99%

Current status of assisted reproductive technologies in buffaloes

Srirattana

Hufana-Durán

Atabay

et al. 2022

Animal Science Journal

View full text Add to dashboard Cite

Buffaloes are raised by small farm holders primarily as source of draft power owing to its resistance to hot climate, disease, and stress conditions. Over the years, transformation of these animals from draft to dairy was deliberately carried out through genetic improvement program leading to the development of buffalo-based enterprises. Buffalo production is now getting more attention and interest from buffalo raisers due to its socioeconomic impact as well as its contribution to propelling the livestock industry in many developing countries. Reproduction of buffaloes, however, is confronted with huge challenge and concern as being generally less efficient to reproduce compared with cattle due to both intrinsic and extrinsic factors such as poor estrus manifestation, silent heat, marked seasonal infertility, postpartum anestrus, long calving interval, delayed puberty, inherently low number of primordial follicles in their ovaries, high incidence of atresia, and apoptosis. Assisted reproductive technologies (ARTs) are major interventions for the efficient utilization of follicle reserve in buffaloes. The present review focuses on estrus and ovulation synchronization for fixed time artificial insemination, in vitro embryo production, intracytoplasmic sperm injection, cryopreservation of oocytes and embryos, somatic cell nuclear transfer, the factors affecting utilization in various ARTs, and future perspectives in buffaloes.

show abstract

“…Majority of the mammalian embryos are still frozen by conventional equilibrium methods involving controlled cooling rates which require the use of freezing machines (Palasz and Mapletoft, 1996). Using such procedures, cryopreservation of buffalo embryos has been reported (Misra et al, 1992;Kasiraj et al, 1993) but pregnancy rates are very low. Transfer of 4 embryos frozen in 1.0 M glycerol to 3 recipients resulted in 1 pregnancy vs. 2 pregnancies for 5 embryo frozen in 1.4 M glycerol and transferred to 5 recipients (Misra et al, 1992).…”

Section: Embryo Cryopreservationmentioning

confidence: 99%

Reproductive Biotechnologies for Improvement of Buffalo: The Current Status

Purohit¹,

Duggal²,

Dadarwal³

et al. 2003

Asian Australas. J. Anim. Sci

View full text Add to dashboard Cite

Reproductive biotechnologies continue to be developed for genetic improvement of both river and swamp buffalo.Although artificial insemination using frozen semen emerged some decades back, there are still considerable limitations. The major problem appears to be the lack of efficient methods for estrus detection and timely insemination. Controlled breeding experiments in the buffalo had been limited and similar to those applied in cattle. Studies on multiple ovulation and embryo transfer are essentially a replica of those in cattle, however with inherent problems such as lower number of primordial follicles on the buffalo ovary, poor fertility and seasonality of reproduction, lower population of antral follicles at all stages of the estrous cycle, poor endocrine status and a high incidence of deep atresia in ovarian follicles, the response in terms of transferable embryo recovery has remained low with 0.51 to 3.0 per donor and pregnancy rates between 15 to 30%. In vitro production of buffalo embryos is a valid alternative to recovery of embryos by superovulation. This aspect received considerable attention during the past decade, however the proportion of embryos that develops to the blastocyst stage is still around 25-30% and hence the in vitro culture procedures need substantial improvement. Embryo cryopreservation procedures for direct transfer post thaw need to be developed for bubaline embryos. Nuclear transfer and embryo cloning is a technique that has received attention in various species during recent years and can be of immense value in buffaloes as they have a low rate of embryo recoveries by both in vitro and in vivo procedures. Gender pre-selection, genome analysis, gene mapping and gene transfer are a few of the techniques that have been studied to a limited extent during recent years and are likely to be included in future studies on buffaloes. Very recently, reproductive biotechnologies have been applied to feral buffaloes as well, but the results obtained so far are modest. When fully exploited they can play an important role in the preservation of endangered species.

show abstract

Successful culmination of pregnancy and live birth following the transfer of frozen-thawed buffalo embryos

Cited by 17 publications

References 6 publications

Cryopreservation of Oocytes and Embryos: Current Status and Opportunities

Cryopreservation of Oocytes and Embryos: Current Status and Opportunities

Current status of assisted reproductive technologies in buffaloes

Reproductive Biotechnologies for Improvement of Buffalo: The Current Status

Contact Info

Product

Resources

About