Successful control of the first OXA-48 and/or NDM carbapenemase-producing Klebsiella pneumoniae outbreak in Slovenia 2014–2016

Pirš, Mateja; Kišek, Tjaša Cerar; Hergouth, Veronika Križan; Seme, Katja; Premru, Manica Mueller; Jeverica, Samo; Logar, Mateja; Mrvič, Tatjana; Žnidaršič, B.; Markočič, O. Jordan; Zupanc, Tatjana Lejko

doi:10.1016/j.jhin.2018.10.022

Cited by 18 publications

(19 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Almost identical plasmid was confirmed in different outbreak isolates belonging to the two STs suggesting plasmid-mediated spread of blaOXA-48 resistance genes. This is in concordance with initial molecular analysis of the outbreak where PFGE demonstrated oligoclonal structure in epidemiologically clearly linked patients [15]. A recent study shows that the most common and high-risk clonal lineages in Europe are ST11, ST15, ST101 and ST258 and their derivatives [4], of which STs 15, 101 and 258 were also detected among our isolates.…”

Section: Plos Onesupporting

confidence: 92%

“…A total of 32 K. pneumoniae isolates were included in this study. Outbreak isolates were selected based on PFGE profiles [15]. The remaining isolates were selected to reflect the Slovenian population of carbapenemases.…”

Section: Selection Of Isolatesmentioning

confidence: 99%

“…Multiplex real-time PCR targeting the genes blaNDM, blaKPC, blaOXA-48-like, blaIMP and blaVIM was performed using the LightMix Modular Carbapenemase kits (TIB Molbiol, Berlin, Germany) [15].…”

Section: Molecular Detection Of Blandm Blakpc Blaoxa-48-like Blaimmentioning

confidence: 99%

“…hospital outbreak with aforementioned ST437 and ST147, that occurred in 2014-2016 [15], with OXA-48-and/or NDM-1-producing K. pneumoniae. Almost identical plasmid was confirmed in different outbreak isolates belonging to the two STs suggesting plasmid-mediated spread of blaOXA-48 resistance genes.…”

Section: Plos Onementioning

confidence: 99%

“…Two patients had also other CPE in combination with CP-K. pneumoniae. The outbreak was investigated with classic epidemiological investigation and genotyping of the isolates was performed using pulse-field gel electrophoresis and MLST determination [15]. While data obtained using classical approach can provide a general overview of the situation, data obtained using whole genome sequencing (WGS) gives much more detailed and relatively rapid insight into the situation as WGS not only has much higher discriminatory power than PFGE [16], but also allows simultaneous detection of antimicrobial resistance genes, plasmids and virulence factors.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Whole genome sequencing characterization of Slovenian carbapenem-resistant Klebsiella pneumoniae, including OXA-48 and NDM-1 producing outbreak isolates

et al. 2020

View full text Add to dashboard Cite

The first hospital outbreak of carbapenemase-producing Enterobacteriaceae in Slovenia occurred in 2014-2016. Whole genome sequencing was used to analyse the population of carbapenem-resistant Klebsiella pneumoniae collected in Slovenia in 2014-2017, including OXA-48 and/or NDM-1 producing strains from the outbreak. Methods A total of 32 K. pneumoniae isolates were analysed using short-read sequencing. Multilocus sequence typing and core genome multi-locus sequence typing were used to infer genetic relatedness. Antimicrobial resistance markers, virulence factors, plasmid content and wzi types were determined. Long-read sequencing was used for six isolates for detailed analysis of plasmids and their possible transmission. Results Overall, we detected 10 different sequence types (STs), the most common being ST437 (40.6%). Isolates from the initial outbreak belonged to ST437 (12/16) and ST147 (4/16). A second outbreak of four ST15 isolates was discovered. A new ST (ST3390) and two new wzi types (wzi-556, wzi-559) were identified. blaOXA-48 was found in 17 (53.1%) isolates, blaNDM-1 in five (15.6%), and a combination of blaOXA-48/NDM-1 in seven (21.9%) isolates. Identical plasmids carrying blaOXA-48 were found in outbreak isolates sequenced with long-read sequencing technology.

show abstract

Section: Plos Onesupporting

confidence: 92%

Section: Selection Of Isolatesmentioning

confidence: 99%

Section: Molecular Detection Of Blandm Blakpc Blaoxa-48-like Blaimmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations