Successful chimera production in the Hungarian goose (Anser anser domestica) by intracardiac injection of blastodermal cells in 3-day-old embryos

Abstract

The conservation of genetic resources of avian species has become increasingly important over the past decade. The aim of the present study was to develop a genome preservation technique for the Hungarian goose Anser anser domestica. To this end, we developed a novel approach combining the simplicity of isolating a blastodermal cell suspension, which includes forming primordial germ cells (PGCs), with the efficiency of targeting future gonads by injecting these cells into the cardiac vein of the developing host embryo. First, we determined that the migratory period of PGCs in goose embryos was between 69 and 84h of development. Then, we injected the blastodermal cell suspension into the bloodstream of recipient embryos at this stage of development and monitored donor cell transmission into the genital tract. In all, 249 embryos were injected; three were found to be chimeras in gonadal tissues, whereas only one was a chimera in other tissues. Based on these results, it is concluded that this method is suitable for producing chimeras in the domestic goose. The optimal time of cell injection was found to be between 74 and 76h. The present study is the first report of the generation of chimeras in the domestic goose using intracardiac transplantation of embryonic cells.

Finding an Effective Freezing Protocol for Turkey Semen: Benefits of Ficoll as Non-Permeant Cryoprotectant and 1:4 as Dilution Rate

“…Semen cryopreservation is the most suitable technology for the ex situ in vitro conservation of avian genetic resources and preservation of rare breeds since it is the only non-invasive and the least expensive in vitro method available to date [3,[9][10][11]. However, other methods within ex situ in vitro strategies including primordial germ cells and gonadic tissues technologies are still in progress but may be seen as methods that are complementary to semen cryopreservation [3,[12][13][14]. Hence, the obtainment of an effective semen cryopreservation protocol in avian species represents a key point for ensuring the long-term conservation of genetic diversity through the creation of a semen cryobank.…”

Sucrose increases the quality and fertilizing ability of cryopreserved chicken sperms in contrast to raffinose

“…The more feasible and available method for management of reproductive cells in bird populations is, therefore, semen cryopreservation (Blesbois et al., 2007). Other methods including primordial germ cells and gonadic tissues technologies are still in progress but may be seen as methods that are complementary to semen cryopreservation (Liptoi et al., 2013; Nakamura, 2016; Sztan et al., 2017; Thélie et al., 2018). Different freezing procedures have been developed to cryopreserve avian semen (Blesbois et al., 2007; Santiago-Moreno et al., 2012; Rakha et al., 2016, 2017a; 2018a,b,c; Thananurak et al., 2016; Chuaychu-noo et al., 2017; Thananurak et al., 2017).…”

Investigation of the Guinea fowl and domestic fowl hybrids as potential surrogate hosts for avian cryopreservation programmes

“…According to the International Union for Conservation of Nature and Natural Resources 14% of avian species are listed as threatened with extinction 1 . Therefore, it is extremely important to develop integrated systems for avian germplasm conservation; however, techniques established for genetic conservation for mammals cannot be transferred directly to avian species, mainly because of the unique physiological and anatomical characteristics of the egg 2 .…”