Successful capture of Toxocara canis larva antigens from human serum samples

Rodríguez-Caballero, Aarón; Martínez-Gordillo, Mario Noé; Medina-Flores, Yolanda; Medina-Escutia, María Edith; Meza-Lucas, Antonio; Correa, Dolores; Caballero-Salazar, Silvia; Ponce-Macotela, Martha

doi:10.1186/s13071-015-0875-5

Cited by 16 publications

(15 citation statements)

References 20 publications

(19 reference statements)

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“…Recent attempts comparing recombinant ES antigens from both Toxocara species also showed considerable cross-reactivity [ 64 , 65 ] and initial experiments using monoclonal antibodies raised against T . canis antigens in a capture ELISA to detect circulating larval antigens showed high specificity (100%) but only low sensitivity (31%) to detect human toxocarosis [ 66 ]. The overall seroprevalence of 14.2% observed in Berlin is quite high in comparison to what has been described in other studies in central Europe.…”

Section: Discussionmentioning

confidence: 99%

Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany

et al. 2017

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Rodents are important intermediate and paratenic hosts for carnivore parasites, including the important zoonotic agents Toxoplasma, Echinococcus and Toxocara. Monitoring of such parasites in rodents can be used to detect increasing risks for human and veterinary public health. Rodents were trapped at four sites in Berlin, two near the city center, two at the periphery. PCRs were conducted to detect Coccidia (target ITS-1) and specifically Toxoplasma gondii (repetitive element) in brain and ascarids (ITS-2) in muscle or brain tissue. During necropsies, metacestodes were collected and identified using ITS-2 and 12S rRNA PCRs. An ELISA to detect antibodies against Toxocara canis ES antigens was performed. Within the 257 examined rodents, the most frequently observed parasite was Frenkelia glareoli predominantly found in Myodes glareolus. T. gondii was only detected in 12 rodents and Microtus spp. (although strongly underrepresented) had a significantly increased chance of being positive. Neither Echinococcus nor typical Taenia parasites of dogs and cats were found but Mesocestoides litteratus and Taenia martis metacestodes were identified which can cause severe peritoneal or ocular cysticercosis in dogs, primates and humans. Using PCR, the ascarids T. canis (n = 8), Toxocara cati (4) and Parascaris sp. (1) were detected predominantly in muscles. Seroprevalence of T. canis was 14.2% and ELISA was thus more sensitive than PCR to detect infection with this parasite. Non-parametric multidimensional scaling and cluster analysis revealed that parasite communities could be grouped into an urban and a peri-urban cluster with high frequency of ascarid-positive rodents in urban and high frequency of F. glareoli in peri-urban sites. Prevalence rates of parasites in rodents with potential impact for human or veterinary public health are considerable and the monitoring of transmission cycles of carnivore parasites in intermediate rodent hosts is recommended to estimate the health risks arising from wild and domesticated carnivores.

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Section: Discussionmentioning

confidence: 99%

Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany

et al. 2017

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“…Serum samples were subjected to immune complex dissociation (ICD) to dissociate possible endogenous antibodies that can potentially block the Nb binding sites of TES antigens [18]. Briefly, 250 µl of serum was mixed with 250 µl of 100% glycerol and thoroughly vortexed.…”

Section: Serum Immunocomplex Dissociation (Icd)mentioning

confidence: 99%

“…Other immunoassays showed a LOD of 78 ng/ml [17], 4 ng/ml [27] and 5 ng/ml [16]. The most recent attempt to detect TES antigen in clinical samples reported a LOD of 0.440 ng/ml [18]. These assays estimated the LOD by a standard curve constructed in PBS-Tween 20 as diluent.…”

Section: Tes Quantificationmentioning

confidence: 99%

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Electrochemical detection of Toxocara canis excretory-secretory antigens in children from rural communities in Esmeraldas Province, Ecuador: association between active infection and high eosinophilia

et al. 2020

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Background: The diagnosis of active Toxocara canis infections in humans is challenging. Larval stages of T. canis do not replicate in human tissues and disease may result from infection with a single T. canis larva. Recently, we developed a nanobody-based electrochemical magnetosensor assay with superior sensitivity to detect T. canis excretorysecretory (TES) antigens. Here, we evaluate the performance of the assay in children from an Ecuadorian birth cohort that followed children to five years of age. Methods: Samples were selected based on the presence of peripheral blood eosinophilia and relative eosinophil counts. The samples were analyzed by the nanobody-based electrochemical magnetosensor assay, which utilizes a bivalent biotinylated nanobody as capturing agent on the surface of streptavidin pre-coated paramagnetic beads. Detection was performed by a different nanobody chemically labelled with horseradish peroxidase. Results: Of 87 samples tested, 33 (38%) scored positive for TES antigen recognition by the electrochemical magnetosensor assay. The average concentration of TES antigen in serum was 2.1 ng/ml (SD = 1.1). The positive result in the electrochemical assay was associated with eosinophilia > 19% (P = 0.001). Parasitological data were available for 57 samples. There was no significant association between positivity by the electrochemical assay and the presence of other soil-transmitted helminth infections. Conclusions: Our nanobody-based electrochemical assay provides highly sensitive quantification of TES antigens in serum and has potential as a valuable tool for the diagnosis of active human toxocariasis.

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“…Secondary antibody anti‐mouse IgG conjugated with horseradish peroxidase (1:500) was added, and the plate was incubated for 2 h at 37°C. The chromogen was added, the reaction was stopped with 2N sulfuric acid, and the plates were read at 490 nm in a spectrophotometer (Multiskan Go; Thermo Fisher Scientific) 26,27 …”

Section: Methodsmentioning

confidence: 99%

Detection of antigens and anti‐Toxocara canis antibodies in children with different asthma severities

Guadalupe

Noé

Esteban

et al. 2021

Immunity Inflam & Disease

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Introduction Toxocara canis can produce or exacerbate asthma, and the detection of anti‐T. canis immunoglobulin G (IgG) does not discriminate between recent infection or active larva migrans. In this study, we searched for T. canis third‐stage larval antigens (L3TES) and anti‐T. canis antibodies in children with different severities of asthma, controlled or uncontrolled. Methods A total of 145 patients with asthma who were previously diagnosed using the Global Initiative for Asthma guidelines were included. The asthma control was evaluated with the Asthma Control Questionnaire (ACQ). Enzyme‐linked immunosorbent assay was performed for the detection of L3TES; IgG was detected using sera preadsorbed with Ascaris antigens (native kit), and a commercial kit (IgG) was used as the gold standard. Results L3TES was found in 2 patients (1.37%). One had L3TES and anti‐T. canis IgG, suggesting active larva migrans. In the other patient, only L3TES was detected, likely because an infection had begun. The seroprevalence with the commercial kit and native kit was 6.2% and 17.93%, respectively. There was no significant association among asthma severity, ACQ and T. canis seroprevalence (p > .05). Conclusion It is possible to detect L3TES in patients with asthma. Two complementary techniques that can determine the infection status with T. canis and rule out cross‐reactions involve the detection of L3TES and IgG using sera preadsorbed with Ascaris antigen. There was no significant association among asthma severity, ACQ and T. canis seroprevalence.

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Successful capture of Toxocara canis larva antigens from human serum samples

Cited by 16 publications

References 20 publications

Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany

Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany

Electrochemical detection of Toxocara canis excretory-secretory antigens in children from rural communities in Esmeraldas Province, Ecuador: association between active infection and high eosinophilia

Detection of antigens and anti‐Toxocara canis antibodies in children with different asthma severities

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