Successful abrogation by thymoquinone against induction of diabetes mellitus with streptozotocin via nitric oxide inhibitory mechanism

El-Mahmoudy, Abubakr; Shimizu, Yasutake; Shiina, Takahiko; Matsuyama, Hayato; Elsayed, Mohamed L.; Takewaki, Tadashi

doi:10.1016/j.intimp.2004.09.001

Cited by 65 publications

(36 citation statements)

References 39 publications

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“…TQ was found to have no effect on either IkB degradation or NFkB activation; although it significantly inhibited both p44/42 and p38 mitogen-activated protein kinases (MAPKs) which contribute to the transcriptional machinery of inducible nitric oxide synthase and NO production, respectively. These data emphasize the protective role of TQ against development of type I diabetes via NO inhibitory pathway [56]. TQ also normalises the elevated nitrites in vitro [57].…”

Section: Possible Mechanisms Of Antidiabetic Activitysupporting

confidence: 54%

Antidiabetic Properties of a Spice Plant Nigella sativa

Mathur

2011

J Endocrinol Metab

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Seeds of Nigella sativa (black cumin/kalonji) used in pickles as spice, have also been traditionally used in treatment of many diseases including diabetes and hypertension. Among many activities exhibited by N. sativa and its constituents in animal experiments, antidiabetic property is most important. Thymoquinone (TQ), a volatile oil, is one of its active constituents but antidiabetic activity has also been shown by its aqueous extract and defatted extract. N. sativa may be beneficial in diabetic individuals and those with glucose intolerance as it reduces appetite, glucose absorption in intestine, hepatic gluconeogenesis, blood glucose level, cholesterol, triglycerides, body weight and simulates glucose induced secretion of insulin from beta-cells in pancreas; improves glucose tolerance as efficiently as metformin; yet it has not shown significant adverse effects and has very low toxicity. In streptozotocin (STZ) induced diabetic rats it causes gradual partial regeneration of pancreatic beta-cells, increases the lowered serum insulin concentrations and decreases the elevated serum glucose. N. sativa has antioxidant activity and protective role of TQ against development of type I diabetes may be via NO inhibitory pathway. It also exerts an insulin-sensitizing action in hepatocytes. Seeds of N. sativa have been safely consumed by human patients in many clinical trials which however were not aimed to assess its antidiabetic activity. In future clinical studies may show potential of N. sativa, its constituents or their synthetic analogues, in prevention and control of diabetes.

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Section: Possible Mechanisms Of Antidiabetic Activitysupporting

confidence: 54%

Antidiabetic Properties of a Spice Plant Nigella sativa

Mathur

2011

J Endocrinol Metab

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“…It was demonstrated that the only stable products formed by spontaneous decomposition of NO in oxygenated solutions are nitrites and nitrates, thus they were measured as indicators for NO production (El-Mahmoudy et al, 2005). Liver tissues were homogenized in Tris-HCl buffer (5 mM containing 2 mM EDTA, pH 7.4).…”

Section: Liver Nitritementioning

confidence: 99%

The effect of metformin and exercise on serum lipids, nitric oxide synthase and liver nitric oxide levels in streptozotocin-nicotinamide induced diabetic rats

Akyüz¹,

Tekin²,

Aydn³

et al. 2012

Afr. J. Pharm. Pharmacol.

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The objective of this study was to determine the effects of metformin treatment and exercise intervention on lipid metabolism, nitric oxide (NO), nitric oxide synthase (NOS) and glucose levels in streptozotocin-nicotinamide (STZ-NA) induced diabetic rats. Male wistar rats were used to conduct the study and they were divided into five groups of 8 rats in each. These groups are: Control group, diabetes group (STZ-NA), diabetes and metformin (DMet) group, diabetes and exercise (DE) group, and diabetes + exercise + metformin (DEMet) group. The STZ-NA induced diabetic rats were used as a model of type 2 diabetes. Metformin was orally administered to rats. For exercise intervention, rats were forced to run in a running wheel for 10 min each day (20 m/min/day). At the end of experimental period, blood glucose, glycated hemoglobin (HbA1c) levels, plasma lecithin:cholesterol acyltransferase (LCAT) levels, serum triglyceride (TG), cholesterol, high density lipoprotein (HDL), Apo A-I, NOS and liver NO levels were determined. Metformin caused beneficial changes in blood glucose, HbA1c levels, and HDL in type 2 diabetic rats. Both administration of metformin and exercise intervention independently caused beneficial changes in blood glucose. However, the surprising result found was that exercise intervention did not optimize the result of metformin treatment. The other finding of this study was that exercise intervention increased cholesterol and NOS level but exercise combined with metformin was not better than exercise alone. Thus, it appears from the present study that metformin and exercise combination has no effect.

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“…We injected pregnant female rats fed on HFD with STZ to induce GDM, which is a classic method for GDM induction (17). STZ injection improved the body weight and blood glucose, suggesting that GDM model was successfully constructed for further investigation.…”

Section: Discussionmentioning

confidence: 99%

Genetic variation of rs3811463 is associated with gestational diabetes mellitus susceptibility

Liu

Sun

et al. 2017

Exp Ther Med

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Abstract. Gestational diabetes mellitus (GDM) is a growing health concern, and it increases the risk of adverse pregnancy outcomes with substantial long-term adverse health impacts on mothers and their offspring. Several studies have revealed specific associations between genetic variants and the risk of GDM. Single nucleotide polymorphisms (SNPs) are the major type of genetic variation in humans. Let-7 microRNA targets are enriched for genes containing SNPs associated with glucose metabolism, including Lin28. In the present study, the effect of T/C variants of rs3811463 (a SNP located near to the let-7 binding site in Lin28) on GDM risk was investigated. A GDM rat model was successfully constructed using a high fat diet and streptozotocin injection, and the primary skeletal muscle cells were isolated. The cell transfection results demonstrated that rs3811463-T/C significantly affected the glucose uptake and insulin sensitivity. Reverse transcription-quantitative polymerase chain reaction analysis indicated that the C allele at rs3811463 regulated the expression of glucose metabolism-associated genes insulin-like growth factor two binding protein 2 and glucokinase. Western blot analysis data revealed that replacement of the T allele by the C allele at rs3811463 modulated the protein level of Sirtuin 1. Taken together, it was concluded that the let-7/Lin28 axis regulated glucose uptake and insulin sensitivity by modulating the expression of glucose metabolism-associated proteins. These findings provide novel evidence on the association between genetic variations of rs3811463 and GDM susceptibility.

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Successful abrogation by thymoquinone against induction of diabetes mellitus with streptozotocin via nitric oxide inhibitory mechanism

Cited by 65 publications

References 39 publications

Antidiabetic Properties of a Spice Plant Nigella sativa

Antidiabetic Properties of a Spice Plant Nigella sativa

The effect of metformin and exercise on serum lipids, nitric oxide synthase and liver nitric oxide levels in streptozotocin-nicotinamide induced diabetic rats

Genetic variation of rs3811463 is associated with gestational diabetes mellitus susceptibility

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