Successful 96-hour preservation of the canine pancreas

Abstract

We tested the preservation of the pancreas for 96 h by a modified two‐layer (UW solution/perfluorochemical) cold storage method (group 1) in the canine model of pancreas autotransplantation and compared this with an original two‐layer (Euro‐Collins' solution/perfluorochemical) cold storage method (group 2) and simple cold storage method with UW solution (group 3). A graft was considered functioning if the dog had a normal blood glucose for at least 5 days after transplantation. The functional success rates after preservation for 72 11 were 100%, 100% and 80% for groups 1, 2 and 3 respectively. On the other hand, the functional success rates for groups 1, 2 and 3 after preservation for 96 h were 75%, 0% and 0% respectively. The mean K value of 96‐hour preserved grafts for group 1 at 2 weeks after transplantation was 1.52 ± 0.30 compared with 1.98 ± 0.48 before preservation. Biopsies of grafts from group 1 showed almost normal pancreatic architecture even after preservation for 96 h. In addition, biopsies of grafts preserved for 9611 in group 1 at 4 weeks after transplantation showed almost normal endocrine tissue with mild fibrosis of the exocrine tissue. This study demonstrated the possibility of preserving the pancreas for 96 h prior to transplantation.

Preservation of Amputated Extremities by Extracorporeal Blood Perfusion; a Feasibility Study in a Porcine Model

“…The use of hemoglobin was thereafter abandoned and extremities were washed out [33,34], stored [35][36][37], perfused intermittently [34,38] or continuously [38][39][40] with fluids of different compositions, including physiological saline [41], Ringer Lactate [39], compositions using mannitol [42], and the standard organ preservation solutions University of Wisconsin [34,35,40,[43][44][45] and Euro Collins [33,39,43,45]. To increase the oxygenation of the perfused extremity, artificial oxygen carriers like Fluosol [36,38,46] and Fluorocarbon [38] have been used and one study even investigated the effect of hyperbaric oxygen chambers [47].…”

A Meta-Analysis for Comparison of the Two-Layer and University of Wisconsin Pancreas Preservation Methods in Islet Transplantation

“…There is direct evidence from the canine pancreas transplant model to show that ische-ment for short cold ischemic time (CIT) (21). During organ removal blood supply and hence oxygen is inter-mically damaged pancreata can be successfully resuscitated by TLM (5,8). rupted.…”

Chemical Oxygenation of Pancreatic Tissue Prior to Islet Isolation and Transplantation

“…Inspite of the amazing results that were obtained after autotransplantation of canine pancreases long-term stored for up to 72 and 96 hours (Kawamura et al, 1989, Kuroda et al, 1992c or exposed to 90 min of warm ischemia time (Kuroda et al, 1993a) only one study to perform TLM prior to clinical pancreas transplantation has been reported so far. Although an improvement in tissue quality and posttransplant function was observed, these differences were not significant when compared to storage in UWS (Matsumoto et al, 2000).…”