Success stories of natural product-derived compounds from plants as multidrug resistance modulators in microorganisms

Zhai, Xuguang; Wu, Guoyu; Tao, Xufeng; Yang, Shilei; Lv, Lei; Zhu, Yanna; Dong, Deshi; Xiang, Hong

doi:10.1039/d3ra00184a

Cited by 8 publications

(5 citation statements)

References 140 publications

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“…Success stories of natural product derivatives of medicinal plants or synthetic compounds have been investigated in several studies as multidrug-resistant modulators in bacteria by inhibiting or otherwise stopping their growth, according to a review by Zhai and collaborators [48]; for Campylobacter, we can highlight a review by Salimikia and collaborators that described active compounds of medicinal plants that are nature-based antibiotic agents that could be effective against Campylobacter [49]. However, there is an urgent need to coordinate efforts for meaningful research.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the Antibacterial Activity of Isatin against Campylobacter jejuni and Campylobacter coli Strains

Barroso,

Seki,

Esteves

et al. 2024

Applied Microbiology

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Antibiotic resistance, particularly against fluoroquinolones and macrolides, has emerged globally among thermophilic Campylobacters (Campylobacter jejuni and Campylobacter coli), giving rise to concerns about the efficacy of antibiotic treatment of these bacteria. Thus, developing new antibacterials with excellent activity is important. Isatin (IST) and its derivatives have exhibited promising antibacterial activities in several pathogenic bacteria. However, its activity against Campylobacter is unknown. Therefore, this study was conducted to evaluate the antibacterial activity of isatin against 29-Campylobacter strains (C. jejuni-17 and C. coli-12) and investigate the effects at the cellular level. The minimal inhibitory concentrations (MICs) of isatin were between <1.0 and 16.0 µg/mL in Campylobacter strains. Most strains presented with MIC = 8.0 µg/mL (76%). The minimal bactericidal concentration (MBC) was determined to be 16.0 µg/mL for 72% of the Campylobacter strains tested. The 50% inhibitory concentration (IC50) value for isatin was 125.63 µg/mL on the MRC-5 normal cell line, suggesting that isatin can be considered a safe substance in terms of cytotoxicity. In this study, we demonstrated the potential of isatin based on its low toxicity and effectiveness in vitro against antibiotic-resistant Campylobacter strains, which indicates that this compound could be an attractive candidate for future use in multidrug-resistant Campylobacter treatment.

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Section: Resultsmentioning

confidence: 99%

Evaluation of the Antibacterial Activity of Isatin against Campylobacter jejuni and Campylobacter coli Strains

Barroso,

Seki,

Esteves

et al. 2024

Applied Microbiology

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“…They inhibit biofilm formation, cell envelope synthesis, nucleic acid synthesis, and ATP synthesis and damage the bacterial respiratory chain, membrane bilayer, and membrane proteins [44]. As a terpenoid, glycyrrhizin's antibacterial activity is due to the disruption of membranes, anti-quorum sensing, and inhibition of protein and ATP synthesis [45]. However, major active compounds involved in the antibacterial activity of SO extract were not identified [46].…”

Section: Resultsmentioning

confidence: 99%

In Vitro Synergistic Inhibitory Effects of Plant Extract Combinations on Bacterial Growth of Methicillin-Resistant Staphylococcus aureus

Jeong,

Jung,

Yum

et al. 2023

Pharmaceuticals

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Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common pathogens of healthcare-associated infections. Medicinal plants have long been used in the traditional treatment of diseases or syndromes worldwide. Combined use of plant extracts could improve the effectiveness of pharmacological action by obtaining synergism, acting on multiple targets simultaneously, reducing the doses of individual components, and minimizing side effects. We aimed to investigate the synergistic inhibitory effects of selected medicinal plants (Caesalpinia sappan L. (CS), Glycyrrhiza uralensis Fisch. (GU), Sanguisorba officinalis L. (SO), and Uncaria gambir Roxb. (UG)) on the bacterial growth of MRSA and its clinical isolates. SO and UG extracts generated the best synergistic interaction as adjudged by checkerboard synergy assays. MICs of the individual extracts decreased 4-fold from 250 to 62.5 μg/mL, respectively. The SO + UG combination was further evaluated for its effects on bacterial growth inhibition, minimum bactericidal/inhibitory concentration (MBC/MIC) ratio, and time-kill kinetics. The results indicate that the SO + UG combination synergistically inhibited the bacterial growth of MRSA strains with bactericidal effects. SO + UG combination also exhibited more potent effects against clinical isolates. In multistep resistance selection experiments, both standard and isolates of MRSA showed no resistance to the SO + UG combination even after repeated exposure over fourteen passages. Our data suggest that using plant extract combinations could be a potential strategy to treat MRSA infections.

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“…The improper usage and overprescription of pharmaceuticals have led to MDR pathogens, which have become a serious global issue [79]. Structural modification of a drug molecule is one adaptive strategy employed by bacteria to develop resistance to drugs.…”

Section: Discussionmentioning

confidence: 99%

Metabolic Comparison and Molecular Networking of Antimicrobials in Streptomyces Species

Thapa,

Huo,

Budhathoki

et al. 2024

IJMS

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Streptomyces are well-known for producing bioactive secondary metabolites, with numerous antimicrobials essential to fight against infectious diseases. Globally, multidrug-resistant (MDR) microorganisms significantly challenge human and veterinary diseases. To tackle this issue, there is an urgent need for alternative antimicrobials. In the search for potent agents, we have isolated four Streptomyces species PC1, BT1, BT2, and BT3 from soils collected from various geographical regions of the Himalayan country Nepal, which were then identified based on morphology and 16S rRNA gene sequencing. The relationship of soil microbes with different Streptomyces species has been shown in phylogenetic trees. Antimicrobial potency of isolates was carried out against Staphylococcus aureus American Type Culture Collection (ATCC) 43300, Shigella sonnei ATCC 25931, Salmonella typhi ATCC 14028, Klebsiella pneumoniae ATCC 700603, and Escherichia coli ATCC 25922. Among them, Streptomyces species PC1 showed the highest zone of inhibition against tested pathogens. Furthermore, ethyl acetate extracts of shake flask fermentation of these Streptomyces strains were subjected to liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis for their metabolic comparison and Global Natural Products Social Molecular Networking (GNPS) web-based molecular networking. We found very similar metabolite composition in four strains, despite their geographical variation. In addition, we have identified thirty-seven metabolites using LC-MS/MS analysis, with the majority belonging to the diketopiperazine class. Among these, to the best of our knowledge, four metabolites, namely cyclo-(Ile-Ser), 2-n-hexyl-5-n-propylresorcinol, 3-[(6-methylpyrazin-2-yl) methyl]-1H-indole, and cyclo-(d-Leu-l-Trp), were detected for the first time in Streptomyces species. Besides these, other 23 metabolites including surfactin B, surfactin C, surfactin D, and valinomycin were identified with the help of GNPS-based molecular networking.

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Success stories of natural product-derived compounds from plants as multidrug resistance modulators in microorganisms

Abstract: Microorganisms evolve resistance to antibiotics as a function of evolution. Antibiotics have accelerated bacterial resistance through mutations and acquired resistance through a combination of factors.

Cited by 8 publications

References 140 publications

Evaluation of the Antibacterial Activity of Isatin against Campylobacter jejuni and Campylobacter coli Strains

Evaluation of the Antibacterial Activity of Isatin against Campylobacter jejuni and Campylobacter coli Strains

In Vitro Synergistic Inhibitory Effects of Plant Extract Combinations on Bacterial Growth of Methicillin-Resistant Staphylococcus aureus

Metabolic Comparison and Molecular Networking of Antimicrobials in Streptomyces Species

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