Subzero water transport characteristics and optimal rates of freezing rhesus monkey (<i>Macaca mulatta</i>) ovarian tissue

Li, Guohong; Thirumala, Sreedhar; Leibo, S.P.; Devireddy, Ram V.

doi:10.1002/mrd.20541

Cited by 15 publications

(5 citation statements)

References 67 publications

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“…For example, the permeability of equine ovarian tissues at subzero temperatures ranged from 0.01 to 0.12 ϫ 10 Ϫ14 m 3 /N-sec 27 and the corresponding parameters for macaque ovarian tissues ranged from 0.026 to 0.12 ϫ 10 Ϫ14 m 3 /N-sec. 28 Interestingly, the rate at which equine and macaque ovarian tissue sections are first cooled from ϩ25°to ϩ4°C has a significant effect on the membrane permeability to water when these tissues are subsequently frozen in molar concentrations of either glycerol, dimethylsulfoxide or ethylene glycol. 27,28 More importantly, the subzero permeability values are lower by a factor of 10 (or even 100) from the suprazero permeability values obtained in the present study (as shown in Tables 1 to 4).…”

Section: Ovine Follicle Permeability Parameters 195mentioning

confidence: 99%

“…28 Interestingly, the rate at which equine and macaque ovarian tissue sections are first cooled from ϩ25°to ϩ4°C has a significant effect on the membrane permeability to water when these tissues are subsequently frozen in molar concentrations of either glycerol, dimethylsulfoxide or ethylene glycol. 27,28 More importantly, the subzero permeability values are lower by a factor of 10 (or even 100) from the suprazero permeability values obtained in the present study (as shown in Tables 1 to 4). Although spermatozoa with their unique morphology differ substantially in size, shape and their surface area/volume ratio from ovarian follicles, we do note that analogous large differences between permeability coefficients at suprazero versus subzero temperatures have also been found for spermatozoa of several species.…”

Section: Ovine Follicle Permeability Parameters 195mentioning

confidence: 99%

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Permeability Characteristics of Ovine Primordial Follicles Calculated with Two Parameter Kedem-Katchalsky Formulation

Devireddy¹,

Amorim²

2006

Cell Preservation Technology

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The osmotic responses of isolated ovine primordial follicles when suspended in various concentrations of several cryoprotective additives (CPAs) have recently been published. We have used the Kedem-Katchalsky formulation, written with two parameters as variables, to calculate permeability of the follicle membrane to water, L p , and permeability to CPAs, . By fitting the model to the osmotic responses of follicles determined by Amorim and colleagues, we were able to calculate the permeability coefficients in the presence of 0.5, 1.0, 1.5, 2.0, or 2.5 M solutions of each of the following CPAs: dimethylsulfoxide (DMSO), ethylene glycol (EG), propylene glycol (PROH), and glycerol (GLY). In the presence of DMSO, the best-fit values of L p ranged from 0.15 to 0.29 ؋ 10 ؊14 m 3 /N-sec, and ranged from 0.30 to 0.50 ؋ 10 ؊11 mol/N-sec. In the presence of EG, the corresponding values of L p ranged from 0.25 to 0.47 ؋ 10 ؊14 m 3 /N-sec, and ranged from 0.20 to 0.65 ؋ 10 ؊11 mol/N-sec. In the presence of PROH, L p ranged from 0.10 to 0.62 ؋ 10 ؊14 m 3 /N-sec, and ranged from 0.10 to 0.40 ؋ 10 ؊11 mol/N-sec. And finally, in the presence of GLY, the best-fit values of L p ranged from 0.19 to 0.32 ؋ 10 ؊14 m 3 /N-sec, and ranged from 0.05 to 0.32 ؋ 10 ؊11 mol/N-sec. The cross correlation or the reflection coefficient, , defined as ‫؍‬ 1 ؊ , where v ෆCPA is the partial mole volume of the CPA, ranged from 0.763 to 0.926 in the presence of DMSO, from 0.870 to 0.977 in the presence of EG, from 0.813 to 0.983 in the presence of PROH, and from 0.918 to 0.988 in the presence of GLY. These values of L p and (and ) can be used to predict the volumetric response of ovine follicles under arbitrary CPA loading and unloading conditions and consequently, the presumptive optimal conditions. v ෆCPA ᎏ L p 188

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Section: Ovine Follicle Permeability Parameters 195mentioning

confidence: 99%

Section: Ovine Follicle Permeability Parameters 195mentioning

confidence: 99%

Permeability Characteristics of Ovine Primordial Follicles Calculated with Two Parameter Kedem-Katchalsky Formulation

Devireddy¹,

Amorim²

2006

Cell Preservation Technology

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“…The DSC has also been used to measure (a) ice nucleation parameters from intracellular ice formation (IIF) heat release readings in yeast and blood cells [ 27 ] and in G. Max cells and erythrocytes [ 28 ]; (b) heat releases associated with IIF in soybean cells [ 29 ], in lymphocytes [ 30 ] and in artemia cysts [ 31 ]; (c) the volume of freezable water in Drosophila melanogaster embryos [ 32 ]; (d) IIF heat release as a function of the cytocrit [ 33 ]; and (e) the phase change process in biologically relevant solutions [ 1 , 34 ]. In addition, the DSC has been used to measure the mass transfer (or water transport) across the cell membrane during the freezing of cells in suspension [ 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 ] and cells embedded in tissue [ 53 , 54 , 55 , 56 , 57 , 58 , 59 ].…”

Section: Introductionmentioning

confidence: 99%

Freezing of Solute-Laden Aqueous Solutions: Kinetics of Crystallization and Heat- and Mass-Transfer-Limited Model

et al. 2022

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Following an earlier study, we reexamined the latent heat of fusion during freezing at 5 K/min of twelve different pre-nucleated solute-laden aqueous solutions using a Differential Scanning Calorimeter (DSC) and correlated it with the amount of initially dissolved solids or solutes in the solution. In general, a decrease in DSC-measured heat release (in comparison to that of pure water, 335 mJ/mg) was observed with an increasing fraction of dissolved solids or solutes, as observed in the earlier study. In addition, the kinetics of ice crystallization was also obtained in three representative biological media by performing additional experiments at 1, 5 and 20 K/min. A model of ice crystallization based on the phase diagram of a water–NaCl binary solution and a modified Avrami-like model of kinetics was then developed and fit to the experimental data. Concurrently, a heat and mass transfer model of the freezing of a salt solution in a small container is also presented to account for the effect of the cooling rate as well as the solute concentration on the measured latent of freezing. This diffusion-based model of heat and mass transfer was non-dimensionalized, solved using a numerical scheme and compared with experimental results. The simulation results show that the heat and mass transfer model can predict (± 10%) the experimental results.

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“…A method of direct measurement of L p at subzero temperatures using differential scanning calorimetry (DSC) was proposed by Devireddy et al 21 Later, this method was applied for the measurements of cryobiological properties of both cells and tissues. [22][23][24][25][26][27][28] In this study, we used this method to investigate the temperature-dependent cell membrane permeability to water for human vaginal mucosal T cells and macrophages. Based on the results, we predicted the theoretically optimal cooling rates for the immune cells and tested those rates in preliminary cryopreservation experiments.…”

Section: Introductionmentioning

confidence: 99%

Determination of the Membrane Permeability to Water of Human Vaginal Mucosal Immune Cells at Subzero Temperatures Using Differential Scanning Calorimetry

Shu

Hughes

Fang

et al. 2016

Biopreservation and Biobanking

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To study mucosal immunity and conduct HIV vaccine trials, it is important to be able to cryopreserve mucosal specimens and recover them in functional viable form. Obtaining a good recovery depends, in part, on cooling the cells at the appropriate rate, which is determined by the rate of water transport across the cell membrane during the cooling process. In this study, the cell membrane permeabilities to water at subzero temperatures of human vaginal mucosal T cells and macrophages were measured using the differential scanning calorimetry method proposed by Devireddy et al. in 1998. Thermal histograms were measured before and after cell lysis using a Slow-Fast-Fast-Slow cooling program. The difference between the thermal histograms of the live intact cells and the dead lysed cells was used to calculate the temperature-dependent cell membrane permeability at subzero temperatures, which was assumed to follow the Arrhenius relationship, [Formula: see text], where Lpg is the permeability to water at the reference temperature (273.15 K). The results showed that Lpg = 0.0209 ± 0.0108 μm/atm/min and Ea = 41.5 ± 11.4 kcal/mol for T cells and Lpg = 0.0198 ± 0.0102 μm/atm/min and Ea = 38.2 ± 10.4 kcal/mol for macrophages, respectively, in the range 0°C to -40°C (mean ± standard deviation). Theoretical simulations predicted that the optimal cooling rate for both T cells and macrophages was about -3°C/min, which was proven by preliminary immune cell cryopreservation experiments.

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Subzero water transport characteristics and optimal rates of freezing rhesus monkey (Macaca mulatta) ovarian tissue

Cited by 15 publications

References 67 publications

Permeability Characteristics of Ovine Primordial Follicles Calculated with Two Parameter Kedem-Katchalsky Formulation

Permeability Characteristics of Ovine Primordial Follicles Calculated with Two Parameter Kedem-Katchalsky Formulation

Freezing of Solute-Laden Aqueous Solutions: Kinetics of Crystallization and Heat- and Mass-Transfer-Limited Model

Determination of the Membrane Permeability to Water of Human Vaginal Mucosal Immune Cells at Subzero Temperatures Using Differential Scanning Calorimetry

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