Subwavelength imaging through ion-beam-induced upconversion

Mi, Zhaohong; Zhang, Yuhai; Vanga, Sudheer Kumar; Chen, Ce-Belle; Tan, Hong Qi; Watt, F.; Liu, Xiaogang; Bettiol, Andrew A.

doi:10.1038/ncomms9832

Cited by 39 publications

(27 citation statements)

References 38 publications

(49 reference statements)

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“…The mechanisms can be ion beam-induced photoluminescence or upconversion. For example, with incubated NdYF 4 :Yb/Tm nanoparticles, Mi et al reported imaging resolution of 28 nm for cellular structures, which was significantly beyond what was attained (~253 nm) by using a 980 nm laser optical probe [114].…”

Section: Focused Helium Ion Beams For 3d Nano Imagingmentioning

confidence: 96%

Tipping solutions: emerging 3D nano-fabrication/ -imaging technologies

et al. 2017

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The evolution of optical microscopy from an imaging technique into a tool for materials modification and fabrication is now being repeated with other characterization techniques, including scanning electron microscopy (SEM), focused ion beam (FIB) milling/imaging, and atomic force microscopy (AFM). Fabrication and in situ imaging of materials undergoing a three-dimensional (3D) nano-structuring within a 1−100 nm resolution window is required for future manufacturing of devices. This level of precision is critically in enabling the cross-over between different device platforms (e.g. from electronics to micro-/ nano-fluidics and/or photonics) within future devices that will be interfacing with biological and molecular systems in a 3D fashion. Prospective trends in electron, ion, and nano-tip based fabrication techniques are presented.

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Section: Focused Helium Ion Beams For 3d Nano Imagingmentioning

confidence: 96%

Tipping solutions: emerging 3D nano-fabrication/ -imaging technologies

et al. 2017

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“…Another possibility is to find other excitation sources that are not limited to an optical excitation source, for lanthanide doped nanoparticles. A recent study on helium ion beam excited upconversion luminescence sheds light on the exploration of a new excitation source . Thus, new excitation sources not only can provide alternative excitation options, but also can render new features to upconversion nanoparticles, like upconversion nanoparticle based subwavelength imaging.…”

Section: Summary and Perspectivesmentioning

confidence: 99%

Emerging ≈800 nm Excited Lanthanide‐Doped Upconversion Nanoparticles

Xie

Zhang

et al. 2016

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Lanthanide-doped upconversion nanoparticles can tune near-infrared light to visible or even ultra-violet light in emissions. Due to their unique photophysical and photochemical properties, as well as their promising bioapplications, there has been a great deal of enthusiastic research performed to study the properties of lanthanide-doped upconversion nanoparticles in the past few years. Despite the considerable progress in this area, numerous challenges associated with the nanoparticles, such as a low upconversion efficiency, limited host materials, and a confined excitation wavelength, still remain, thus hindering further development with respect to their applications and in fundamental science. Recently, innovative strategies that utilize alternative sensitizers have been designed in order to engineer the excitation wavelengths of upconversion nanoparticles. Here, focusing on the excitation wavelength at ≈800 nm, recent advances in the design, property tuning, and applications of ≈800 nm excited upconversion nanoparticles are summarized. Benefiting from the unique features of ≈800 nm light, including deep tissue penetration depth and low photothermal effect, the ≈800 nm excited upconversion nanoparticles exhibit superior potential for biosensing, bioimaging, drug delivery, therapy, and three dimensional displays. The critical aspects of such emerging nanoparticles with regards to meeting the ever-changing needs of future development are also discussed.

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“…As such, the sub-30 nm spatial resolution can be maintained throughout the cell for whole-cell imaging, in stark contrast with electron microscopy. Moreover, a highly-focused beam of MeV helium ions allows imaging of cell structure by measuring ion energy loss in the scanning transmissionion mode 9,10 , whilst simultaneously performing luminescence imaging by collecting luminescent photons upon excitation by helium ions 11 , at super resolutions. Therefore, we hypothesize that a correlative iono-nanoscopic approach can be implemented for whole-cell luminescence and ultrastructure mapping in a single imaging experiment.…”

mentioning

confidence: 99%

Quantifying nanodiamonds biodistribution in whole cells with correlative iono-nanoscopy

Tan

Chen

et al. 2020

Preprint

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Correlative imaging and quantification of intracellular nanoparticles with the underlying ultrastructure is crucial for understanding cell-nanoparticle interactions in biological research. However, correlative nanoscale imaging of whole cells still remains a daunting challenge. Here, we report a straightforward nanoscopic approach for whole-cell correlative imaging, by simultaneous ionoluminescence and ultrastructure mapping implemented with a highly focused beam of alpha particles. We demonstrate that fluorescent nanodiamonds exhibit fast, ultrabright and stable emission upon excitation by alpha particles. Thus, by using fluorescent nanodiamonds as imaging probes, our approach enables quantification and correlative localization of single nanodiamonds within a whole cell at sub-40 nm resolution. As an application example, we show that our approach, together with Monte Carlo simulations, can be employed to provide unique insights into the mechanisms of nanodiamond radiosensitization at the single whole-cell level. These findings may benefit clinical studies of radio-enhancement effects by nanoparticles in charged-particle cancer therapy.

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Subwavelength imaging through ion-beam-induced upconversion

Cited by 39 publications

References 38 publications

Tipping solutions: emerging 3D nano-fabrication/ -imaging technologies

Tipping solutions: emerging 3D nano-fabrication/ -imaging technologies

Emerging ≈800 nm Excited Lanthanide‐Doped Upconversion Nanoparticles

Quantifying nanodiamonds biodistribution in whole cells with correlative iono-nanoscopy

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