Subunit association, and thermal and chemical unfolding of Cucurbitaceae phloem exudate lectins. A review

Swamy, Musti J.; Mondal, Saradamoni

doi:10.1016/j.ijbiomac.2023.123434

Cited by 4 publications

(4 citation statements)

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“…PP2 proteins are involved in wound healing, resistance to biotic stress, and nutrient transport in plants. It is speculated that the FBP subfamily could be involved in regulating the formation of phloem in plants and participating in nutrient transport pathways [50,51]. The LRR (leucine-rich repeats) domain has been confirmed to be involved in plant root development and immunity in Arabidopsis thaliana [52].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification, Characterization and Expression Profile of F-Box Protein Family Genes Shed Light on Lateral Branch Development in Cultivated Peanut (Arachis hypogaea L.)

Li,

Guo,

Wang

et al. 2024

Horticulturae

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F-box proteins are a large gene family in plants, and play crucial roles in plant growth, development, and stress response. To date, a comprehensive investigation of F-box family genes in peanuts, and their expression pattern in lateral branch development has not been performed. In this study, a total of 95 F-box protein family members on 18 chromosomes, named AhFBX1-AhFBX95, were identified in cultivated peanut (Arachis hypogaea L.), which were classified into four groups (Group I–IV). The gene structures and protein motifs of these peanut FBX genes were highly conserved among most FBXs. We found that significant segmental duplication events occurred between wild diploid species and the allotetraploid of peanut FBXs, and observed that AhFBXs underwent strong purifying selection throughout evolution. Cis-acting elements related to development, hormones, and stresses were identified in the promoters of AhFBX genes. In silico analysis of AhFBX genes revealed expression patterns across 22 different tissues. A total of 32 genes were predominantly expressed in leaves, pistils, and the aerial gynophore tip. Additionally, 37 genes displayed tissue-specific expression specifically at the apex of both vegetative and reproductive shoots. During our analysis of transcriptome data for lateral branch development in spreading and erect varieties, namely M130 and JH5, we identified nine deferentially expressed genes (DEGs). Quantitative real-time PCR (qRT-PCR) results further confirmed the expression patterns of these DEGs. These DEGs exhibited significant differences in their expression levels at different stages between M130 and JH5, suggesting their potential involvement in the regulation of lateral branch development. This systematic research offers valuable insights into the functional dissection of AhFBX genes in regulating plant growth habit in peanut.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification, Characterization and Expression Profile of F-Box Protein Family Genes Shed Light on Lateral Branch Development in Cultivated Peanut (Arachis hypogaea L.)

Li,

Guo,

Wang

et al. 2024

Horticulturae

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“…It was observed that 100-Leu-Ile-Glu-Val-Ser-Trp-105 and 199-Trp-Lys-200 amino acid residues were highly conserved among these proteins and responsible for binding to chitooligosaccharides. The functional roles of CPELs in plants included chitin-binding properties, anti-pathogenic response, wound-sealing, RNA-binding and transport as well as molecular chaperone-like activity in the stress response [ 11 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…In this study, an acetylated chitin column was used to purify the Cucurbita pepo lectin (denoted as CPL) with an estimated molecular weight of 30 ± 1 kDa. Swamy et al and others reported CPELs (Cucurbita phloem exudate lectins) to be homodimeric proteins with subunits of ~16–26 kDa molecular mass proteins [ 11 , 16 , 17 , 18 , 19 , 20 ]. Phloem exudate proteins with various molecular weights were purified long ago from Cucumis melo , Cucumis sativus and Cucurbita maxima [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Carbohydrate-Binding Properties and Antimicrobial and Anticancer Potential of a New Lectin from the Phloem Sap of Cucurbita pepo

Islam,

Hossain,

Khanam

et al. 2024

Molecules

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A Cucurbita phloem exudate lectin (CPL) from summer squash (Cucurbita pepo) fruits was isolated and its sugar-binding properties and biological activities were studied. The lectin was purified by affinity chromatography and the hemagglutination assay method was used to determine its pH, heat stability, metal-dependency and sugar specificity. Antimicrobial and anticancer activities were also studied by disc diffusion assays and in vivo and in vitro methods. The molecular weight of CPL was 30 ± 1 KDa and it was stable at different pH (5.0 to 9.0) and temperatures (30 to 60 °C). CPL recovered its hemagglutination activity in the presence of Ca2+. 4-nitrophenyl-α-d-glucopyranoside, lactose, rhamnose and N-acetyl-d-glucosamine strongly inhibited the activity. With an LC50 value of 265 µg/mL, CPL was moderately toxic and exhibited bacteriostatic, bactericidal and antibiofilm activities against different pathogenic bacteria. It also exhibited marked antifungal activity against Aspergillus niger and agglutinated A. flavus spores. In vivo antiproliferative activity against Ehrlich ascites carcinoma (EAC) cells in Swiss albino mice was observed when CPL exerted 36.44% and 66.66% growth inhibition at doses of 3.0 mg/kg/day and 6.0 mg/kg/day, respectively. A 12-day treatment by CPL could reverse their RBC and WBC counts as well as restore the hemoglobin percentage to normal levels. The MTT assay of CPL performed against human breast (MCF-7) and lung (A-549) cancer cell lines showed 29.53% and 18.30% of inhibitory activity at concentrations of 128 and 256 µg/mL, respectively.

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The crystal structure of Nictaba reveals its carbohydrate-binding properties and a new lectin dimerization mode

Bloch,

Osterne,

Savvides

et al. 2024

Preprint

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Nictaba is a (GlcNAc)n-binding, stress-inducible lectin fromNicotiana tabacum, that serves as the archetypical lectin for the family of Nictaba-related lectins. Nictaba and Nictaba-related lectins play pivotal roles in plant defense mechanisms and stress response pathways. Despite extensive research into the variety of biological activities and physiological role(s) of the lectin, the three-dimensional structure of Nictaba remained largely unknown. Here, we report crystal structures for Nictaba in the apo form and bound to chitotriose. The structures reveal a jelly-roll fold for the Nictaba protomer and the assembly thereof into a novel dimerization interface among lectins. The chitotriose binding mode centers around the central GlcNAc residue providing insights into the determinants of specificity of Nictaba towards carbohydrate structures. Indeed, by integrating such structural insights with inputs from glycan arrays, molecular docking, and molecular dynamics simulations, we propose that Nictaba employs a single carbohydrate-recognition domain within each of the two subunits in the dimer to display pronounced specificity towards GlcNAc-containing carbohydrates. Furthermore, we identified amino acid residues involved in the extended binding site capable of accommodating structurally diverse high-mannose and complexN-glycans, highlighting the lectin potential to recognizeN-glycan structures. Glycan array andin silicoanalyses revealed interactions centered around the conserved Man3GlcNAc2 core, explaining the broad recognition ofN-glycan structures. Collectively, the integrated structural and biochemical insights presented here fill a hitherto substantial void into the atlas of lectin structure-function relationships and pave the way for future developments in plant stress biology and lectin-based applications.

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Subunit association, and thermal and chemical unfolding of Cucurbitaceae phloem exudate lectins. A review

Cited by 4 publications

References 64 publications

Genome-Wide Identification, Characterization and Expression Profile of F-Box Protein Family Genes Shed Light on Lateral Branch Development in Cultivated Peanut (Arachis hypogaea L.)

Genome-Wide Identification, Characterization and Expression Profile of F-Box Protein Family Genes Shed Light on Lateral Branch Development in Cultivated Peanut (Arachis hypogaea L.)

Carbohydrate-Binding Properties and Antimicrobial and Anticancer Potential of a New Lectin from the Phloem Sap of Cucurbita pepo

The crystal structure of Nictaba reveals its carbohydrate-binding properties and a new lectin dimerization mode

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