Subtyping of Streptococcus uberis by DNA amplification fingerprinting

Jayarao, Bhushan M.; Bassam, Brant J.; Caetano-Anollés, G; Gresshoff, Peter M.; Oliver, S. P.

doi:10.1128/jcm.30.5.1347-1350.1992

Cited by 67 publications

(25 citation statements)

References 18 publications

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“…Based on these findings, the likelihood of a quarter becoming clinical decreases with increasing days in milk and lactation age. Previous studies on the molecular epidemiology of S. uberis based on DNA fingerprinting suggested that most S. uberis isolates of clinical mastitis origin were also isolated from cows with subclinical mastitis (JAYARAO et al, 1991b(JAYARAO et al, , 1992(JAYARAO et al, , 1993. On most occasions, S. uberis clinical mastitis during early lactation was preceded by a variable length of subclinical mastitis caused by the same DNA fingerprint type.…”

Section: Resultsmentioning

confidence: 96%

Epidemiology of Streptococcus uberis Intramammary Infections in a Dairy Herd

Jayarao

Gillespie

Lewis

et al. 1999

Journal of Veterinary Medicine, Series B

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From 1987 to 1991, almost 36,000 quarter samples of mammary secretion representing 1790 lactations of 510 dairy cows from a research herd were collected for bacteriological examination. The percentage of cows infected with Streptococcus uberis ranged from 12 to 16% of cows/year. S. uberis was isolated from 14.2% of lactations over the 5-year period. The prevalence of S. uberis intramammary infection (IMI) was significantly higher in cows with > or = 4 lactations than in cows with 3 or fewer lactations. Regardless of lactation number, the prevalence of S. uberis was highest before parturition, during early lactation and near drying off. The prevalence of S. uberis infected quarters ranged from 1.3 to 2.3% of quarters/year; the prevalence rate for the 5-year period was 2% of quarters. The quarter prevalence of S. uberis was lowest in cows with < or = 3 lactations, increased significantly with lactation number and was highest in cows with > or = 6 lactations. The percentage of quarters infected with S. uberis varied significantly by year. The majority (95%) of S. uberis IMI were subclinical. The ratio of subclinical IMI to clinical IMI was lowest during early lactation, and increased with days in milk, and with lactation age except for cows in their 5th and 6th lactations. Results of this epidemiological investigation suggest that opportunities exist where suitable control measures could be applied to reduce the impact of S. uberis infections in the dairy herd.

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Section: Resultsmentioning

confidence: 96%

Epidemiology of Streptococcus uberis Intramammary Infections in a Dairy Herd

Jayarao

Gillespie

Lewis

et al. 1999

Journal of Veterinary Medicine, Series B

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“…Streptococcus species [1,3,9], and Staphylococcus species [2] isolated from bovine mammary secretions. Results of those studies demonstrated clearly that PCR-based DNA ¢ngerprinting was an accurate procedure for identi¢cation of mastitis pathogens.…”

Section: Resultsmentioning

confidence: 99%

“…DNA ¢ngerprint pro¢les were speci¢c for each bacterial species evaluated, however, subtle di¡erences were observed suggesting that this procedure might be useful to detect di¡erences among strains of a species. Subsequent studies evaluated PCR-based DNA ¢ngerprinting as a method of subtyping S. uberis [3], S. dysgalactiae [4], E. faecium [5] and S. aureus [6] with encouraging results.Marked clonal diversity among strains of S. uberis isolated from cows has been reported [9,10]. Of 50 strains of S. uberis evaluated, 35 DNA ¢ngerprint patterns and eight distinct clusters were observed [10].…”

Section: Resultsmentioning

confidence: 99%

“…We reported results of PCR-based DNA ¢ngerprinting that in-volves ampli¢cation of bacterial genomic DNA by a short oligonucleotide primer to produce a distinct pattern of ampli¢ed DNA fragments [1,2]. This procedure has been used for identi¢cation of Streptococcus, Enterococcus, and Staphylococcus species from bovine milk [1,2] and as a method of subtyping S. uberis [3,4], S. dysgalactiae [4], Enterococcus faecium [5] and Staphylococcus aureus [6]. The objective of the present study was to use PCR-based DNA ¢ngerprinting as a tool to di¡erentiate new from persistent S. uberis and S. dysgalactiae intramammary infections (IMI) in dairy cows.…”

Section: Introductionmentioning

confidence: 99%

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Detection of new and persistent Streptococcus uberis and Streptococcus dysgalactiae intramammary infections by polymerase chain reaction-based DNA fingerprinting

1998

FEMS Microbiology Letters

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Polymerase chain reaction-based DNA fingerprinting was used as a tool to differentiate new and persistent Streptococcus uberis and Streptococcus dysgalactiae intramammary infections (IMI) in dairy cows. The same subtype of S. uberis or S. dysgalactiae was detected from some infected mammary glands from one lactation to the next documenting the persistence of these infections. Conversely, some streptococci isolated from mammary glands during a lactation or from one lactation to the next were different subtypes suggesting that a new IMI occurred. These new streptococcal IMI would never have been detected using phenotypic methods of streptococcal identification. Results of this study indicate that PCR-based DNA fingerprinting can be used as an effective procedure to differentiate new and persistent S. uberis and S. dysgalactiae IMI in dairy cows. This technique will be useful in epidemiological investigations, and drug and vaccine efficacy studies when attempting to delineate new and persistent IMI. z

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“…However, detection of polymorphisms by techniques involving hybridization to Southern blots is time-consuming and laborious. Recently, simpler methods have been reported for assessing DNA polymorphisms by amplifying genomic DNA with single primers of arbitrary nucleotide sequences [20, 211. Polymorphisms generated by this method have been termed arbitrarily primed-polynierase chain reaction (AP-PCR) fingerprinting [20], random amplification of polymorphic DNA (RAPD) markers [2 l], and DNA amplification fingerprinting (DAF) [22]. This use of the PCR with arbitrary primers differing in length and nucleotide composition detected polymorphisms in the absence of specific nucleotide sequence information in DNA from bacteria, fungi, plants, humans and other animals [20-251.…”

Section: Introductionmentioning

confidence: 99%

Identification of clinical strains of Candida albicans by DNA fingerprinting with the polymerase chain reaction

Schönian

Meusel

Tietz

et al. 1993

Mycoses

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Summary. DNA polymorphisms generated by the polymerase chain reaction (PCR) were used to differentiate clinical isolates of Candida. This PCR method employed single primers that were originally designed as hybridization probes for DNA fingerprinting experiments to probe mini‐satellite and microsatellite DNA sequences. To evaluate this procedure, 35 isolates from 20 patients in several intensive care units and 12 isolates obtained from the oral cavities of healthy dental patients were fingerprinted. The PCR‐fingerprint patterns of isolates of Candida albicans from the immunocompromised patients revealed fewer differences than isolates from the dental service. Multiple isolates from different body sites of the same patients revealed that patients may harbour isolates of Candida with the same or different PCR‐fingerprints. Since this method is generally simpler and faster than established methods of biotyping medically important yeasts, PCR‐fingerprinting may prove useful for the surveying of large numbers of pathogens for epidemi‐ological studies. Zusammenfassung. Mittels PCR erhaltene DNA‐Polymorphismen wurden zur Differen‐zierung von klinischen Candida‐Isolaten einge‐setzt. Einzelne Primer, welche spezifisch Mini‐ und Mikrosatelliten‐DNA‐Sequenzen amplifizieren, wurden fur das PCR‐Fingerprinting genutzt. Mit dieser Methode wurden 35 Isolate, welche von 20 Patienten ver‐schiedener Intensivtherapiestationen stammten, sowie 12 Isolate aus der Mundhöhle gesunder stomatologischer Patienten untersucht. Die PCR‐Fingerprint‐Muster der Candida‐Stämme, die von immunsupprimierten Patienten isoliert wurden, zeigten dabei geringere Unterschiede als die der Oralisolate. Isolate von verschiedenen Körperstellen des gleichen Patienten ergaben entweder identische oder differierende PCR‐Fingerprints. Die PCR ist in vielen Fällen leichter und schneller durchführbar als andere, bereits etablierte, genetische Verfahren für die Typisierung von Krankheitserregern. Für die Untersuchung einer großen Anzahl von Isolaten im Rahmen epidemiologischer Studien ist diese Methode deshalb von besonderem Wert.

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Subtyping of Streptococcus uberis by DNA amplification fingerprinting

Cited by 67 publications

References 18 publications

Epidemiology of Streptococcus uberis Intramammary Infections in a Dairy Herd

Epidemiology of Streptococcus uberis Intramammary Infections in a Dairy Herd

Detection of new and persistent Streptococcus uberis and Streptococcus dysgalactiae intramammary infections by polymerase chain reaction-based DNA fingerprinting

Identification of clinical strains of Candida albicans by DNA fingerprinting with the polymerase chain reaction

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