Subtype distribution of Blastocystis spp. isolated from children in Eskisehir, Turkey

Doğan, Nihal; Aydın, Merve; Tüzemen, Nazmiye Ülkü; Dinleyici, Ener Çağrı; Oguz, Ilkiz; Doğruman-Al, Funda

doi:10.1016/j.parint.2016.10.008

Cited by 33 publications

(9 citation statements)

References 39 publications

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“…We found Blastocystis subtypes 1, 2 and 3, where Blastocystis subtype 3 was the most prevalent followed by subtype 1 and 2. Similar distribution and prevalence have been reported in South America (10,15,29) and some places around the world (4,30,31). However, other studies from Brazil, and Lao Democratic Republic shows subtype 1 is the most prevalent (32,33), while in Europe the most prevalent subtypes are 3 and 4 (34,35).…”

Section: Discussionsupporting

confidence: 83%

“…In our study we used a much larger sample size and identified subtypes 1, 2, 3 and unknown subtypes. Mixed-double infections between Blastocystis subtype 1, 2 and 3 with different proportions and combinations have been reported in Brazil, Turkey and Iran (4,30,32). In our result we report 8.6% mixed-double subtypes infections corresponding to subtypes 1 and 3.…”

Section: Discussionmentioning

confidence: 99%

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Molecular epidemiology of Blastocystis in urban and periurban human populations in Arequipa, Peru

Ascuña-Durand

Salazar-Sánchez

Cartillo-Neyra

et al. 2020

Preprint

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Blastocystis is one of the most common parasites found in the gut of humans and other hosts. It has a wide genetic diversity distributed around the world, predominating subtypes 1, 2 and 3 in South America countries. Nonspecific and inconsistent symptoms associated with this parasite made it controversial. The aim of this study was to estimate the prevalence of subtypes and determinate the epidemiological conditions associated with them. A total of 116 Blastocystis positive stool samples were processed by conventional PCR with Blastocystis-specific primers for subtypes 1 to 3. We identified subtype 1 (10.3%), subtype 2 (7.6%), subtype 3 (25.0%) and mixed infections (8.6%). We did not identify these Blastocystis subtypes in 48.7% of stool samples, assuming the presence of other subtypes in the zone. Any association was found between gastrointestinal symptoms and single subtype infections neither with mixed subtypes coinfections. However, our results suggest an association of Blastocystis subtype 2 and Irritable Bowel Syndrome (IBS, p=0.039). Besides, there was not an association between Blastocystis subtypes 1, 2 and 3 nor-mixed infections with epidemiological variables such as gender, age, presence of animals or vectors, places of food consumption, type of water consumption and water supply.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of Blastocystis in urban and periurban human populations in Arequipa, Peru

Ascuña-Durand

Salazar-Sánchez

Cartillo-Neyra

et al. 2020

Preprint

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“…In our study, ST4, which is generally found across Europe, was detected in only one immunocompetent patient. In Turkey, reports in humans of ST4 are sporadic ( S4 Table ) [ 86 , 118 , 123 ]. In our study, ST6 was detected in one immunocompetent and one immunodeficient patient.…”

Section: Discussionmentioning

confidence: 99%

Investigation of neglected protists Blastocystis sp. and Dientamoeba fragilis in immunocompetent and immunodeficient diarrheal patients using both conventional and molecular methods

et al. 2021

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Introduction The clinical significance of Blastocystis sp. and Dientamoeba fragilis in patients with gastrointestinal symptoms is a controversial issue. Since the pathogenicity of these protists has not been fully elucidated, testing for these organisms is not routinely pursued by most laboratories and clinicians. Thus, the prevalence of these organisms and the subtypes of Blastocystis sp. in human patients in Turkey are not well characterized. This study aimed to determine the prevalence of Blastocystis sp. and D. fragilis in the diarrheic stool samples of immunodeficient and immunocompetent patients using conventional and molecular methods and to identify Blastocystis sp. subtypes using next generation sequencing. Material and methods Individual stool specimens were collected from 245 immunodeficient and 193 immunocompetent diarrheic patients between March 2017 and December 2019 at the Gazi University Training and Research Hospital in Ankara, Turkey. Samples were screened for Blastocystis sp. and D. fragilis by conventional and molecular methods. Molecular detection of both protists was achieved by separate qPCRs targeting a partial fragment of the SSU rRNA gene. Next generation sequencing was used to identify Blastocystis sp. subtypes. Results The prevalence of Blastocystis sp. and D. fragilis was 16.7% and 11.9%, respectively as measured by qPCR. The prevalence of Blastocystis sp. and D. fragilis was lower in immunodeficient patients (12.7% and 10.6%, respectively) compared to immunocompetent patients (21.8% and 13.5%, respectively). Five Blastocystis sp. subtypes were identified and the following subtype distribution was observed: ST3 54.4% (n = 37), ST2 16.2% (n = 11), ST1 4.4% (n = 3), ST6 2.9% (n = 2), ST4 1.5% (n = 1), ST2/ST3 11.8% (n = 8) and ST1/ST3 8.8% (n = 6). There was no statistically significant difference in the distribution of Blastocystis sp. subtypes between immunocompetent and immunodeficient patients. Conclusion and recommendation Our findings demonstrated that Blastocystis sp. and D. fragilis are commonly present in immunocompetent and immunodeficient patients with diarrhea. This study is the first to use next generation sequencing to address the presence of Blastocystis sp. mixed subtypes and intra-subtype variability in clinical samples in Turkey.

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“…It is noted that hematologic and non-hematologic (cranial) cancers with Blastocystis spp. infections are most commonly reported in children [82][83][84][85]. Whereas, colorectal, stomach, esophagus and non-gastrointestinal cancer such as lung, liver, breast, ovarian, hematologic and other cancers were detected in adults.…”

Section: Blastocystis Spp Infection In Humansmentioning

confidence: 99%

The Coexistence of Blastocystis spp. in Humans, Animals and Environmental Sources from 2010–2021 in Asia

Rauff-Adedotun

Termizi

Shaari

et al. 2021

Biology

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Blastocystis spp. are controversial unicellular protists that inhabit the gastrointestinal tract of humans and a wide range of animals worldwide. This review provides an overview of the prevalence and distribution of Blastocystis spp. and their subtypes throughout Asia. Research articles reporting on the presence of Blastocystis spp. in locations within Asia, between 1 January 2010, and 10 May 2021, were obtained from Scopus, PubMed, and Google Scholar. In 427 articles, the prevalence of Blastocystis spp. in 31 countries within the last decade was revealed. Isolates were found in humans, various mammals, birds, reptiles, insects, water sources, vegetables, and ambient air. Prevalence of Blastocystis spp. varied widely across host categories. Subtypes identified throughout Asia were STs 1–14, and ST18–22 (novel subtypes). ST1, ST2, ST3, ST4 were the most frequently isolated in humans; ST5 in pigs; ST10 and ST14 in goats, sheep, and cattle; and ST6 and ST7 in chickens. ST1 and ST3 were most common in water samples. ST1, ST2, ST3, ST4, ST5 and ST6 were shared by humans, animals, and water sources. There is a growing interest in the study of Blastocystis spp. and their subtypes in Asia. Due to the isolation of Blastocystis spp. from biotic and abiotic sources in Asia, the application of the One Health (OH) approach to the study of Blastocystis spp. is proposed for improved perception of this organism.

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Subtype distribution of Blastocystis spp. isolated from children in Eskisehir, Turkey

Cited by 33 publications

References 39 publications

Molecular epidemiology of Blastocystis in urban and periurban human populations in Arequipa, Peru

Molecular epidemiology of Blastocystis in urban and periurban human populations in Arequipa, Peru

Investigation of neglected protists Blastocystis sp. and Dientamoeba fragilis in immunocompetent and immunodeficient diarrheal patients using both conventional and molecular methods

The Coexistence of Blastocystis spp. in Humans, Animals and Environmental Sources from 2010–2021 in Asia

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