Subtrochanteric Fracture as First Sign of Metastatic Breast Cancer: Case Presentation

Deleanu, Bogdan; Prejbeanu, Radu; Crişan, Dan; Vermeşan, Dinu; Predescu, Vlad; Iacob, Mihaela; Dema, Alis

doi:10.14740/jmc2216w

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…These correlations should be well studied in the future, to be able to observe if exists an association between these pathologies and also an etiological pathway that could be interrupted by preventive therapies. Some authors identified a correlation between Paget's disease of bone and osteosclerosis [32][33][34][35][36][37], condition that was also found in a 45-year-old female patient from present paper, but the association of these two diseases must be furthermore investigated.…”

Section:  Discussionmentioning

confidence: 71%

Otosclerosis under the magnifying glass

Vîrzob¹,

Cloşca²,

Poenaru³

et al. 2023

Rom J Morphol Embryol

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Otosclerosis is a bone condition affecting the stapes bone within the otic capsule, and its exact cause is still unknown. It is characterized by a lack of proper remodeling of newly formed vascular and woven bone, leading to the development of abnormal osteons and the formation of sclerotic bone. Bilateral otosclerosis is seen in 80% of patients and 60% of otosclerosis patients have a family history of the condition. The etiology of this disease is still unknown, there are lots of theories to explain it. The histopathological (HP) studies of otosclerosis showed that osteoblasts, osteoclasts, vascular proliferation, fibroblasts, and histiocytes were observed in the stapes footplate. The onset of the symptoms occurs by the early third decade of life, usually it doesn’t start later. In otosclerosis, the energy exerted by sound at the level of the tympanic membrane is reduced in the inner ear due to the fixation and rigidity of the ossicular chain, leading to hearing loss, especially for low frequencies. The primary clinical symptom of otosclerosis is conductive hearing loss but it is important to note that sensorineural hearing loss and mixed hearing loss can also occur as secondary symptoms of the condition. Another symptom present in patients with otosclerosis is tinnitus. The paper carried out a retrospective study of 70 patients diagnosed with otosclerosis in the Department of Otorhinolaryngology of Emergency City Hospital, Timişoara, Romania, between January 2021 to December 2022. Tissue fragments were processed at Service of Pathology by standard Hematoxylin–Eosin staining. The HP diagnosis was completed using Masson’s trichrome staining, Giemsa histochemical staining, and immunohistochemical (IHC) reactions with anti-cluster of differentiation (CD)20, anti-CD3, anti-CD4, anti-CD8, anti-CD34, and anti-CD31 antibodies. The microscopic examination showed a chronic diffuse inflammatory infiltrate that consisted predominantly of mature T-lymphocytes, immunohistochemically positive for CD3, CD4 and CD8. There were also present rare CD20-positive B-lymphocytes. Among the lymphocytes, relatively numerous mast cells were identified, highlighted histochemically by the Giemsa staining. They had numerous purple-violet intracytoplasmic granules. In the connective tissue support, a relatively rich vascular network was identified, consisting of hyperemic capillaries, highlighted immunohistochemically with anti-CD31 and anti-CD34 antibodies. Bone tissues trabeculae showed extensive areas of fibrosis. The collagen fibers were highlighted by Masson’s trichrome staining, being stained in green, blue, or bluish green.

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Section:  Discussionmentioning

confidence: 71%

Otosclerosis under the magnifying glass

Vîrzob¹,

Cloşca²,

Poenaru³

et al. 2023

Rom J Morphol Embryol

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“…Slides were dehydrated again with increasing alcohol concentration, cleared with xylene and mounted with DPX mountant for microscopical analysis of tissue morphology. (40,41).…”

Section: Tissue Morphology Analysis By Haematoxylin and Eosin Stainingmentioning

confidence: 99%

Attenuation of c-Myc expression in breast cancer by hesperidin-mediated stabilization of its promoter proximal G quadruplex region

Choudhury,

Ghosh,

Kumar

et al. 2024

Preprint

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The G-Quadruplex (G4) silencer element (Pu-27), an epigenetic regulator located upstream of the c-Myc promoter, when stabilized and restored to its basal expression, presents a potential avenue for effective anticancer therapy. This study investigates Hesperidin, a citrus flavanone, as a potential breast cancer therapy by targeting the c-Myc G-quadruplex (G4) silencer element, Pu-27. Hesperidin showed strong interaction with Pu-27, indicated by a binding score of -7.241 and the formation of hydrogen bonds with five regions of Pu-27 G4, with a binding free energy of -48.344 kcal/mol. Increasing Hesperidin concentration significantly increased Pu-27 G4 ellipticity and melting temperature, suggesting structural stabilization. Atomic force microscopy revealed higher-order nanostructure formation in Pu-27 G4 with Hesperidin. Functional assays demonstrated reduced Pu-27 primer dimer PCR product and mTFP expression with increasing Hesperidin concentration. Selective cytotoxicity against MDA-MB-231 breast cancer cells, attenuation of clonogenicity and migration, along with downregulation of c-Myc expression both in vitro and in vivo in mice tumor models, further supported Hesperidin's potential as a breast cancer therapeutic. These findings highlight Hesperidin's ability to interact with the c-Myc G4 element, influencing cancer cell behavior, and suggest its promise as a therapeutic agent for breast cancer.

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Subtrochanteric Fracture as First Sign of Metastatic Breast Cancer: Case Presentation

Cited by 2 publications

References 16 publications

Otosclerosis under the magnifying glass

Otosclerosis under the magnifying glass

Attenuation of c-Myc expression in breast cancer by hesperidin-mediated stabilization of its promoter proximal G quadruplex region

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