Subtractive phage display technology identifies zebrafish marcksb that is required for gastrulation

Wang, Yan‐Wu; Wei, Changyong; Dai, Heping; Zhang, Zhu; Sun, Yuhan

doi:10.1016/j.gene.2013.03.028

Cited by 3 publications

(7 citation statements)

References 48 publications

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“…MARCKS serves an important role during embryo development. Blockade of MARCKS protein expression in Xenopus and zebrafish embryogenesis resulted in defective morphogenetic movements of gastrulation by affecting cortical actin formation and cell adhesion, protrusive activity and polarity (9,10). Therefore, MARCKS may serve as a novel biomarker and therapeutic target for cancer, as metastasis is associated with changes in cell morphology and cell migration.…”

Section: Introductionmentioning

confidence: 99%

MARCKSL1 promotes the proliferation, migration and invasion of lung adenocarcinoma cells

et al. 2020

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Lung cancer is the most common cancer in males and females and ~40% of lung cancer cases are adenocarcinomas. Previous studies have demonstrated that myristoylated alanine rich protein kinase C substrate (MARCKS) is upregulated in several types of cancer and is associated with poor prognosis in patients with breast cancer. However, its expression level and role in lung adenocarcinoma remain unknown. Therefore, the aim of the present study was to investigate the expression level and biological functions of MARCKS like 1 (MARCKSL1), a member of the MARCKS family, in lung adenocarcinoma. The expression level of MARCKSL1 was examined in human lung adenocarcinoma tissues and cell lines. MARCKSL1-specific small interfering RNAs effectively suppressed its expression level and significantly inhibited the proliferation, migration and invasion of lung adenocarcinoma cells. Additionally, the role of MARCKSLI in the regulation of metastasis was examined. Silencing MARCKSL1 decreased the expression of the epithelial-mesenchymal transition (EMT)-associated proteins E-cadherin, N-cadherin, vimentin and snail family transcriptional repressor 2, and decreased the phosphorylation level of AKT. The results obtained in the current study suggested that MARCKSL1 promoted the progression of lung adenocarcinoma by regulating EMT. MARCKSLI may have prognostic value and serve as a novel therapeutic target in lung adenocarcinoma.

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Section: Introductionmentioning

confidence: 99%

MARCKSL1 promotes the proliferation, migration and invasion of lung adenocarcinoma cells

et al. 2020

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“…We previously identified zebrafish marcksb which is important for gastrulation movements [25]. To further understand the role of MARCKS family genes in early embryonic development, we examined the expression patterns of all the four members of MARCKS family– marcksa , marcksb , marcksl1a and marcksl1b during early embryogenesis.…”

Section: Resultsmentioning

confidence: 99%

“…The well-studied regulated mucin secretion process via MARCKS involves its PKC and calcium/calmodulin dependent phosphorylation, high binding affinity with F-actin and membrane phosphoinositides, and interaction with intracellular molecular chaperons [20–23]. The MARCKS family proteins have also been reported to play various roles in gastrulation movements in Xenopus [24] and zebrafish [25], and the morphogenesis of neural tube in mouse [26] and chick [27]. However, the potential roles of MARCKS in morphogen secretion and embryonic patterning has never been studied and reported.…”

Section: Introductionmentioning

confidence: 99%

Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b

et al. 2019

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During vertebrate early embryogenesis, the ventral development is directed by the ventral-to-dorsal activity gradient of the bone morphogenetic protein (BMP) signaling. As secreted ligands, the extracellular traffic of BMP has been extensively studied. However, it remains poorly understood that how BMP ligands are secreted from BMP-producing cells. In this work, we show the dominant role of Marcksb controlling the secretory process of Bmp2b via interaction with Hsp70 in vivo. We firstly carefully characterized the role of Marcksb in promoting BMP signaling during dorsoventral axis formation through knockdown approach. We then showed that Marcksb cell autonomously regulates the trafficking of Bmp2b from producing cell to the extracellular space and both the total and the extracellular Bmp2b was decreased in Marcksb-deficient embryos. However, neither the zygotic mutant of marcksb (Zmarcksb) nor the maternal zygotic mutant of marcksb (MZmarcksb) showed any defects of dorsalization. In contrast, the MZmarcksb embryos even showed increased BMP signaling activity as measured by expression of BMP targets, phosphorylated Smad1/5/9 levels and imaging of Bmp2b, suggesting that a phenomenon of “genetic over-compensation” arose. Finally, we revealed that the over-compensation effects of BMP signaling in MZmarcksb was achieved through a sequential up-regulation of MARCKS-family members Marcksa, Marcksl1a and Marcksl1b, and MARCKS-interacting protein Hsp70.3. We concluded that the Marcksb modulates BMP signaling through regulating the secretory pathway of Bmp2b.

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“…The fact that four genes have been maintained, and in addition diverged after genome duplication, suggests they may have acquired different functions during teleost evolution. For the zebrafish marcks genes for which there are in situ hybridization data ( marcksa : Thisse and Thisse, ; marcksb : Wang et al., ; marcksl1a : Thisse et al., ), a widespread distribution from early to late embryonic stages has been shown, with a usually higher expression in the central nervous system. Our quantitative RT‐PCR analysis also showed that all four marcks genes are expressed along the first 3 days of development.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the PCP pathway exerts part of its functions through the modulation of the actin cytoskeleton (Devenport, ). Interestingly, MARCKSB was recently shown to be necessary for zebrafish gastrulation (Wang et al., ), while MARCKS was indicated as an essential intermediate between cortical actin and the PCP pathway during frog gastrulation, where its knockdown using MOs showed severe defects in convergent extension (Iioka et al., ).…”

Section: Discussionmentioning

confidence: 99%

Functional Diversification of the Four MARCKS Family Members in Zebrafish Neural Development

Prieto

Zolessi

2016

J Exp Zool Pt B

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Myristoylated alanin-rich C-kinase substrate (MARCKS) and MARCKS-like 1, each encoded by a different gene, comprise a very small family of actin-modulating proteins with essential roles in mammalian neural development. We show here that four genes (two marcks and two marcksl1) are present in teleosts including zebrafish, while ancient actinopterigians, sarcopterigian fishes, and chondrichtyans only have two. No marcks genes were found in agnaths or invertebrates. All four zebrafish genes are expressed during development, and we show here how their early knockdown causes defects in neural development, with some phenotypical differences. Knockdown of marcksa generated embryos with smaller brain and eyes, while marcksb caused different morphogenetic defects, such as larger hindbrain ventricle and folded retina. marcksl1a and marcksl1b morpholinos also caused smaller eyes and brain, although marcksl1a alone generated larger brain ventricles. At 24 hpf, marcksb caused a wider angle of the hindbrain walls, while marcksl1a showed a "T-shaped" neural tube and alterations in neuroepithelium organization. The double knockdown surprisingly produced new features, which included an increased neuroepithelial disorganization and partial neural tube duplications evident at 48 hpf, suggesting defects in convergent extension. This disorganization was also evident in the retina, although retinal ganglion cells were still able to differentiate. marcksl1b morphants presented a unique retinal phenotype characterized by the occurrence of sporadic ectopic neuronal differentiation. Although only marcksl1a morphant had a clear "ciliary phenotype," all presented significantly shorter cilia. Altogether, our data show that all marcks genes have functions in zebrafish neural development, with some differences that suggest the onset of protein diversification.

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Subtractive phage display technology identifies zebrafish marcksb that is required for gastrulation

Cited by 3 publications

References 48 publications

MARCKSL1 promotes the proliferation, migration and invasion of lung adenocarcinoma cells

MARCKSL1 promotes the proliferation, migration and invasion of lung adenocarcinoma cells

Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b

Functional Diversification of the Four MARCKS Family Members in Zebrafish Neural Development

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