Subtractive hybridisation screen identifies sexually dimorphic gene expression in the embryonic mouse gonad

McClive, Peter J.; Hurley, Tanya M.; Sarraj, Mai; Bergen, Jocelyn A. van den; Sinclair, Andrew H.

doi:10.1002/gene.10231

Cited by 43 publications

(38 citation statements)

References 49 publications

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“…In addition, the extracellular matrix (ECM) plays an essential role in establishing permissive and restrictive scaffolding for endothelial cell migration (33). Sex-specific expression of ECM molecules has been reported in XY vs. XX expression screens (34)(35)(36) and may play a role in domain patterning and directed migration.…”

Section: Individual Endothelial Cells Migrate Via Specific Paths To Tmentioning

confidence: 99%

“…Endothelial cells express neuronal guidance receptors Plexind1 and Unc5b, whereas the somites express their repulsive ligands, Sema3E and Netrins, establishing avascular somite domains (37)(38)(39). Numerous neuronal guidance signaling pathway members, including Robo, Semaphorin, and Netrin, have been identified in embryonic testicular EST libraries (www.ncbi.nlm.nih.gov/UniGene/lbrowse2.cgi?TAXIDϭ10090; dbEST Library Lib.2578, Lib.10027, Lib.2555, and Lib.2594) and general embryonic testicular screens (34)(35)(36). Their role in patterning of the testis vasculature is under investigation.…”

Section: Individual Endothelial Cells Migrate Via Specific Paths To Tmentioning

confidence: 99%

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Four-dimensional analysis of vascularization during primary development of an organ, the gonad

Coveney

Cool

Oliver

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

148

165

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Time-lapse microscopy has advanced our understanding of yolk sac and early embryonic vascularization. However, it has been difficult to assess endothelial interactions during epithelial morphogenesis of internal organs. To address this issue we have developed the first time-lapse system to study vascularization of a mammalian organ in four dimensions. We show that vascularization of XX and XY gonads is a highly dynamic, sexually dimorphic process. The XX gonad recruits vasculature by a typical angiogenic process. In contrast, the XY gonad recruits and patterns vasculature by a novel remodeling mechanism beginning with breakdown of an existing mesonephric vessel. Subsequently, in XY organs individual endothelial cells migrate and reaggregate in the coelomic domain to form the major testicular artery. Migrating endothelial cells respect domain boundaries well before they are morphologically evident, subdividing the gonad into 10 avascular regions where testis cords form. This model of vascular development in an internal organ has a direct impact on the current dogma of vascular integration during organ development and presents important parallels with mechanisms of tumor vascularization.organogenesis ͉ ovary ͉ testis I nsights into vascular development and patterning in internal organs have historically relied on xenograft models and static analysis (1-7). However, this approach does not elucidate dynamic interactions between endothelial cells and other cells of developing organs. Advances in time-lapse imaging have improved understanding of vasculogenesis, flow-induced vascular remodeling, the genetic programming of angiogenesis, and many other facets of vascular development in the mouse and chick yolk sac and during establishment of the body plan in zebrafish (8-17). However, culturing internal organs throughout critical and prolonged periods of development has been difficult. The technical challenges of organ culture, compounded by simultaneous live imaging, is a major hurdle in understanding how endothelial cells remodel and integrate in an internal organ undergoing morphogenesis.For more than a decade the urogenital ridge (UGR) explant model has provided a unique system to analyze morphogenesis of an internal organ (18,19). The fact that the UGR can be successfully explanted to culture at the critical sex-determination stage [11.5 days postcoitum (dpc)], using conditions that maintain the normal morphological structure of the gonad, has been the driving force behind its broad adoption in the sexdetermination field. However, with respect to internal organ vascularization, the UGR has received little attention as a model system. In the UGR explant model the gonad retains contact with the mesonephros, the source of the endothelium, and vascularization ex vivo occurs similarly to vascularization in vivo (20). During the period of vascularization, development of the testis and ovary diverge morphologically. Whereas the XX gonad shows few morphological changes during this period, the XY gonad undergoes dramatic epi...

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Section: Individual Endothelial Cells Migrate Via Specific Paths To Tmentioning

confidence: 99%

Section: Individual Endothelial Cells Migrate Via Specific Paths To Tmentioning

confidence: 99%

Four-dimensional analysis of vascularization during primary development of an organ, the gonad

Coveney

Cool

Oliver

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

148

165

View full text Add to dashboard Cite

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“…Recently, several large scale transcriptome experiments have been performed to study genital ridge gene expression. Several laboratories have employed subtractive screens or microarray analysis to search for sexually dimorphic gene expression between male and female whole embryonic mouse gonads (Bowles et al, 2000a;Grimmond et al, 2000;Wertz and Herrmann, 2000;Menke and Page, 2002;McClive et al, 2003;Smith et al, 2003;Small et al, 2005). More recently, transgenic mouse models expressing fluorescent proteins driven by various lengths of SRY or Sf1 promoters have been developed and used to compare gene expression of specific cell types within the genital ridge around the time of sex determination and differentiation (Boyer et al, 2004;Nef et al, 2005;Beverdam and Koopman, 2006;Bouma et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Presumptive pre‐sertoli cells express genes involved in cell proliferation and cell signalling during a critical window in early testis differentiation

Cory

Boyer

Pilon

et al. 2007

Molecular Reproduction Devel

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In mammals, the pre-Sertoli cell of the male genital ridge is the first cell type to display sex specific differentiation and differential gene expression. The genetic cascade driving the differentiationof pre-Sertoli cells and ultimately testis formationis beginning to be unravelled, but many questions remain. A better understanding of the transcriptome of pre-Sertoli cells immediately after sex determination is essential in order to further understand this differentiationprocess. A mouse model expressing Red Fluorescent Protein (RFP) under the control of a hybrid mouse/pig SRY promoter (HybSRYp-RFP) was used to purify cells from embryonic day 12.0 (e12.0) male genital ridges. To compare the transcriptomes of HybSRYp-RFP cell populations versus age matched whole female genital ridges, RNA was extracted and used to generate molecular probes that were hybridized onto Affymetrix Mouse Genome 430 2.0 microarrays. The expression of genes considered markers for pre-Sertoli cells, including Sox9, Mis, Dhh and Fgf9 were identified within the HybSRYp-RFP expressing cell population, while markers for germ cells (Oct4, SSEA-1) and endothelial cells (Ntrk3) were not identified. In contrast, markers for ovarian somatic cell expression, including Fst and Bmp2, were identified as overexpressed within the ovarian cell population. In a general fashion, genes identified as 2.5-fold over expressed in HybSRYp-RFP expressing cells coded notably for cell signalling and extra cellular proteins. The expression of Sox10, Stc2, Fgf18, Fgf13 and Wnt6 were further characterized via whole mount in situ hybridization (WISH) on male and female genital ridges between e11.5 and e14.5. Sox10, Fgf18, Fgf13 and Stc2 gene expression was detected within the male genital ridges while Wnt6 was found diffusely within both the male and female genital ridges. These data represent the earliest comprehensive microarray expression analysis of purified presumptive pre-Sertoli cells available to date. Mol. Reprod. Dev. 74: 1491-1504, 2007

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“…Busulfan was administered in vivo to deplete developing embryos of germ cells as described (McClive et al, 2003). Briefly, 9.5 dpc pregnant mother mice were injected intraperitoneally with 40 mg/kg of busulfan, in 50% dimethyl sulfoxide (DMSO), and mouse embryos were dissected at 13.5 dpc.…”

Section: Animals and Preparation Of Mouse Embryosmentioning

confidence: 99%

“…A suppression subtraction hybridization screen was conducted on cDNA from mouse testes and ovaries between 12.0 and 12.5 days post coitum (dpc; McClive et al, 2003). Differential clones were sequenced, bioinformatic analysis was performed, and expression patterns were verified by whole-mount in situ hybridization (WISH) on partial 12.5 dpc mouse embryos.…”

Section: Introductionmentioning

confidence: 99%

Novel scavenger receptor gene is differentially expressed in the embryonic and adult mouse testis

Sarraj

McClive

Wilmore

et al. 2005

Developmental Dynamics

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In an effort to understand the mechanisms that underpin gonadal differentiation at the time of sex determination, we identified a cDNA encoding a putative novel testis expressed scavenger receptor, Tesr. Based on its domain structure, we hypothesize that the function of Tesr is similar to that of other scavenger receptors that play roles in phagocytosis of apoptotic cells, cell-cell adhesion, and defense. Tesr mRNA was detected in fetal mouse gonads of both sexes at 11.5 days post coitum (dpc). From 12.0 dpc, Tesr expression rapidly decreased in the female and was maintained in the male. Expression was seen in embryonic mouse sites other than the testis, such as in brain, eye, head, heart, neural arch, and cartilage primordium. Tesr expression in the newborn testis was faint to undetectable, but it increased from 2 days postpartum (dpp) until 15 dpp and was found in a subset of interstitial cells and in germ and Sertoli cells. Tesr mRNA in the adult mouse testis was observed in Sertoli cells, spermatogonia, spermatocytes, round spermatids, and in a subset of interstitial cells. We conclude that Tesr is differentially expressed in the male vs. female embryonic gonad and is expressed in both the ovary and the testes postnatally after 2 dpp. Developmental Dynamics 234:1026 -1033, 2005.

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Subtractive hybridisation screen identifies sexually dimorphic gene expression in the embryonic mouse gonad

Cited by 43 publications

References 49 publications

Four-dimensional analysis of vascularization during primary development of an organ, the gonad

Four-dimensional analysis of vascularization during primary development of an organ, the gonad

Presumptive pre‐sertoli cells express genes involved in cell proliferation and cell signalling during a critical window in early testis differentiation

Novel scavenger receptor gene is differentially expressed in the embryonic and adult mouse testis

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