Subtraction Color Map of Contrast-Enhanced and Unenhanced CT for the Prediction of Pancreatic Necrosis in Early Stage of Acute Pancreatitis

Tsuji, Yoshihisa; Takahashi, Naoki; Fletcher, Joel G.; Hough, David M.; McMenomy, Brendan P.; Lewis, David M.; Vege, Santhi Swaroop; Chari, Suresh T.; McCollough, Cynthia H.; Grant, Katharine L.; Klotz, Ernst

doi:10.2214/ajr.13.10957

Cited by 16 publications

(8 citation statements)

References 41 publications

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“…Most fluorescent screens in zebrafish have utilized either transgenic lines or have a reporter construct or dye microinjected directly into the animals at early stages [8,57–59,69–71]. Gehrig et al microinjected embryos with an array of fluorescent reporter constructs to map the interaction between cis-regulatory elements and core promoters [59].…”

Section: 3d Models Amenable For Imaging-based High Throughput Scrementioning

confidence: 99%

High-throughput fluorescence imaging approaches for drug discovery usingin vitroandin vivothree-dimensional models

Martínez

Titus

Wagner

et al. 2015

Expert Opinion on Drug Discovery

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Introduction High-resolution microscopy using fluorescent probes is a powerful tool to investigate individual cell structure and function, cell subpopulations, and mechanisms underlying cellular responses to drugs. Additionally, responses to drugs more closely resemble those seen in vivo when cells are physically connected in 3D systems (either 3D cell cultures or whole organisms), as opposed to traditional monolayer cultures. Combined, the use of imaging-based 3D models in the early stages of drug development has the potential to generate biologically relevant data that will increase the likelihood of success for drug candidates in human studies. Areas covered The authors discuss current methods for the culturing of cells in 3D as well as approaches for the imaging of whole-animal models and 3D cultures that are amenable to high throughput settings and could be implemented to support drug discovery campaigns. Furthermore, they provide critical considerations when discussing imaging these 3D systems for high throughput chemical screenings. Expert opinion Despite widespread understanding of the limitations imposed by the 2D versus the 3D cellular paradigm, imaging-based drug screening of 3D cellular models is still limited, with only a few screens found in the literature. Image acquisition in high throughput, accurate interpretation of fluorescent signal, and uptake of staining reagents can be challenging, as the samples are in essence large aggregates of cells. The authors recognize these shortcomings that need to be overcome before the field can accelerate the utilization of these technologies in large-scale chemical screens.

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Section: 3d Models Amenable For Imaging-based High Throughput Scrementioning

confidence: 99%

High-throughput fluorescence imaging approaches for drug discovery usingin vitroandin vivothree-dimensional models

Martínez

Titus

Wagner

et al. 2015

Expert Opinion on Drug Discovery

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“…The sensitivity and specificity of perfusion CT for predicting necrotizing pancreatitis was given with 87.5% and 100%, respectively. 15,16 These data suggest that perfusion CT might be an alternative measure to the clinical scores and CECT for risk stratification in SAP.…”

Section: Current Management Issuesmentioning

confidence: 94%

Acute Pancreatitis—Progress and Challenges

Afghani¹,

Pandol²,

Shimosegawa³

et al. 2015

Pancreas

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An international symposium entitled “Acute pancreatitis: progress and challenges” was held on November 5, 2014 at the Hapuna Beach Hotel, Big Island, Hawaii, as part of the 45th Anniversary Meeting of the American Pancreatic Association and the Japanese Pancreas Society. The course was organized and directed by Drs. Stephen Pandol, Tooru Shimosegawa, Robert Sutton, Bechien Wu, and Santhi Swaroop Vege. The symposium objectives were to: (1) highlight current issues in management of acute pancreatitis, (2) discuss promising treatments, (3) consider development of quality indicators and improved measures of disease activity, and (4) present a framework for international collaboration for development of new therapies. This article represents a compilation and adaptation of brief summaries prepared by speakers at the symposium with the purpose of broadly disseminating information and initiatives.

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“…Over time, the intrapancreatic fluid becomes reabsorbed, and nonviable necrotic tissues liquefy [18,19]. Frank necrosis may take 24-48 hours to develop [7,20,21], and the sensitivity of CECT for detection of pancreatic necrosis within 72 hours of disease onset is approximately 60-70% [22,23]. Ideally, imaging should be performed at least 72 hours after symptom onset [6].…”

Section: Limitations Of Early Necrosis Detectionmentioning

confidence: 99%

Acute Pancreatitis: Revised Atlanta Classification and the Role of Cross-Sectional Imaging

Zhao

Adam

Keswani

et al. 2015

American Journal of Roentgenology

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Subtraction Color Map of Contrast-Enhanced and Unenhanced CT for the Prediction of Pancreatic Necrosis in Early Stage of Acute Pancreatitis

Abstract: A subtraction color map is accurate in the diagnosis of pancreatic necrosis in the early stage of acute pancreatitis.

Cited by 16 publications

References 41 publications

High-throughput fluorescence imaging approaches for drug discovery usingin vitroandin vivothree-dimensional models

High-throughput fluorescence imaging approaches for drug discovery usingin vitroandin vivothree-dimensional models

Acute Pancreatitis—Progress and Challenges

Acute Pancreatitis: Revised Atlanta Classification and the Role of Cross-Sectional Imaging

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